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Study sets matching "clinical microbiology profile"

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Study sets matching "clinical microbiology profile"

34 terms
Microbiology Profile
Acute
Bacteremia
Carrier
Colonization
the disease symptoms develop rapidly
when bacteria are found in the blood
a human vector
a superficial population of reproducing microbes
Acute
the disease symptoms develop rapidly
Bacteremia
when bacteria are found in the blood
80 terms
clinical microbiology
pasteurization
aseptic transfer
steam sterilization
sterilization by filtration
the process of treating a substance with heat to destroy or s…
Process of adding living microbes to media without contaminat…
The most common method of sterilizing instruments
-Bacteria are physically removed via specialized membrane fil…
pasteurization
the process of treating a substance with heat to destroy or s…
aseptic transfer
Process of adding living microbes to media without contaminat…
20 terms
Clinical Microscopy profile
Urinary cast algorhythm
Acute glomerulonephritis
Dysmorphic RBC
Dysmorphic RBC
"Urinary cast"
RBC cast
Acute glomerulonephritis... DDx. IgA nephropathy... Lupus nephriti…
Identify urinary sediment
Urinary cast algorhythm
"Urinary cast"
Acute glomerulonephritis
RBC cast
24 terms
Clinical Microbiology Chapter 7
Purulent
Wet Prep (Wet Mount)
Simple Stain
Differential Stain
Consisting of matter or pus; resembling pus
A glass slide holding a specimen suspended in a drop of liqui…
Staining procedure that uses one stain
Allow determination of cell size, morphology, and arrangement…
Purulent
Consisting of matter or pus; resembling pus
Wet Prep (Wet Mount)
A glass slide holding a specimen suspended in a drop of liqui…
85 terms
Clinical Microbiology Chapter 1
Acid Fast
Aerotolerant anaerobes
Archaeobacteria
Bacteria
Used to visualize mycobacteria; mycobacteria have mycoic acid…
Do not utilize oxygen but can survive and grow in its presence
Bacteria that lives in extreme conditions (high and low temp…
A member of a large group of unicellular microorganisms lacki…
Acid Fast
Used to visualize mycobacteria; mycobacteria have mycoic acid…
Aerotolerant anaerobes
Do not utilize oxygen but can survive and grow in its presence
50 terms
Clinical Profile-Module 4
Indications
Hypertension
Why Reducing Blood Pressure is so Impo…
Recommended Starting Dose
-Bystolic is a beta blocker that is indicated ONLY for the tr…
Control of high blood pressure should be part of comprehensiv…
It has been shown in randomized controlled trials that is the…
For most patients, the recommended starting dose is 5mg once…
Indications
-Bystolic is a beta blocker that is indicated ONLY for the tr…
Hypertension
Control of high blood pressure should be part of comprehensiv…
103 terms
Clinical Microbiology
What are the common types of bacteria…
Commensalism
Mutualism
Parasitism
Microorganisms benefits while host is not harmed.
Microorganism and host benefit from eachother
Microorganisms that benefit while the host is harmed.
What are the common types of bacteria…
Commensalism
Microorganisms benefits while host is not harmed.
100 terms
Clinical Microbiology*
What is Microbiology?
What is Mycology?
What type of parasites are viruses?
What is Virulence?
The study of biological organisms too small to be seen with t…
The study of simple plants know as fungi and yeast; Kingdom F…
Obligate intracellular parasites
The potential of a pathogen to cause disease
What is Microbiology?
The study of biological organisms too small to be seen with t…
What is Mycology?
The study of simple plants know as fungi and yeast; Kingdom F…
103 terms
clinical microbiology
streptococcus catalase test
streptococcus gram stain
streptococcus microscopically look like
streptococcus air conditions
negative
positive
cocci singly, pairs or chains
facultatively anaerobic
streptococcus catalase test
negative
streptococcus gram stain
positive
20 terms
Emergence Profile and Clinical Practice
biological contours
where are facial and lingual convexiti…
how large is the facial and lingual co…
where are mesial and distal concavitie…
-facial and lingual convexities... -mesial and distal concavitie…
near CEJ
1/2 mm
coming out of gingiva
biological contours
-facial and lingual convexities... -mesial and distal concavitie…
where are facial and lingual convexiti…
near CEJ
clinical microbiology
Gram stain procedure
diagnostic cycle
shapes
hemolytic reactions
know steps, dont worry about times... can use direct specimen o…
staph. - clusters of cocci... strep - cocci in chains... Spiroche…
alpha - partial hemolysis... Beta - total hemolysis (pictured h…
Gram stain procedure
know steps, dont worry about times... can use direct specimen o…
diagnostic cycle
32 terms
Clinical Microbiology Chapter 10
Active immunity
Passive immunity
Natural immunity
Artificial immunity
Immunity that occurs when a person's own immune system produc…
Immunity conferred by transferring antibodies from an individ…
The immunity you inherit as a result of the genes you have th…
Immunity acquired as a result of deliberate exposure to antig…
Active immunity
Immunity that occurs when a person's own immune system produc…
Passive immunity
Immunity conferred by transferring antibodies from an individ…
12 terms
Profiling 04 (Clinical)
Clinical profiling often relies on...
Copson et al. 2006 said the advice giv…
Reflexive
Turco (1990) 4 Step Model
the judgement of experts
Custom made... Interactive... Reflexive... Subjective
If new info arives, must be able to be changed
1. Look at crime as a whole... 2. Assess crime scene (look for e…
Clinical profiling often relies on...
the judgement of experts
Copson et al. 2006 said the advice giv…
Custom made... Interactive... Reflexive... Subjective
59 terms
Clinical microbiology
Aerobic
Anaerobic
Antibiotic susceptibility testing
Bacillus
Requiring oxygen for growth
Growing in the absence of oxygen
Determining the susceptibility of bacteria to specific antibi…
A rod-shaped bacterium
Aerobic
Requiring oxygen for growth
Anaerobic
Growing in the absence of oxygen
50 terms
Clinical Microbiology
non-pathogens or saprophytes
pathogens
opportunists
the importance of identification
harmless microorganisms that colonize the human body
agents of disease
microbes that cause disease in only favorable conditions
distinguishing pathogens from harmless microbes, characterizi…
non-pathogens or saprophytes
harmless microorganisms that colonize the human body
pathogens
agents of disease
170 terms
Clinical Microbiology
How is the derm a natural defense?
Name defensive reflexes related to the…
Other natural defenses include:
T or F: Acquired immune system is invo…
It is keratinized and waterproof.
BELL = cornea moves out of the way when projectile is thrown…
Cilia ... Tears... Local chemical factors and normal flora... Immune s…
False. Often acquired immune system is NOT involved with acut…
How is the derm a natural defense?
It is keratinized and waterproof.
Name defensive reflexes related to the…
BELL = cornea moves out of the way when projectile is thrown…
215 terms
Clinical Laboratory Science: Microbiology
Gram Stain
Polymorphonuclear Neutrophil (PMN)
Lymphocytes
Macrophages
* Stain to detect bacteria & yeast... 1. CRYSTAL VIOLET - 1min... 2…
* Segmented nucleus... * Attracts infected site in response to b…
* Smaller than PMNs... * Round nucleus with thin border of cytop…
* Slightly larger than PMNs... * Large, slightly indented, non-s…
Gram Stain
* Stain to detect bacteria & yeast... 1. CRYSTAL VIOLET - 1min... 2…
Polymorphonuclear Neutrophil (PMN)
* Segmented nucleus... * Attracts infected site in response to b…
45 terms
Clinical Microbiology Chapter 2
Carrier
Commensalism
Disease
Indigenous flora
An individual that has one copy of a recessive allele that ca…
A relationship between two organisms in which one organism be…
An interruption, cessation, or disorder of a body, system, or…
Bacteria that is found on or in body sites of healthy persons
Carrier
An individual that has one copy of a recessive allele that ca…
Commensalism
A relationship between two organisms in which one organism be…
20 terms
Clinical Microbiology
Louis Pasteur
John Tyndall
Francesco Redi
John Needham
"Father of Microbiology"; filtered air through cotton plug an…
Various broths require different boiling time.... There are two…
Didn't believe in Spontaneous Generation. He demonstrated wor…
Supported Spontaneous Generation Theory; he boiled various br…
Louis Pasteur
"Father of Microbiology"; filtered air through cotton plug an…
John Tyndall
Various broths require different boiling time.... There are two…
37 terms
Byvalson- Clinical Profile Module 5
Meta-Analysis on Reducing Blood Pressure
Beta Blockers
Cardiovascular Clinical Use of Beta Bl…
Mechanism of Action: Beta Blockers
123 studies revealed that every 10mmHg reduction in systolic…
Beta blockers may be a preferred agent for patients with hype…
Many beta blockers are used to treat a number of cardiovascul…
In general, beta blockers bind to beta adrenoreceptors in the…
Meta-Analysis on Reducing Blood Pressure
123 studies revealed that every 10mmHg reduction in systolic…
Beta Blockers
Beta blockers may be a preferred agent for patients with hype…
26 terms
Pathogen profiles microbiology
Bacillus anthracis
Clostridium perfringens, clostridium t…
Clostridium difficile
Listeria monocytogenes
Large gram positive bacilli. Long chain of block shaped with…
Gram positive bacilli single or pairs with endospores. Strict…
Gram positive bacilli single or short chains. Endospores are…
Gram positive bacilli in single or short chains. Identified b…
Bacillus anthracis
Large gram positive bacilli. Long chain of block shaped with…
Clostridium perfringens, clostridium t…
Gram positive bacilli single or pairs with endospores. Strict…
47 terms
Clinical Microbiology
what is the purpose of clinical microb…
what two things help assist physicians…
methods used in microbiology
what is the fastest method
assist physicians in the diagnosis and treatment of infectiou…
-determine the etiology of infections... -determine the suscepti…
-culture... -direct detection... -serology
direct detection
what is the purpose of clinical microb…
assist physicians in the diagnosis and treatment of infectiou…
what two things help assist physicians…
-determine the etiology of infections... -determine the suscepti…
86 terms
Clinical Microbiology
1-20 Taxonomy
Examples of Prokaryotes are ___
Examples of Eukaryotes are ___
Bacterial Cell Wall contains ___
...
Bacteria
Parasites and fungi
Peptidoglycan (murein layer). Gives cells their shape.
1-20 Taxonomy
...
Examples of Prokaryotes are ___
Bacteria
30 terms
Clinical microbiology
Blood agar plate
Brucella blood agar plate
MacConkey's agar plate
Hektoen enteric agar
Primary isolation medium, streak for colony isolation
Primary isolation medium, streak for colony iaolation, provid…
Primary isolation medium for selection of gram-negative organ…
Used for direct isolation of Salmonella spp from feces, ferme…
Blood agar plate
Primary isolation medium, streak for colony isolation
Brucella blood agar plate
Primary isolation medium, streak for colony iaolation, provid…
89 terms
Clinical Microbiology
oxidase
tube indole
catalase
phenylalanine
tetramethylphenylene diamine
paradimethylaminobenzaldehyde
H2O2
10% ferric chloride
oxidase
tetramethylphenylene diamine
tube indole
paradimethylaminobenzaldehyde
15 terms
Clinical microbiology
Oral Cavity
Respiratory Tract
Lower GI tracts
Skin
Normal human microbiota:... Viridans group streptococci... Actinomy…
Normal human microbiota:... Streptococci... Staphylococci... Neisseria…
Normal human microbiota:... Bacteroides... Clostridium... Enterobacter…
P. acnes... Staphylococci
Oral Cavity
Normal human microbiota:... Viridans group streptococci... Actinomy…
Respiratory Tract
Normal human microbiota:... Streptococci... Staphylococci... Neisseria…
10 terms
Clinical Microbiology
Methods
Specimens
Handling/Transport
Microscopy
-Used to diagnose infectious disease: Direct detection, Culture
-# of specimens used... -Swabs (tend to use transport system), n…
-Lab needs a quality specimen: proper labeling, rapid transpo…
-Stains... -Stat gram stain (not very sensitive, may not see som…
Methods
-Used to diagnose infectious disease: Direct detection, Culture
Specimens
-# of specimens used... -Swabs (tend to use transport system), n…
82 terms
Clinical Microbiology
7 cultures that can be taken
Approximate time to identify organism
_____, ______, and _______ are specime…
Specimen should be collected _________…
1. blood... 2. spinal fluid... 3. sputum... 4. joint fluid... 5. urine... 6.…
24-48 hours
tissues, aspirates, and fluids are specimens of choice
prior to administration of abx. May get a false negative.
7 cultures that can be taken
1. blood... 2. spinal fluid... 3. sputum... 4. joint fluid... 5. urine... 6.…
Approximate time to identify organism
24-48 hours
16 terms
Clinical Pharmacy #1: Chemistry Profile
FBS
BUN
UREA
BLOOD URIC ACID
measures blood sugar level
measure waste in blood
estimating filtering capacity of kidney
created during the breakdown of purine
FBS
measures blood sugar level
BUN
measure waste in blood
68 terms
clinical microbiology
what you can use swabs for
what kind of swab to use
transport media
specimen containers
upper respiratory tract, external ear, eye, genital tract, sk…
NOT cotton bc excessive fatty acids which are toxic to some b…
keeps bacteria alive in tube... should be provided with swab col…
clean and sterile ... use when can't swab ie urine
what you can use swabs for
upper respiratory tract, external ear, eye, genital tract, sk…
what kind of swab to use
NOT cotton bc excessive fatty acids which are toxic to some b…
10 terms
Clinical Microbiology
preliminary examination of bacteria
a gram stain with no organisms seen ru…
number of organisms/ml of pus required…
bacteria culture
direct examination/ wet preparation
a gram stain with no organisms does not rule out an infection
100,00 organisms/ml of pus
blood agar grows most bacteria... bacteria grow aerobically, ana…
preliminary examination of bacteria
direct examination/ wet preparation
a gram stain with no organisms seen ru…
a gram stain with no organisms does not rule out an infection
36 terms
Clinical Microbiology
PTG Structure
Enzyme that catalyzes cross-linking
Mechanism of penicillin
Components of gm+ cell wall
disaccharide + chain of 4 aa units that covalently bind to ot…
transpeptidases (penicillin-binding proteins);
blocks cross-linking (cannot affect gram - organisms, as LPS…
2 layers of cell wall: 1. PTG layer (thick, complex), 2. inne…
PTG Structure
disaccharide + chain of 4 aa units that covalently bind to ot…
Enzyme that catalyzes cross-linking
transpeptidases (penicillin-binding proteins);
35 terms
clinical microbiology
Based on gram stain results and morpho…
most common pathogen for strep throat
most common pathogen for whooping cough
most common pathogen for scarlet fever
Gram + cocci in clusters = Staphylococci... Gram + cocci in pai…
Group A strep pyogenes
Bordetella pertussis
Group A streptococcus pyogenes, organisms produces pyrogenic…
Based on gram stain results and morpho…
Gram + cocci in clusters = Staphylococci... Gram + cocci in pai…
most common pathogen for strep throat
Group A strep pyogenes
Clinical Microbiology
Impetigo, Rheumatic fever, glomerulone…
Impetigo, Osteomyelitis, scaled skin s…
Lyme disease, Bell's palsy
Herpes zoster, or Chickenpox
strep pyogenes
Strep aureus
Borrelia Burgdorferi
Varicella-zoster virus
Impetigo, Rheumatic fever, glomerulone…
strep pyogenes
Impetigo, Osteomyelitis, scaled skin s…
Strep aureus
30 terms
Clinical Microbiology
gram positive bacteria
gram negative bacteria
cocci shape
bacilli shape
peptidoglycan staining blue from cystal violet.... two layered m…
LPS stained with safranin to appear red.... 3 layers of membrane…
spherical
rods
gram positive bacteria
peptidoglycan staining blue from cystal violet.... two layered m…
gram negative bacteria
LPS stained with safranin to appear red.... 3 layers of membrane…
118 terms
Clinical Microbiology
Laboratory Work-flow
In-patient Microbiology Non-serology R…
Accessioning
Processing
1. Collection 2. Transport/storage 3. Accessioning 4. Process…
1. Physicians name/contact info 2. Specimen type (induce meth…
Process of identifying the specimen- properly labeled, proper…
Tests, media type and additional processing requirements and…
Laboratory Work-flow
1. Collection 2. Transport/storage 3. Accessioning 4. Process…
In-patient Microbiology Non-serology R…
1. Physicians name/contact info 2. Specimen type (induce meth…
48 terms
Clinical Microbiology
Clinical Microbiology
Infectious Disease
communicable disease
class one
branch of micro bio dealing with the spread of infections and…
disease caused by some type of micro organism that can invade…
contagious disease, disease that can be spread from one host…
those that are never reported to health officials ... ex: common…
Clinical Microbiology
branch of micro bio dealing with the spread of infections and…
Infectious Disease
disease caused by some type of micro organism that can invade…
69 terms
Clinical Microbiology
Chocolate agar with factors V (NAD⁺) a…
Thayer-Martin (or VPN) media - Vanco i…
Bordet-Gengou (potato) agar
Cystine-Tellurite Plage, Loffler's media
H. Influenzae
Neisseria Gonorrhoeae
Bordetella Pertussis
C. Diphtheriae
Chocolate agar with factors V (NAD⁺) a…
H. Influenzae
Thayer-Martin (or VPN) media - Vanco i…
Neisseria Gonorrhoeae
18 terms
Clinical Microbiology
Sequelae
CSF and sputum
Selective media and identification of…
Differential media and identification…
Condition that is the consequence of a previous disease or in…
Specimens from here can be directly stained with gram-stain p…
Removes non-pathogens and allows the growth of the pathogen,…
Exploits pathogens unique biochemical properties. Strains gro…
Sequelae
Condition that is the consequence of a previous disease or in…
CSF and sputum
Specimens from here can be directly stained with gram-stain p…
15 terms
Clinical Microbiology
Traditional microbiology
major difference between traditional a…
what can mass spectrometry been done on?
What do you need for PCR?
microscopy, stains, culture, antigen detection, serology
new is faster. (traditional takes 48-72h, new takes minutes)
pure colony growth of bacteria or yeast
template DNA, Taq polymerase (DNA polymerase), 2 primers, DNA…
Traditional microbiology
microscopy, stains, culture, antigen detection, serology
major difference between traditional a…
new is faster. (traditional takes 48-72h, new takes minutes)
37 terms
Clinical Microbiology
Diagnosing aetiology
Demonstrating organisms & products
Isolate the organism
Demonstrate serological response
demonstrate organism, component or product... isolate & identify…
microscopy... latex agglutination ... solid phase assay... capture ass…
media... traditional: morphology, biochemistry... molecular: nuclei…
Ab response = tube agglutination ... Ab detection with solid pha…
Diagnosing aetiology
demonstrate organism, component or product... isolate & identify…
Demonstrating organisms & products
microscopy... latex agglutination ... solid phase assay... capture ass…
36 terms
MICROBIOLOGY: PROKARYOTIC PROFILES
WHICH OF THE FOLLOWING IS NOT FOUND IN…
PILI ARE TUBULAR SHAFTS IN ______BACTE…
AN EXAMPLE OF A GLYCOCALYX IS
WHICH OF THE FOLLOWING IS A PRIMARY BA…
ACTIN CYTOSKELETON
GRAM-NEGATIVE, GENETIC EXCHANGE
A CAPSULE
SUPPORT
WHICH OF THE FOLLOWING IS NOT FOUND IN…
ACTIN CYTOSKELETON
PILI ARE TUBULAR SHAFTS IN ______BACTE…
GRAM-NEGATIVE, GENETIC EXCHANGE
Clinical Microbiology
Clinical and Diagnostic Microbiology i…
Labs can usually isolate, ID, and dete…
If genetic techniques are introduced,…
Why is it bad to prescribe 'broad spec…
isolation, identification
48
a few
Selects for antibiotic resistance
Clinical and Diagnostic Microbiology i…
isolation, identification
Labs can usually isolate, ID, and dete…
48
33 terms
Microbiology: Clinical Microbiology and Immunology
Clinical microbiologist
Direct Immunofluorescence
Ribotyping
Multilocus sequence typing (MLST)
Isolate and rapidly identify pathogenic microorganisms from c…
involves fixing the specimen containing the antigen of intere…
Classification of bacteria based on the high level of 16S rRN…
technique where "housekeeping" genes, instead of 16S rRNA gen…
Clinical microbiologist
Isolate and rapidly identify pathogenic microorganisms from c…
Direct Immunofluorescence
involves fixing the specimen containing the antigen of intere…
9 terms
Clinical Microbiology
Which of the following are FALSE conce…
Which of the following represents the…
Consider organisms that are eukaryotic…
Which of the following matches the org…
c)The primary role of microbes on the planet involves causing…
a)Staphylococcus aureus (italicized)
b)fungi
a)eukarya--single-celled and multicellular organisms, with a…
Which of the following are FALSE conce…
c)The primary role of microbes on the planet involves causing…
Which of the following represents the…
a)Staphylococcus aureus (italicized)
16 terms
Clinical Microbiology
What forms of staining are we worrying…
What color are Gm+ and Gm- bacteria af…
What are the 2 major shapes of bacteria?
What layer of the bacteria is stained?
Gram stain... Acid-fast stain
Gm+ = purple/blue... Gm- = pink/red
Coccus/Cocci... Bacillus/Bacilli
Peptidoglycan
What forms of staining are we worrying…
Gram stain... Acid-fast stain
What color are Gm+ and Gm- bacteria af…
Gm+ = purple/blue... Gm- = pink/red
85 terms
Clinical Microbiology
What are the two types of Fungi?
What is a yeast?
What is binary fission?
What is budding?
Yeasts and Molds
single celled Eukaryote that divides by binary fission or bud…
After replicating its genetic material, the cell divides into…
a method of asexual reproduction. With budding, a new organis…
What are the two types of Fungi?
Yeasts and Molds
What is a yeast?
single celled Eukaryote that divides by binary fission or bud…
12 terms
clinical microbiology
specimen collection
example of specimen collection
what is done at the lab
examples of procedures at the lab
aseptic techniques, correct location, proper protocol, transp…
specimen sent to lab, in sealed bag separated from workup.
microscopy, culturing, biochemical tests, dna probes, pcr, fl…
specimen is plated on various medias
specimen collection
aseptic techniques, correct location, proper protocol, transp…
example of specimen collection
specimen sent to lab, in sealed bag separated from workup.
20 terms
Clinical microbiology
3 normal bacteria present that can tur…
another 2 less common infections in CF?
What is the viscous circle hypothesis?
What are the infection steps of psuedo…
Pseudomonas aeruginosa... staphyllococcus aereus ... Haemophilus in…
bukholderia capacia... mycobacterium abscessus
infection -> inflammation -> infection
acquisition -> attachment ->colonies -> chronic infection ->…
3 normal bacteria present that can tur…
Pseudomonas aeruginosa... staphyllococcus aereus ... Haemophilus in…
another 2 less common infections in CF?
bukholderia capacia... mycobacterium abscessus
34 terms
Clinical Microbiology
Which organisms of human diseases are…
Which cell type has a single circular…
T/F Eukaryotes contain organelles like…
Prokaryotes have a cell wall on the ex…
c. Fungi (yeasts/molds)... d. Parasites (protozoa/helminths)
Prokaryotes (bacteria)
True
Peptidoglycans (polymer of amino acids and sugars)
Which organisms of human diseases are…
c. Fungi (yeasts/molds)... d. Parasites (protozoa/helminths)
Which cell type has a single circular…
Prokaryotes (bacteria)
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