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This is a place on the DNA molecule that "advertises" for the attachment of the Polymerase for transcription of a gene
3. Gene expression is controlled differently in specific cell types
Which of the following statements is true? 1. All genes are always expressen in all cell types. 2. Different cell types have different sets of genes. 3. Gene expression is controlled differently in specific cell types. 4. Gene expression is random and occurs by chance.
A release factor enters the A site of the ribosome and the whole complex comes apart
During the assembly of a protein, when a stop codon enters the ribosome, this happens:
These are the enzymes used to remove non-coding portions of the mRNA before exit from the nucleus
A section of DNA that has many _____ repeats is weaker than one that contains many _____ because of the number of hydrogen bonds
The promoter region has this segment that the transcribing enzymes recognized as a binding spot
A gene coding for turning the colonies green, a gene coding for resistance to ampicillin
What 2 genes are carried by the pGLO plasmid?
What nutrient substance acts as a chemical switch to activate one of the genes on our plasmid?
E. Coli does not have many cells, making it easier to transform.
Why is E. Coli a good choice for an organism to transform?
E. Coli will not fluoresce and the pGLO will neutralize the effects of ampicillin
What do you expect to see on the +pGLO LB/Amp plate?
The plate with arabinose will turn glowing green.
What do you expect to see on the +pGLO LB/Amp/Ara plate?
some movement of water in both directions, but no net movement
If a dialysis bag with distilled water inside of it were placed in distilled water, what would happen?
This is the form of hereditary material that is present in the nucleus most of the time, it is loose and looks grainy through a microscope
The increased concentration of a particular ion on one side of a cell membrane in order to do work is called
In the diffusion lab experiment, with the dialysis bags, we used _____ to measure the rate of diffusion
synthesis of lipids, metabolism of carbs, and detoxification of drugs and poisons
List the functions of the smooth endoplasmic reticulum
size, charge, polarity
List three characteristics of molecules that determine permeability for that molecule
fungi, archaebacteria, plants
List three organisms that have cell walls (there are more than the ones I have listed here)
These were once thought to have been a free living prokaryote that could metabolize oxygen
Meiosis I is sometimes referred to as the ______ stage of meiosis because the chromosome number goes from 2n to 1n
In which phase do the chromosomes line up 2x2 or side-by-side with their partner down the middle
Partners on a dance floor coming together and embracind is how we describe this process for homologous pairs
When bits of chromatid segments break off and switch places with the partner, this is called _______
chromosomes line up in the center of the cell
Each of the following occurs by the end of prophase except: (coiling of the chromosome becomes tighter, nucleus disappears, chromosomes line up in the center of the cell, nuclear envelope breaks down)
In mitosis, the string-like structure that is important to moving the chromatids apart is the _____
interphase, mitosis, cytokinesis
For a cell to go through a complete reproductive cycle the stages occur in the following order and have the approximate percentage of time spent in each phase or stage: 80% _____, 18% _____, 2% _____
In an animal cell the cell membrane begins to pinch in forming a _____ in preparation for cytokinesis
After DNA replication, the exact copies remain attached to each other, now being called _____
Triglycerides release _____ times the energy per gram as carbohydrates do when combined with _____
steroid (Manny Ramirez)
This type of lipid does not have a carbon chain for its backbone; instead it has 4 carbon rings
The phosphate group on the DNA and RNA allows DNA and RNA to be manipulated because it gives the molecule this property: a _____
One half of a DNA molecule arranged in an upside down orientation compared to the other half is called _____
The chemical reaction that involves two amino acid structures side-by-side and create a condensation reaction forms what type of bond?
These type of proteins are chemical messengers that are made in the glands and affect only target tissues
When solute levels are higher outside of a cell compared to the cytoplasm, the outside is considered _____
expand because the cytoplasm is hypertonic
If a blood cell is placed in distilled water, the cell will _____
the random movement of molecules from a high concentration to a low concentration is called _____
human skin cells
All have cell walls except: (human skin cells, E. coli, portabella mushrooms, extremophiles)
Water is considered a _____ because it's pH fluctuates drastically in the presence of acid or base
The pH scale is an exponential logarithmic scale in that when you move from any number to the next on the scale, the acidity or alkanity increases or decreases by a power
The electrons of an atom occupy more space than the protons and neutrons because of their _____
has a slightly positive charge near the Hydrogens and a slightly negative charge near the oxygen
Water is polar. This means that it
When salt dissociates in water, and each atom now carries a charge, this process is called _____
Two strands of DNA that each carry the genes for the same traits in the same location along their lengths are called what?
The second site to be filled by a tRNA carrying an amino acid and is the site that continuously gets filled as it becomes vacant
This procedure causes a chemical change in the cell membrane making it more likely to take up plasmid
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