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Past Papers Revision
Terms in this set (37)
The sanger method for DNA sequencing utilizes:
Translation of the following mRNA sequence will yield a protein with ______ amino acid residues.
The citric acid cycle and the usea cycle are linked via:
The aspartate-arginosuccinate shunt
What is the gross energetic budget of the two phases of glycolysis?
Preparatory phase: 2ATP; Pay-off phase: 4 ATP and 2NADH
Why is the glucose molecule phosphorylated during glycolysis:
To keep it within the cell and to lower the activation energy of glycolytic reactions
What is the role of coenzyme Q in the electron transfer chain?
It transfers electrons from complex I to complex III
How many acetyl-coA moelcules are produced following the oxidation of a fatty acid that is 48 carbons long?
Free fatty acids in the bloodstream are:
Carried by the protein serum albumin
Individuals with untreated diabetes can suffer from:
High blood sugar levels, weight gain and ketosis
Amino acds are comprised of:
An amino group, a carboxyl group and a functional group
Five DNA fragments (A: 1000 bp; B: 2000 bp; C: 2500 bp; D: 3500 bp; E: 5000 bp) were subjected to agarose electrophoresis. At the end of the electrophoresis, which DNA fragment will be closest to the negative electrode?
The complementary RNA sequence of 5'- ATAGCCCTGCAATTCGATT- 3' is:
Fill in the blanks
Insulin is secreted in the ________ as a response to ________ sugar levels.
When triglycerides are broken up, the long hydrocarbon chain enters...
Tom wants to make a 1.2 mg/ml dilution of bovine serum albumin (BSA) at a total volume of 1 ml. He has been given a stock BSA solution at a concentration of 6 mg/ml. How much stock BSA (in microliters) must he use?
Calculate the protein concentration of a sample with absorbance 6 using the equation: y = 2x - 0.5. (Absorption was plotted on the x-axis)
In the DNA double helix the stacking nucleotide bases interact through:
Van der Waal interactions
Assuming that the average amino acid residue contributes 110 Daltons to overall the peptide molecular weight, what will be the weight (in Daltons) of a protein coded by a mRNA that is 990 nucleotides long?
In the electron transport chain, NADH donates two electrons to:
Exist as zwitterions at pH 7.4
Form peptide bonds in proteins
Differ in their functional groups
Are full deprotonated at pH 14
Helen wants to make a 0.75 mg/ml dilution of bovine serum albumin (BSA) at a total volume of 2 ml. She has been given a stock BSA solution at a concentration of 4 mg/ml. How much stock BSA (in microliters) must she use?
Five DNA fragments (A: 4500 bp; B: 4700 bp; C 3500 bp; D: 3548 bp; E: 5000 bp) were subjected to agarose electrophoresis. At the end of the electrophoresis, which DNA fragment will be closest to the negative electrode?
The complementary DNA sequence of 5'- ATAGCCCTGCAATTCGATT- 3' is:
The enzymes trypsin and chymotrypsin:
Degrade proteins in the small intestine
Fill in the blanks:
_________ increases cholesterol biosynthesis whereas _______ decreases cholesterol biosynthesis.
The carnitine shuttle is involved in the:
Transfer of large fatty acids across the mitochondrial membranes
Fats are used for long-term energy storage because:
Carbons in fatty acids are more reduced and thus carry more energy
The energy to phosphorylate ADP is provided by:
The flow of protons down the electrochemical gradient
In the Cori cycle:
Lactase is transported from the active muscle to the liver to be converted to glucose
In comparison to DNA, RNA is more susceptible to hydrolysis due to:
The presence of a hydroxyl group at carbon 2 of the D-ribose sugar
Which of the following codons, codes for methionine
Is an anabolic pathway which shares 7 out of the 10 enzymatic reactions with glycolysis
Although composed of a single strand of RNA, each molecule contains several short, double-helical regions
Any given tRNA will accept only one specific amino acid
There is at least one tRNA for each of the 20 amino acids
If a person's urine contains unusually high concentrations of urea, which one of the following diets has he or she probably been eating recently?
Very low carbohydrate, very high protein
Beta oxidation is important because it yields:
A certain bacterial mRNA is known to represent only one gene and to contain about 900 nucleotides. If you assume that the average amino acid residue contributes 110 Da to the peptide molecular weight, the largest polypeptide that this mRNA could code for would have a molecular weight of approximately:
A plasmid used in DNA cloning MUST have:
Antibiotic resistance region
THIS SET IS OFTEN IN FOLDERS WITH...
Lecture 5 DNA, RNA and genetic code
Lecture 6 & 7.3 Translation
Lecture 1 Introduction to Biochemistry
Lecture 8 Proteins
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