What is the purpose of a genomic library?

You are attempting to insert a gene for saltwater tolerance into a plant by using the $Ti$ plasmid. In addition to the desired gene, you add a gene for tetracycline resistance ($tet^R$) to the plasmid. What is the purpose of the $tet^R$ gene?

Describe five ways of getting DNA into a cell.

Describe five ways of getting DNA into a cell.

For what is each of the following used in PCR: primer, DNA polymerase, 94$\degree$C?

Match the following choices to the statements in questions a. antisense
b. clone
c. library
d. Southern blot
e. vector
Pieces of human DNA stored in yeast cells.

List at least two examples of the use of rDNA in medicine and in agriculture.

Identify the causative agent, reservoir, symptoms, and treatment for cryptococcosis.

Poliomyelitis is an acute infection of which system?

Provide the following information on poliomyelitis: etiology, method of transmission, symptoms, prevention. Why aren’t the Salk and Sabin vaccines considered treatments for poliomyelitis?

Outline the steps in PCR, and provide an example of its use.

Why is a vector used in recombinant DNA technology?

Describe the use of plasmid and viral vectors.

You have a small gene that you want replicated by PCR. You add radioactively labeled nucleotides to the PCR thermal cycler. After three replication cycles, what percentage of the DNA single strands are radioactively labeled?
a. $0$%
b. $12.5$%
c. $50$%
d. $87.5$%
e. $100$%