Question

Explain how the gene for chymosin was isolated and how the technique could be applied to isolating other genes:

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Chymosin is extracted from the stomach of of young mammals that fed on milk. The RNA codons are determined and transcribe mRNA to DNA using reverse transcriptase. The DNA sequence is amplified using PCR. This is then used to combine with the plasmid of E.coli\textit{E.coli}. The transformed bacteria is used to make cheese. This breakthrough helps in making treatments for diseases that have difficulty in producing. Like the treatment for diabetes mellitus. Insulin from human is combined with E.coli\textit{E.coli} to mass produce insulin injections needed by diabetic patients.

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