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a. The original constitutive operator mutations in the lac operon were all base changes in O1.O_{1} . Why do you think mutations in O2O_{2} or O3O_{3} were not isolated in these screens? b. Explain how a mutagen that causes small insertions could produce an OcO^{c} mutation. c. Would the OcO^{c} mutation described above in part b be sensitive to lacls?\mathrm{lacl}^{s} ? Why or why not?

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a) Because the individual mutations in either O2O_2 or O3O_3 have very small effects on synthesis levels. That comes fact that the main region which binds the repressor is O1O_1 and the other 2 parts only help in partial loop formation afterwards.

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