This set will teach you about the basic instrumentation covered on the MLT/MLS ASCP registry exam. We will go over multiple different analyzers and the methodologies for each test.
Terms in this set (30)
Explain the principle of a normal sandwich ELISA
The primary (or capture) antibody is bound to a solid phase. Then the sample is introduced and the antigen binds to the primary antibody. Then the cuvette is washed out and only the bound antibody and antigen are left. Then a labeled secondary (or test) antibody is added which binds to the antigen then the label is detected.
an excess of antibodies which results in a lack of agglutination/precipitation despite the presence of an antigen. The abundance of antibodies causes each antigen to bind a different antibody, which impairs the ability for the antibodies to crosslink and cause agglutination/precipitation. One antibody must bind two different antigens to crosslink.
an excess of antigen which results in a lack of agglutination/precipitation despite the presence of an antigen. The abundance of antigen floods the binding sites of the antibodies and impairs the ability to crosslink and cause agglutination/precipitation.
How do interfering antibodies cause false negatives?
They bind to the antigen instead of the test antibody
How to interfering antibodies cause false positives?
They bind to something that you're not interested in which is attached to the solid phase. Then if the testing antibody is a labeled anti-IgG, then the interfering antibody will be detected and produce a false signal
OTHER SETS BY THIS CREATOR
Pediatric Milestones and Immunization schedule
Quick Obstetrics Facts
Principles of Carbohydrate Testing, Lipid Testing, and Protein Analysis
Principles of the laboratory, lab math, and instrumentation
THIS SET IS OFTEN IN FOLDERS WITH...
1. Laboratory Basics and Pertinent Safety
3 - Carbohydrates