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Question

A chromatographic procedure separates 4.0 mg of unknown mixture on a column with a length of 40 cm and a diameter of 0.85 cm.

(a) What size column would you use to separate 100 mg of the same mixture?

(b) If the flow is 0.22 mL/min on the small column, what volume flow rate should be used on the large column?

(c) The mobile phase occupies 35% of the column volume. Calculate the linear velocity for the small column and the large column

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In this task we have a chromatographic procedure which separates 4 mg of unknown mixture on a column with a length of 40 cm and a diameter of 0.85 cm.

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