Question

If a DNA sample contains 25 percent adenine (A), what percent of thymine, cytosine, and guanine must it contain? Use Chargaff's rule to construct your answer.

Solution

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Erwin Chargaff first noticed that the percentage of adenine and thymine, as well as guanine and cytosine, is almost the same in every DNA molecule. This is known as the Chargaff's rule. If A = 25%, we can conclude that T = 25%. The remaining 50% (100-25-25) of the DNA is composed out of guanine and cytosine (G+C=50%). Since G = C, therefore G=25% as well as C=25%.

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