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fragment of DNA composed of sequences originating from at least two different sources
enzymes that are able to cleave double-stranded DNA into fragments at specific sequences; also known as restriction enzymes
a specific sequence within double-stranded DNA, usually palindromic and consisting of four to eight nucleotides, that a restriction endonuclease recognizes and cleaves
fragment end of a DNA molecule with short single-stranded overhangs, resulting from cleavage by a restriction enzyme
fragment ends of DNA molecule that are fully base paired, resulting from cleavage by a restriction enzyme
enzymes that add a methyl group to one of the nucleotides found in a restriction endonuclease recognition site, altering its chemical composition
separation of charged molecules on the basis of size by sorting through a gel meshwork
region in plasmid that has been engineered to contain recognition sites of a number of restriction endonucleases
a fragment of DNA that has been introduced into a foreign cell resulting in exact copies of the original DNA fragment being made when the foreign cell replicates and divides
a cell that has taken up a foreign plasmid or virus and whose cellular machinery is being used to express the foreign DNA
polymerase chain reaction
amplification of DNA sequence by repeated cycles if strand separation and replication
short sequences of DNA nucleotides that are complementary to the opposing 3' to 5' ends of the DNA target sequence that is to be replicated
DNA polymerase, extracted from thermus aquaticus, that is able to withstand high temperatures
any difference in DNA sequence, coding or non-coding, that can be detected between individuals
a technique in which DNA regions are digested using restriction endonucleases and subjected to radioactive complementary DNA probes to compare the differences in DNA fragment lengths between individuals
sanger dideoxy method
DNA sequencing technique based on DNA replication that uses dideoxy nucleoside triphosphates
nucleoside triphosphate whoe ribose sugar does not possess a hydroxyl group on the 2' and the 3' carbon
the alteration of genetic sequence in an organism to prevent or treat a genetic disorder
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