If the bacteria E. coli synthesize DNA at a rate of 100,000 nucleotides per min and it takes 30 min to replicate the DNA, how many base pairs are in an E. coli chromosome?
Step 11 of 2
Given that synthesize DNA at a rate of 100,000 nucleotides per min and it takes 30 min to replicate the DNA, we simply multiply them and divide it by 2 to get the number of base pairs.
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Bacterial genomes such as that of E. coli typically have only a single origin of replication, from which replication proceeds bidirectionally. PolIII, the DNA polymerase responsible for replicating the E. coli chromosome, synthesizes DNA at a rate of about 1000 nucleotides per second. a. From this information, estimate the minimum generation time of E. coli. b. Under optimal conditions, E. coli have been observed to divide in as little as 17 minutes. Speculate how this might be possible, given your answer to part (a).
An outbreak of E. coli infections in August of 2010 occurred in three Washington state day care centers. There were eight confirmed and six suspected cases of E. coli, with over 70 children awaiting test results. Outbreaks of E. coli infections for 2009 are reported to be less than 1 per 100,000, down from 2.5 per 100,000 reported earlier. Using the rate of 1 per 100,000, find the following probabilities. a. What is the probability that at most two outbreaks per 100,000 are reported across the United States this year? b. What is the probability that more than three outbreaks per 100,000 are reported across the United States this year?
Now that the sequence of the entire E. coli K12 strain genome (roughly 5 Mb) is known, you can determine exactly where a cloned fragment of DNA came from in the genome by sequencing a few bases and matching that data with genomic information. a. About how many nucleotides of sequence information would you need to determine exactly where a fragment is from? b. If you had purified a protein from E. coli cells, roughly how many amino acids of that protein would you need to know to establish which gene encoded the protein? c. You determine 100 nucleotides of sequence of genomic DNA from a different E. coli strain, but you cannot find a match in the E. coli K12 genome sequence. How is this possible?
The generation time for bacteria is the time that it takes for the population to double. The generation time G for a specific type of bacteria can be found using experimental data and the formula
where t is the time period, b is the number of bacteria at the beginning of the experiment, and f is the number of bacteria at the end of the experiment. E. coli are fast growing bacteria. If 6 E. coli can grow to 1296 in 4.4 hours, what is the generation time of E. coli?