Question

List the mechanistic steps of DNA synthesis starting with the primed template and deoxynucleoside triphosphate.

Solution

Verified

i. Synthesis of an RNA primer ii. Annealing of complementary nucleotides by hydrogen bonding iii. Formation of phosphodiester bond iv. Release and hydrolysis of pyrophosphate by pyrophosphatase. v. Nucleotide is now paired with the template.

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