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Study sets matching "221 micr microbiology"

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Study sets matching "221 micr microbiology"

100 terms
MICR 221: Medical Microbiology
Commensalism
Mutualism
Pathogenesis
Parasitism
A symbiotic relationship in which one organism benefits and t…
A symbiotic relationship in which both organisms benefit
A symbiotic relationship in which one organism is harmed (the…
A symbiotic relationship in which one organism is harmed (the…
Commensalism
A symbiotic relationship in which one organism benefits and t…
Mutualism
A symbiotic relationship in which both organisms benefit
MICR 221: Medical Microbiology
Commensalism
Mutualism
Pathogenesis
Parasitism
A symbiotic relationship in which one organism benefits and t…
A symbiotic relationship in which both organisms benefit
A symbiotic relationship in which one organism is harmed (the…
A symbiotic relationship in which one organism is harmed (the…
Commensalism
A symbiotic relationship in which one organism benefits and t…
Mutualism
A symbiotic relationship in which both organisms benefit
38 terms
MICR 221: Environmental Microbiology
How do we define a species?
Challenges to species definition
What is the importance of horizontal g…
What are other challenges to definitio…
- metazoans and some unicellular eukaryotes are often defined…
- horizontal gene transfer - DNA can be transferred by transf…
- Ford Doolittle... - common ancestral community of primitive ce…
- divergence through mutation and natural selection: random m…
How do we define a species?
- metazoans and some unicellular eukaryotes are often defined…
Challenges to species definition
- horizontal gene transfer - DNA can be transferred by transf…
61 terms
MICR 221: Majury - Clinical Microbiology
What is the difference between acute c…
How much water do we use per day?
What is the biggest form of water usage?
What is the global water quality?
Acute Care:... - patient care... - disease diagnosis, treatment... - u…
- 1.4 billion L in toronto... - 0.03% of lake ontario... - 329L per…
- only >1% is for drinking... In USA:... - 46% is industrial... - 41%…
- reservoir for parasitic infections, vector-borne diseases... -…
What is the difference between acute c…
Acute Care:... - patient care... - disease diagnosis, treatment... - u…
How much water do we use per day?
- 1.4 billion L in toronto... - 0.03% of lake ontario... - 329L per…
MICR221
Phototroph
Chemotroph
Autotroph
Heterotroph
Derive energy from the sun
Energy derived from oxidation of chemical compounds (organic…
Utilise only inorganic carbon in the form of CO2
Utilise organic carbon ( proteins, carbohydrates and lipids)
Phototroph
Derive energy from the sun
Chemotroph
Energy derived from oxidation of chemical compounds (organic…
157 terms
MICR221
PTS transport
PTS transport 2 types of components
phosphorylating ensures
PEP becomes
multicomponent and phosphorylates substrate
non-specific and specific
the substrate cannot efflux
pyruvate
PTS transport
multicomponent and phosphorylates substrate
PTS transport 2 types of components
non-specific and specific
273 terms
MICR221
metabolic diversity
localised degradation
extremozymes
a amylase
been around for a long time to evolve, mean can grow on anyth…
must attach at a localised point where enzymatic activity occ…
in thermophilic bacteria - can function under extremely high…
breaks a14 bonds in starch
metabolic diversity
been around for a long time to evolve, mean can grow on anyth…
localised degradation
must attach at a localised point where enzymatic activity occ…
53 terms
MICR221: Module 3 - Medical Microbiology (Lecture 11-17)
Symbiosis
Commensalism
Mutualism
Pathogenesis
The living together of two organisms e.g. commensalism, mutua…
Symbiotic relationship where one organism benefits, and the o…
Symbiotic relationship in which both organisms benefit e.g. i…
Symbiotic relationship in which one organism is harmed (the p…
Symbiosis
The living together of two organisms e.g. commensalism, mutua…
Commensalism
Symbiotic relationship where one organism benefits, and the o…
272 terms
MICR221
metabolic diversity
localised degradation
extremozymes
a amylase
been around for a long time to evolve, mean can grow on anyth…
must attach at a localised point where enzymatic activity occ…
in thermophilic bacteria - can function under extremely high…
breaks α1,4 bonds in starch
metabolic diversity
been around for a long time to evolve, mean can grow on anyth…
localised degradation
must attach at a localised point where enzymatic activity occ…
85 terms
MICR221
Membrane Proteins
Active transport
Classes of bacterial active transporters
Major Facilitator Superfamily
The mechanisms for transporting molecules through the lipid b…
The movement of materials through a cell membrane against the…
1. Major Facilitator Superfamily (MFS)... 2. ATP- Binding Casset…
A group of transporters that use proton motive force. 12 -14…
Membrane Proteins
The mechanisms for transporting molecules through the lipid b…
Active transport
The movement of materials through a cell membrane against the…
27 terms
MICR221: Lab 5
Staphylococcus
Staphylococccus epidermis
S.aureus
T1
faculatative anaerobic, non-motile cocci 0.5-1.5 micrometers…
endocartiditis and infection in patients with lowered resista…
causes boils, abscesses and wound infections , pneumonia, tox…
Fuzzy, edge, large
Staphylococcus
faculatative anaerobic, non-motile cocci 0.5-1.5 micrometers…
Staphylococccus epidermis
endocartiditis and infection in patients with lowered resista…
118 terms
MICR221: Viriology
Viruses
Virion
First virus to be discovered?
What is the structure of a virion?
Cellular microorganisms, which cannot survive without a host,…
Complete virus particle
Tabacco mosaic Virus (TMV)
-Constructed of a nucleocapsid
Viruses
Cellular microorganisms, which cannot survive without a host,…
Virion
Complete virus particle
85 terms
MICR221: Immunology
Innate Immunity
Adapative immunity
Example of antigen uptake
Dendritic cells
-Physical and chemical barriers... -Rapid response, but low-spec…
-Slower initial response, but memory response rapid and highl…
Dendritic cells, Macrophage and Neutrophils
This is the most potent of all antigen-presenting cells (APC)…
Innate Immunity
-Physical and chemical barriers... -Rapid response, but low-spec…
Adapative immunity
-Slower initial response, but memory response rapid and highl…
84 terms
MICR221: Bacterial Powerhouse
What Polymers do bacteria grow on?
Protease
Cellulases
Lipases
Polysaccharide, Protein, Organic material, Cellulose and Starch
Peptide
Breaks down cellulose (B-1-4 linkage)
Fats and Glycerol
What Polymers do bacteria grow on?
Polysaccharide, Protein, Organic material, Cellulose and Starch
Protease
Peptide
10 terms
MICR 221: Lab
Turbidity
How do you dislodge any growth that ha…
What are the two ways structure and mo…
Smearing bacteria growing in a liquid…
cloudiness. Indicative of the presence of bacterial growth
Shake the universal gently
1) living unstained organisms usually in liquid preparations…
using a sterile loop to place one or two drops of the liquid…
Turbidity
cloudiness. Indicative of the presence of bacterial growth
How do you dislodge any growth that ha…
Shake the universal gently
47 terms
MICR221 Module 1
Old View
Extremozymes
Starch
Amylase
free enzymes produced in large quantities
enzymes that work at high temperature
white, tasteless and odorless powder insoluble in water or al…
breaks alpha 1,4 link
Old View
free enzymes produced in large quantities
Extremozymes
enzymes that work at high temperature
178 terms
MICR221 Module 3
What benefits do commensals of mutuali…
Commensalism?
Pathogenesis?
Parasitism?
- Nutrition, Protection against pathogens, development in the…
A symbiotic relationship in which both organisms benefit
A symbiotic relationship in which one organism is harmed (pat…
A symbiotic relationship in which one organism is harmed (par…
What benefits do commensals of mutuali…
- Nutrition, Protection against pathogens, development in the…
Commensalism?
A symbiotic relationship in which both organisms benefit
34 terms
MICR221: LAB 1
Aseptic Technique
Flame sterilisation
Flame sterilisation in a wire loop
Flame sterilising the open rim of a bo…
is set of specific practices and procedures performed under c…
is a routine procedure carried out in many microbiological, c…
Loop in a Bunsen burner flame until it grows red ensures that…
heat creates a convection currents out of the bottle as well…
Aseptic Technique
is set of specific practices and procedures performed under c…
Flame sterilisation
is a routine procedure carried out in many microbiological, c…
25 terms
MICR221: Lab 3
List some of the sources of error when…
Viable count
Why does the sample need to be diluted?
What is the major advantage of viable…
-Medium employed cannot support growth of all viable microorg…
relies on the growth of individual cells present in the initi…
so that the cells plated falls within a countable range (25-2…
Only account for living cells
List some of the sources of error when…
-Medium employed cannot support growth of all viable microorg…
Viable count
relies on the growth of individual cells present in the initi…
9 terms
micr 221 Lab 4
Mycology?
Fungi under a microscope?
How is nutrient extracted from the med…
What produces asexual spores?
Study of Fungi-eukaryotic multicellular organisms
Mass of intertwining branching threads called mycelia (singul…
Vegetative mycelia grow on the surface or within the growth m…
From the sections of the vegetative mycelia are specialised f…
Mycology?
Study of Fungi-eukaryotic multicellular organisms
Fungi under a microscope?
Mass of intertwining branching threads called mycelia (singul…
93 terms
MICR221 Module 2
Mean generation time
What are the physical factors of bacte…
How do anaerobes make ATP?
How can oxygen be toxic?
The time it takes for one cell (and therefore the whole popul…
Gaseous environment, temperature, pH and osmotic pressure
Anaerobic respiration: uses ETC, but final electron acceptor…
O2 can be converted by metabolic enzymes into highly reactive…
Mean generation time
The time it takes for one cell (and therefore the whole popul…
What are the physical factors of bacte…
Gaseous environment, temperature, pH and osmotic pressure
30 terms
MICR221 Module 1
What type of complex polymers do bacte…
What is the new view by which bacteria…
What is the old view by which bacteria…
What are some enzymes used by bacteria…
Plant and animal tissue, excretory end products, antibiotics,…
Local degradation - specific genes turned on to produce enzym…
Free enzymes produced in large quantities.
proteases, cellulases, lipases, xylanases, amylases, pectinases
What type of complex polymers do bacte…
Plant and animal tissue, excretory end products, antibiotics,…
What is the new view by which bacteria…
Local degradation - specific genes turned on to produce enzym…
45 terms
MICR221: Lab 2
Classification
Nomenclature
Identification
Culture media
A scheme used to arrange organisms into groups called 'taxa'…
the branch of taxonomy concerned with the assignment of names…
the practical side of toxonomy, the process of determining if…
a nutrient substance in which selected microorganisms are cul…
Classification
A scheme used to arrange organisms into groups called 'taxa'…
Nomenclature
the branch of taxonomy concerned with the assignment of names…
26 terms
micr 221 Lab 1
Most common growth medias? Why?
Liquid media (broth) are not suitable…
What does solid media contain? How is…
What does an agar plate contain?
Nutrient broth and nutrient agar plates because they support…
Identification or isolation of bacterial species.
A solidifying agent such as agar, most often it is poured and…
It is a Petri dish that contains growth medium (typically aga…
Most common growth medias? Why?
Nutrient broth and nutrient agar plates because they support…
Liquid media (broth) are not suitable…
Identification or isolation of bacterial species.
83 terms
MICR221 Lab Test
What is Tryptic Soy Broth (TSB)?
What does TSB contain?
What is Tryptic Soy agar (TSA)?
What is subculturing and how is it use…
Nutrient broth - supports the growth of a wide range of MO. p…
Tryptone - pancreatic digest of casein and enzymatic digests…
General purpose nutrient agar with a neutral pH, and is frequ…
Maintain a pure culture when transferring microorganisms from…
What is Tryptic Soy Broth (TSB)?
Nutrient broth - supports the growth of a wide range of MO. p…
What does TSB contain?
Tryptone - pancreatic digest of casein and enzymatic digests…
26 terms
MICR221: Lab 4
Colony Forming Units per millilitre fo…
Mycology
Mycelium
What are the phylums of fungi?
number of colonies x 1/volume plated (ml) x dilution factor =…
Study of fungi
mass of intertwining threads
-Zygomycota... -Ascomycota... -Basidiomycota
Colony Forming Units per millilitre fo…
number of colonies x 1/volume plated (ml) x dilution factor =…
Mycology
Study of fungi
95 terms
MICR221- Lab Test
75ul
Media Types
Why use a solid media vs. broth?
Autoclave
0.075mL
Liquid (broth) and solid
allows bacteria to grow in colonies. suitable for the identif…
121C and 15psi for 15min
75ul
0.075mL
Media Types
Liquid (broth) and solid
MICR221 Viruses
Helical capsid virus
Isocahedral capsid virus
Complex capsid virus
Miscellaneous capsid virus
Tobacco mosaic virus, Influenza
Adenovirus, papilloma virus
T4 bacteriophage
Poxvirus
Helical capsid virus
Tobacco mosaic virus, Influenza
Isocahedral capsid virus
Adenovirus, papilloma virus
30 terms
MICR221 (8)
Bacterial Adherence - Significance
Non-pilus adhesins
Capsules
Where is the capsule formed? Activated…
Adherence to host cells/tissue - infection... Adherence to surfa…
Surface proteins (e.g. Helicobacter pylori - recognize sugars…
Numerous strands of polysaccharide extending up to 50 μm from…
in the cytoplasm, the cell membrane in carriers, the cell wall
Bacterial Adherence - Significance
Adherence to host cells/tissue - infection... Adherence to surfa…
Non-pilus adhesins
Surface proteins (e.g. Helicobacter pylori - recognize sugars…
84 terms
MICR221: Bacterial Powerhouse
What Polymers do bacteria grow on?
Protease
Cellulases
Lipases
Polysaccharide, Protein, Organic material, Cellulose and Starch
Peptide
Breaks down cellulose (B-1-4 linkage)
Fats and Glycerol
What Polymers do bacteria grow on?
Polysaccharide, Protein, Organic material, Cellulose and Starch
Protease
Peptide
MICR221 Medical
Pathogenesis
Parasitism
Example of mutualism and commensalism
Examples of bacteria:... Skin... Oral cavity…
Symbiotic relationship in which one organism harms the other.…
Symbiotic relationship in which one organism harms the other.…
Mutualism = bovine rumen... Bacteria provide nutrients (breakdow…
Skin: Streptococcus ssp and Staphylococcus ... Oral cavity: Stre…
Pathogenesis
Symbiotic relationship in which one organism harms the other.…
Parasitism
Symbiotic relationship in which one organism harms the other.…
58 terms
MICR 221 Virology 4
What are the 4 possible characteristic…
What are the 4 possible characteristic…
Ambi-sense
Monopartite
1. Linear or circular ... 2. Single stranded or double stranded…
1. Linear or circular... 2. Single stranded or double stranded... 3…
RNA viruses that are both (+) and (-) polarity
single segment
What are the 4 possible characteristic…
1. Linear or circular ... 2. Single stranded or double stranded…
What are the 4 possible characteristic…
1. Linear or circular... 2. Single stranded or double stranded... 3…
MICR221 Microbial Growth
Mean generation time
Binary fission
Surface:volume ratio
Physical growth requirements
Time taken for one cell to divide
Bacterial cell division.... Cell elongates and chromosomes repli…
Benefits being a small microorganism... Larger surface:volume ra…
Gaseous atmospher... pH... Temperature... Osmotic pressure
Mean generation time
Time taken for one cell to divide
Binary fission
Bacterial cell division.... Cell elongates and chromosomes repli…
17 terms
MICR221 Lec8
what are two possible consequences hap…
what is the structure of endospore?
What are the functions provided by the…
What are the functions provided by the…
1, some bacteria can go metabolically dormant stage (state of…
Core, cortex, coat, exosporium
It contains 15% of water which makes it jelly cause a heat re…
contains SASPs (small acid-soluble proteins) which bind to DN…
what are two possible consequences hap…
1, some bacteria can go metabolically dormant stage (state of…
what is the structure of endospore?
Core, cortex, coat, exosporium
22 terms
MICR221 Lec10
What's disinfectants?
What's antiseptics?
What's preservatives?
How is the population size influencing…
are chemicals that are capable of killing microorganisms.... Can…
are chemical agents that kill or inhibit growth of microorgan…
are included in pharmaceutical preparations to prevent microb…
the time it takes to achieve sterility, or to reduce microbia…
What's disinfectants?
are chemicals that are capable of killing microorganisms.... Can…
What's antiseptics?
are chemical agents that kill or inhibit growth of microorgan…
12 terms
MICR221 Lecture 7
Do not know the exact chemical composi…
Defined media are used to culture ____…
What are the 5 types of specialised me…
Transport Media
Complex media
fastidious, chemically pure, sugars, vitamins, amino acids
Transport media, Enriched media, Enrichment broth, selective…
-Used for temporary storage of microbes during transport... -Mai…
Do not know the exact chemical composi…
Complex media
Defined media are used to culture ____…
fastidious, chemically pure, sugars, vitamins, amino acids
143 terms
MICR 221: Microbial Growth and Control
Binary fission
Mean generation time
What is mean generation time of E.coli…
Cocci
Occurs in bacteria and archea these are haploid. The cell wou…
The time it takes for one cell (and therefore the whole popul…
E.coli: 20 minutes... M. Tuberculosis: 24 hours
rough spherical cells
Binary fission
Occurs in bacteria and archea these are haploid. The cell wou…
Mean generation time
The time it takes for one cell (and therefore the whole popul…
42 terms
MICR221 Lec7
What's complex media?
what sorts of complex sources found in…
What's defined media?
What are the main 6 specialised medias?
they are chemically not pure (contains some gradients of unkn…
-Glucose (provide carbon and energy)... -Beef extract (provide…
they are chemically pure (contains only known gradients)
Transport media, Enriched media, Enrichment broths, Selective…
What's complex media?
they are chemically not pure (contains some gradients of unkn…
what sorts of complex sources found in…
-Glucose (provide carbon and energy)... -Beef extract (provide…
21 terms
MICR221 Lecture 6
What is the mechanism of bacterial rep…
How does Binary fission occur?
What does binary fission result in?
What is the "mean generation time"?
Binary fission
1. The cell elongates and DNA replicates... 2.Chromosomes start…
two daughter cells which are exact replicates of the parent c…
the time it takes form the whole population to divide
What is the mechanism of bacterial rep…
Binary fission
How does Binary fission occur?
1. The cell elongates and DNA replicates... 2.Chromosomes start…
175 terms
MICR 221 Quiz #1
Where does Epulopiscium fishelonsii re…
what is unique about the genome of Epu…
What is a benefit of large size for ba…
What percentage of bacteria is non-cul…
In the gut of Brown Surgeonfish (symbiont)
It is a polyploid (>100,00 copies)... An adaptation to its larg…
avoid predation
95%
Where does Epulopiscium fishelonsii re…
In the gut of Brown Surgeonfish (symbiont)
what is unique about the genome of Epu…
It is a polyploid (>100,00 copies)... An adaptation to its larg…
27 terms
MICR221 Lec11
what are pathogens?
what are main characteristics of comme…
what's symbiosis?
what are 4 main types of symbiosis?
bacteria that cause diease
-live in the human body, but do not cause disease ... -benefit…
living together of two organisms
commensalism, mutualism, pathogenesis and parasitism
what are pathogens?
bacteria that cause diease
what are main characteristics of comme…
-live in the human body, but do not cause disease ... -benefit…
32 terms
MICR221 Lecture 8
Of the small group of bacteria than ca…
When does endospore formation occur?
Why is endospore formation not a repro…
How long does sporulation take to occu…
positive
When environmental conditions become unfavourable, such as if…
Because it does not result in two daughter cells identical to…
sporulation - 8 hours... germination - 15 minutes
Of the small group of bacteria than ca…
positive
When does endospore formation occur?
When environmental conditions become unfavourable, such as if…
21 terms
MICR221 Viruses 1
Adenoviridae
Asfarviridae
Baculoviridae
Coronaviridae
dsDNA, linear genome - pseudocircular, icosahedral, non-envel…
dsDNA, linear, large DNA virus, icosahedral, enveloped, budding
dsDNA, circular, filamentous, enveloped, gp64
+ssRNA, helical, enveloped
Adenoviridae
dsDNA, linear genome - pseudocircular, icosahedral, non-envel…
Asfarviridae
dsDNA, linear, large DNA virus, icosahedral, enveloped, budding
270 terms
MICR 221 Quiz #2
What are the 2 types of cytoplasmic me…
What are the 4 types of cell walls?
What are 2 components of the CM?
What molecules make up the lipid bilay…
-Eubacterial... -Archael
-Gram negative... -Gram positive... -Mycobacterial... -Archael
-lipid bilayer... -proteins
glycerol fatty acid-based lipids
What are the 2 types of cytoplasmic me…
-Eubacterial... -Archael
What are the 4 types of cell walls?
-Gram negative... -Gram positive... -Mycobacterial... -Archael
8 terms
MICR221 Aseptic Techniques
What two main material are sterilised…
The wire loop should be left in the fl…
What happens to the heat when sterilis…
For some objects such as forceps, spre…
Wire loops and bottles
It glows red to insure infectious agents and inactivated
Creates convection currents out of bottle as well as sterilis…
Dip in ethanol
What two main material are sterilised…
Wire loops and bottles
The wire loop should be left in the fl…
It glows red to insure infectious agents and inactivated
MICR221 Virology
Acellular
Bacteria proof (paper)
Toxin
Ribonucleoprotein
Viruses are ______ microorganisms, which require host cell ma…
Friedrich Loeffler and Paul Frosch (Germany) used this to mak…
Martinus Beijerinck found that the agent (virus) could reprod…
In 1936, Frederick C. Bawden (Britain) demonstrated that viru…
Acellular
Viruses are ______ microorganisms, which require host cell ma…
Bacteria proof (paper)
Friedrich Loeffler and Paul Frosch (Germany) used this to mak…
25 terms
MICR 221 terms
Psychrophile
Psychrotrophs
Mesophiles
Thermophiles
Prefer −5-20
Prefer -2-35
Prefer 15-45
Prefer 40-80
Psychrophile
Prefer −5-20
Psychrotrophs
Prefer -2-35
117 terms
MICR 221 lab test
What is the typical method of steriliz…
How is tryptic soy broth made?
How is tryptic soy agar made?
List the ingredients and their quantit…
Autoclaving (121 degrees for 15 min)
By adding deionised or distilled water to the dried media com…
By adding 15 g of agar to 1000ml of TSB
Tryptone 17g... Enzymatic digest of soybean meal 3g... Sodium chlor…
What is the typical method of steriliz…
Autoclaving (121 degrees for 15 min)
How is tryptic soy broth made?
By adding deionised or distilled water to the dried media com…
27 terms
MICR 221 LEC_004
DNA Viruses
RNA virus
Hepatitis Deltavirus
+ RNA/DNA
-Linear Or Circular... - ss or ds... - ss can be + or -... - Partially…
- linear or circular (only Hepatitis Deltavirus Circular) ... -…
- Only circular RNA virus
- + strand... - Immediately translatable info... - All mRNA... - not a…
DNA Viruses
-Linear Or Circular... - ss or ds... - ss can be + or -... - Partially…
RNA virus
- linear or circular (only Hepatitis Deltavirus Circular) ... -…
26 terms
MICR 221 Exam
Poliovirus and rhinovirus receptor
Adenovirus receptor
Influenza receptor
Retrovirus receptor
Canyon at bottom of depression accessible by receptor but not…
Fibre extending from penton base
Hemagglutinin and neuraminidase spikes of envelope
Interaction of HIV glycoproteins with cell surface receptors
Poliovirus and rhinovirus receptor
Canyon at bottom of depression accessible by receptor but not…
Adenovirus receptor
Fibre extending from penton base
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