Question

One of the steps in the biological pathway for carbohydrate metabolism is the conversion of fructose 1,6-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Propose a mechanism for the transformation.

Solution

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Aldolase is a glycolytic enzyme that catalyzes the conversion of fructose 1-6-diphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone 3-phosphate via the glycolysis metabolic pathway.

Fructose 1-6-diphosphate in basic conditions is broken to two compounds, one is glyceraldehyde 3-phosphate. The other must be protonated to keto form, dihydroxyacetone 3-phosphate.

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