Genetics 2: slides 14-27 (end)

Which of the following relaxes supercoiling?
1. Helicase
2. DNA topoisomerase
3. ssb
4. primase
5. dna polymerase III
6. dna polymerase I
7. dna ligase
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Terms in this set (34)
which direction does the new daughter strand that is built from the template strand elongate in?5'-3'in a faulty DNA strand, RNA is found to be interspersed within. What step of DNA replication went wrong?the RNA primers were not removed by DNA polymerase Idefine replisomegroup of proteins that are a part of a complex needed for DNA synthesishow many replisomes are in dna synthesis and where are they found?two: one at each replication forkwhat is the difference in the job between both replisomes?one replisome carries out dna synthesis following the leading strand and the other does it following the lagging strandthe replisome on the lagging strand carries out dna synthesis continuously in the 5'-3' direction. true or falsefalse: it carries out synthesis discontinuously in the 5'-3' direction because it makes the okazaki fragementsthe replisome on the leading strand carries out dna synthesis continuously in the 5'-3' direction. true or falsetruewhat joins together okazaki fragments and what removes the primers?ligase joins them and DNA polymerase I removes primerson the leading strand, which direction does dna polymerase iii read? what direction does the new dna strand elongate?read: 3'-5' elongates: 5'-3'on the lagging strand, which direction does dna polymerase iii read? what direction does the new dna strand elongate?reads: 5'-3' elongates: 3'-5'when DNA polymerase I removes the primers which are made of RNA, what does it replace them with?dna nucleotidesdescribe how dna polymerase I does it's job using exonuclease and polymerase. The job is not done once dna polymerase I replaces the rna nucleotides in the primer with dna nucleotides. How does DNA ligase finish the job?-dna polymerase attaches to the single-stranded gap betweek okazaki fragments and uses a 5'->3' exonuclease to remove the rna nucleotides and uses a 5'->3' polymerase to add dna nucleotides - When dna polymerase is done, the single-stranded gap (now dna-dna) is still there. ligase comes and creates the final phosphodiester bond between the two nucleotides that joins the okazaki fragmentswhat does dna polymerase I do when there is a nucleotide match error?it stops and reverses replication to remove the incorrect nucleotide and replaces it with the correct ondescribe how topoisomerase does its jobreplication fork causes supercoiling in the double helix (region that is not replicated yet and is still double-stranded). topoisomerase goes to a supercoil site and cleaves the dna strand which releases the pressure and allows the strand to rotate to remove the supercoil. Topoisomerase then rejoins the dna strandswhat type of cells in the body have telomerasesgerm-line cellswhat do telomerases doenzyme that adds repetitve dna to the ends of telomerestrue or false: all living cells have telomeres that solves the provlem of chromosome shortening after each replicationfalse. only eukaryotic cells have telomeres.why dont prokaryotic cells need telomerestheir dna is circular therefore there is no issue of primers found at the end of the lagging strand.why does linear dna need telomeresbecause primers that are found at the end of the lagging strand leave a gap once they are removed and not replaced with dna nucleotides which over time would shorten the dnahow do telomeres solve the problem of dna shorteninginstead of coding dna shortening, the end of the telomere shortens however the dna in telomeres is non-coding, so no damage occurs to the cell or bodywhat is the purpose of the t-loop in telomeresprotects them from enzymatic degradationwhat happens once a telomere is fully gone from repeated replications?cell undergoes apoptosis to avoid catastrophic dna failure.describe the Hayflick limitnumber of replication cycles in a cell's life span which is 50-70 cyclesDNA pol 3 adds nucleotides to which end of DNA3'