*H-bonding between 1st and 2nd position of the codon and the 3rd and 2nd position of the anticodon occur via Watson-Crick base pairing
*Base pairing between the 3rd position of the codon and the 1st position of the anticodon is less constrained (wobble position)
*For example, G, U, and I (inosine) in the wobble position of the anticodon can base pair with C/U, A/G, and C/A/U in the codon, respectively.
*Wobble base pairing reduces the number of tRNA genes that an organism must make to carry out translation.
*It helps protect against mutations that might inactivate tRNA genes