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Study sets matching "cloning analysis"

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Study sets matching "cloning analysis"

68 terms
Analysis of the Genome (Cloning)
A group of cells that are all descenda…
What are the three approaches to cloni…
A long-lived collection of cellular cl…
__________include an origin for DNA re…
Cellular Clone... DNA Clone
1) Genomic library-Collection of cloned genomic DNA fragments…
Genomic Library
Vectors
A group of cells that are all descenda…
Cellular Clone... DNA Clone
What are the three approaches to cloni…
1) Genomic library-Collection of cloned genomic DNA fragments…
36 terms
Cloning and Molecular Analysis of Genes
DNA can be undergo replication. DNA tr…
DNA polymerase, DNA ligase, Reverse tr…
Restriction enzyme
Methylase
Central dogma of Molecular Genetics
Enzymes for DNA analysis and molecular cloning
cleave DNA in a sequence specific manner.
DNA methylation involved the addition of a methyl group to DNA.
DNA can be undergo replication. DNA tr…
Central dogma of Molecular Genetics
DNA polymerase, DNA ligase, Reverse tr…
Enzymes for DNA analysis and molecular cloning
DNA cloning/analysis
A gene
Coding regions
Gene components
Simple eukaryotic transcription unit
Entire DNA sequence required for synthesis of a functional pr…
Exon
Exons, control regions, introns
Single monocistronic mRNA
A gene
Entire DNA sequence required for synthesis of a functional pr…
Coding regions
Exon
16 terms
Clone Isolation/Analysis & Cancer
DNA Probes
Southern Blot Analysis
Polymerase Chain Reaction
Sanger Sequencing
Short single-stranded stretches of DNA of known composition.…
Used to show the location of a gene within a larger fragment…
PCR - A fast inexpensive method of replicating a DNA sequence…
Reveals the order of base pairs in an isolated DNA molecule.…
DNA Probes
Short single-stranded stretches of DNA of known composition.…
Southern Blot Analysis
Used to show the location of a gene within a larger fragment…
26 terms
Chapter 7: Cloning Genes for Analysis
Chimera
Cloning vecotr
Vector
ColE1 plasmid
Hybrid molecule of DNA that has DNA form more than one source…
Any molecule of DNA that can replicate itself inside a cell a…
In molecular biology, a vector is a molecule of DNA which can…
Small multicopy plasmid of Escherichia coli that forms the ba…
Chimera
Hybrid molecule of DNA that has DNA form more than one source…
Cloning vecotr
Any molecule of DNA that can replicate itself inside a cell a…
9 terms
PCR, Gene Cloning, DNA/Protein Purification and Analysis
What do you use as a cloning vector?
plasmids
What does a plasmid contain?
origin of replication
plasmids
self-replicating, circular DNA molecules found in bacteria
A typical plasmid includes an origin of replication site, mul…
where DNA replication begins (effective vs. ineffective sites
What do you use as a cloning vector?
plasmids
plasmids
self-replicating, circular DNA molecules found in bacteria
12 terms
Lecture 7 - Cloning actin gene in chicken - northern blot analysis
Protocol when cloning eukaryotic gene?
What is the next step following obtain…
Assessing tissue specific gene express…
Assessing developmental gene expression?
Perform the standard cloning steps with parallel gel enrichme…
Performing restriction enzyme mapping of positive clones and…
Isolate RNA from various organs / tissue types -> perform nor…
Take various time points of embryo & adult development RNA ti…
Protocol when cloning eukaryotic gene?
Perform the standard cloning steps with parallel gel enrichme…
What is the next step following obtain…
Performing restriction enzyme mapping of positive clones and…
10 terms
Genetics and Biotechnology: Cloning Section 5 and Human DNA Analysis Section 6
Clone
DNA Fingerprinting
Gene Therapy
D. all of the above
member of a population of genetically identical organisms pro…
analysis of sections of DNA that have little or no known func…
an absent or faulty gene is replaced by a normal, working gene
Genetic engineering involves... A. reading a DNA sequence... B. edi…
Clone
member of a population of genetically identical organisms pro…
DNA Fingerprinting
analysis of sections of DNA that have little or no known func…
8 terms
BIO 326 - Genetics: Gene Cloning; DNA Sequencing; Genomics and Sequence Analysis
Bioinformatics
Contigs
Dideoxynucleotide triphosphate
DNA sequencing
application of computer-bases information technology to study…
overlapping, continguous DNA sequences
chain terminating nucleotide
create recombinant DNA
Bioinformatics
application of computer-bases information technology to study…
Contigs
overlapping, continguous DNA sequences
7 terms
Lecture 6: (16S PCR, Phylogeny analysis, Gene clone library, massive parallel DNA sequencing, etc.)
16S RNA
Phylogeny analysis
16S rRNA gene clone library
Massively parallel DNA sequencing
- A "mosaic" of regions that are highly conserved among all b…
- Inferred evolutionary relationships between bacterial seque…
Advantages:... - 16S genes are large enough (~1.5 kb) to contain…
- aka "NextGen" or "high-throughput" sequencing... - Can be quan…
16S RNA
- A "mosaic" of regions that are highly conserved among all b…
Phylogeny analysis
- Inferred evolutionary relationships between bacterial seque…
26 terms
Cloning
cloning
reproductive cloning
therapeutic cloning
recombinant DNA technology
making copies of genes, animals, cells
whole animal cloning
cloning stem cells
A series of procedures that are used to join together (recomb…
cloning
making copies of genes, animals, cells
reproductive cloning
whole animal cloning
14 terms
Cloning
why clone genes
two ways to make gene copies
things you need to clone in bacteria
restriction enzymes
it's easier to manipulate cloned genes: to study genes/gene p…
1. insert a gene into a vector and express it in bacteria... 2.…
plasmid vector, gene of interest, DNA ligase, and restriction…
cut DNA at a recognition sequence
why clone genes
it's easier to manipulate cloned genes: to study genes/gene p…
two ways to make gene copies
1. insert a gene into a vector and express it in bacteria... 2.…
21 terms
Gene Mapping and Cloning
Linkage analysis
Halotypes
Syntenic
Mapping by recombination
locates genes on regions of chromosomes and determines which…
combo of alleles on a chromosome... more likely to inherit A1B1…
genes on same chromosome
chromosomes reorganize at meiosis... multiple crossovers can occ…
Linkage analysis
locates genes on regions of chromosomes and determines which…
Halotypes
combo of alleles on a chromosome... more likely to inherit A1B1…
23 terms
Chapter 11- Biotech | Part 2: Gene Cloning
PLASMIDS AS CLONING VECTORS
Plasmids
Vector Transfer
pUC19
.......
natural vectors with useful properties.... -small size, easy to…
through chemical transformation or electroporation
common cloning vector. ... A modified ColE1 plasmid.... -contains…
PLASMIDS AS CLONING VECTORS
.......
Plasmids
natural vectors with useful properties.... -small size, easy to…
59 terms
Genetic Analysis (Exam 1)
Gastrulation
Patterns of Gastrulation (5)
Acrosomal Reaction
Fast & Instantaneous Block of Polyspermy
-Hallmark: CELL MOVEMENT** characterizes gastrulation.... -Cell…
-Invagination: in folding sheet of cells (like poked rubber b…
-The release of enzyme from the sperm to digest the egg's ext…
Includes the methods of the fertilization envelope and a memb…
Gastrulation
-Hallmark: CELL MOVEMENT** characterizes gastrulation.... -Cell…
Patterns of Gastrulation (5)
-Invagination: in folding sheet of cells (like poked rubber b…
8 terms
Science Biotech & Cloning
Applications of biotechnology
Gel Electrophoresis
Cloning
Restriction enzymes
Basic biological research (Cloning)... Medical diagnostics *... Med…
Restriction enzymes cleave DNA into smaller segments of vario…
Begin with egg with its nucleus removed... Select DNA from the c…
Cut DNA at restriction site (palindromic) ... Bacterial defense…
Applications of biotechnology
Basic biological research (Cloning)... Medical diagnostics *... Med…
Gel Electrophoresis
Restriction enzymes cleave DNA into smaller segments of vario…
17 terms
Techniques of Recombinant DNA II- PCR based cloning & more
PCR figure
PCR-based cloning basics
PCR-based cloning limitations
PCR primers
- differs from traditional cloning in that the DNA fragment o…
- Can't amplify big DNA fragments (2-3 kb) in complex mixture…
- target area of interest... - 18-22 nucleotides in length with…
PCR figure
PCR-based cloning basics
- differs from traditional cloning in that the DNA fragment o…
Ch. 9 Digital analysis of genomes
Two ways to break down DNA in order to…
Classical method
Shotgun Method
Digesting DNA
-Classical Method... -Shotgun Method
:cut DNA at specific sequences (digestion at restriction site…
:shear DNA using psychical methods (Sonication, physical stre…
restriction enzymes cut viral or foreign Dna into fragments,…
Two ways to break down DNA in order to…
-Classical Method... -Shotgun Method
Classical method
:cut DNA at specific sequences (digestion at restriction site…
19 terms
Biology Section 8.1: Genetic Analysis & 8.2: Recombinant Technology
Chromosomal analysis
Karyotype
Amniocentesis
Down syndrome
a technique used to determine chromosomal abnormalities
a method of organizing the chromosomes of a cell in relation…
a procedure in which amniotic fluid containing fetal tissue i…
a human genetic disorder caused by having an extra chromosome…
Chromosomal analysis
a technique used to determine chromosomal abnormalities
Karyotype
a method of organizing the chromosomes of a cell in relation…
10 terms
DNA cloning
biopharmaceuticals (proteins), gene th…
restriction enzymes
DNA ligase
recombinant DNA
DNA cloning can be used to produce___ , attempt____ and ____
___ recognizes and cuts a specific dna sequence
___ joins the phosphate of 5' to hydroxyl of 3' end
___ is DNA assembled from multiple sources
biopharmaceuticals (proteins), gene th…
DNA cloning can be used to produce___ , attempt____ and ____
restriction enzymes
___ recognizes and cuts a specific dna sequence
26 terms
Chapter 14: DNA Cloning
cloning
gene cloning
gene therapy
transgenic organisms
production of genetically identical copies of DNA, cells, or…
where DNA is cloned so that there are many identical copies o…
when cloned genes are used to modify a human
product that is cloned; ... - Contains genes from other species…
cloning
production of genetically identical copies of DNA, cells, or…
gene cloning
where DNA is cloned so that there are many identical copies o…
13 terms
DNA Cloning & Biotechnology Products
Genome
Genetic engineering
Cloning
Gene cloning
Full set of genetic information of a species or virus
Alteration of genomes for medical or industrial purposes
-may be whole organism or just a simple gene... -is the process…
-Many identical copies of one gene... -used to produce genes pro…
Genome
Full set of genetic information of a species or virus
Genetic engineering
Alteration of genomes for medical or industrial purposes
54 terms
Genetics - Genome Analysis
Silent mutation
Missense mutation
Nonsense mutation
Frameshift mutation
last nucleotide is changes and the amino acid remains the same
Changing of a nucleotide codes for a different amino acid
Changing of a nucleotide codes for a stop codon
Indels (in numbers not divisible by 3) in nucleotides that ch…
Silent mutation
last nucleotide is changes and the amino acid remains the same
Missense mutation
Changing of a nucleotide codes for a different amino acid
65 terms
DNA Analysis and Technology
Restriction endonucleases
Sticky ends
EcoRI
DNA Cloning
Recognize specific palindromic DNA seqs and cut in a specific…
The two overhanging sequences are antiparallel and complement…
GAATTC-- cut into G AATTC. Function as immune system for E. c…
Cloning vector is used to create a space for a foreign target…
Restriction endonucleases
Recognize specific palindromic DNA seqs and cut in a specific…
Sticky ends
The two overhanging sequences are antiparallel and complement…
15 terms
Science Cloning
selective breeding
hybrids
another name for cloning
what happens a cloning
The process by which humans breed other animals or plants for…
The cross of animals results in what is called a crossbreed
mitosis
body cell nucleus is entering into egg cell
selective breeding
The process by which humans breed other animals or plants for…
hybrids
The cross of animals results in what is called a crossbreed
62 terms
Biotechnology, Human Genome/Cloning, DNA Tools and Biotechnology, Genetic Engineering, biotechnology biology
Dna Fingerprinting
Human Genome Project
Recombinant DNA
Genetic Engineering
the analysis of dna from samples of body tissues or fluids in…
scientific research to figure out and mapall of human genes a…
dna that has been formed artifically by combining constituent…
the deliberate modification of the characteristics of an orga…
Dna Fingerprinting
the analysis of dna from samples of body tissues or fluids in…
Human Genome Project
scientific research to figure out and mapall of human genes a…
31 terms
COMD 5070 - Module 3 - Acoustic Analysis
Get Good Data
Sound and Movement
Benefits of Acoustic Analysis
Limitations of Acoustic Analysis
1. quiet recording environment... 2. quality microphone... 3. good…
1. all sounds originate w/ movement (string on instrument, os…
1. noninvasive... 2. can draw inferences from changes in acousti…
1. acoustic patterns reflect vocal tract movements w/ some am…
Get Good Data
1. quiet recording environment... 2. quality microphone... 3. good…
Sound and Movement
1. all sounds originate w/ movement (string on instrument, os…
43 terms
CH 17 Recombinant DNA and Gene Cloning
Recombinant DNA
Glo Fish and Glo Pigs
Steps in Cloning a Gene
Each bacterial colony is a clone, carr…
Joining of DNA from different organisms in the test tube... -is…
engineered to express the gene for green fluorescent protein…
1. Isolate DNA from the organism ... 2. Cut DNA with restriction…
the whole thing plated on the medium is a colony, and each ar…
Recombinant DNA
Joining of DNA from different organisms in the test tube... -is…
Glo Fish and Glo Pigs
engineered to express the gene for green fluorescent protein…
14 terms
Pearson Texas Biology: Chapter 15 Genetic Engineering N. Walker
selective breeding
hybridization
inbreeding
biotechnology
method of breeding that allows only those organisms with desi…
breeding technique that involves crossing dissimilar individu…
continued breeding of individuals with similar, desirable cha…
process of manipulating organisms, cells, or molecules, to pr…
selective breeding
method of breeding that allows only those organisms with desi…
hybridization
breeding technique that involves crossing dissimilar individu…
52 terms
Genetics Chapter 19: Molecular Genetic Analysis
Gel Electrophoresis
Size Standards
Recombinant DNA
Biotechnology
-Used to separate nucleic acids based in size ... -Nucleic acids…
Markers of known molecular weight used to create a "ladder" a…
A DNA molecule made in vitro with segments from different sou…
The industry that uses molecular techniques to develop new pr…
Gel Electrophoresis
-Used to separate nucleic acids based in size ... -Nucleic acids…
Size Standards
Markers of known molecular weight used to create a "ladder" a…
31 terms
HR Block 2: Analysis of Genetic Variation
structure of metaphase chromosomes
Banding of metaphase chromosomes
G-banding (Giemsa stain)
Q-banding
reproducible staining pattern of bands numbered from centrome…
-A-T rich... -hterochromatic regions of chromosomes ... -stain dark…
-fluorescent stain... -produces essentially the same banding pat…
structure of metaphase chromosomes
Banding of metaphase chromosomes
reproducible staining pattern of bands numbered from centrome…
13 terms
ck-12 9.2: Gene Cloning - Advanced
What is a clone?
What is gene cloning or molecular clon…
What is engineered through the combina…
What is the process of genetic alterat…
a genetically exact copy
the process of isolating a DNA sequence of interest for the p…
Recombinant DNA, an form of artificial DNA
transformation
What is a clone?
a genetically exact copy
What is gene cloning or molecular clon…
the process of isolating a DNA sequence of interest for the p…
19 terms
GENETICS PART 2
selective breeding
hybridization
inbreeding
mutations
method that allows only those individual organisms with desir…
Crossing different individuals to bring together the best of…
Continued mating of individuals with similar characteristics…
Changes in DNA structure
selective breeding
method that allows only those individual organisms with desir…
hybridization
Crossing different individuals to bring together the best of…
24 terms
Mutations and Genetic Engineering
Mutation
Point Mutation
Frameshift Mutation
Mutagen
A change in an organism's DNA
Incorrect nucleotide is put into a DNA molecule during replic…
The addition or removal of a nucleotide in the DNA sequence
Environments that change DNA
Mutation
A change in an organism's DNA
Point Mutation
Incorrect nucleotide is put into a DNA molecule during replic…
21 terms
Final - Cloning Lec 1-3
**Define the 3 types of restriction en…
** Explain why Type II restriction enz…
** Describe what the arrows positioned…
** Explain how 5' overhangs and 3' ove…
• Type I & III - contain endonuclease & methylase; bind to re…
• does not require ATP ... • recognizes & cleaves at specific re…
• tells you cleavage site & the type of ends you will generat…
• 5' - can be filled in w/ DNA Polymerase & dNTP... • 3' - needs…
**Define the 3 types of restriction en…
• Type I & III - contain endonuclease & methylase; bind to re…
** Explain why Type II restriction enz…
• does not require ATP ... • recognizes & cleaves at specific re…
30 terms
Cloning and GMOs
Selective breeding
hybridization
Inbreeding
Biotechnology
Allowing only those animals with wanted characteristics to pr…
Crossing dissimilar individuals to bring together the best of…
Continue breathing of the individuals similar characteristics
The application of the technological process innovation or me…
Selective breeding
Allowing only those animals with wanted characteristics to pr…
hybridization
Crossing dissimilar individuals to bring together the best of…
27 terms
1C. Transmission of heritable information from generation to generation and the processes that increase genetic diversity
Codon substitution in Sickle Cell Anemia
Which has the potential to be most des…
Nonsense mutations
Missense mutations
Glu to Val
Mistake at the DNA level
results in stop codons
change one amino acid to another
Codon substitution in Sickle Cell Anemia
Glu to Val
Which has the potential to be most des…
Mistake at the DNA level
33 terms
Micro Chapter 18
Isolation
Enrichment cultures
Inoculum
Enrichment cultures
The separation of individual organisms from the mixed community
Select for desired organisms through manipulation of medium a…
The sample from which microorganisms will be isolated
-Can prove the presence of an organism in a habitat... -Cannot p…
Isolation
The separation of individual organisms from the mixed community
Enrichment cultures
Select for desired organisms through manipulation of medium a…
39 terms
Cellular Differentiation and Genetic Analysis
Cellular Differentiation
true
proteins
true
process of converting a stem cell into a more specialized cell
All multicellular organisms have stem cells.... True or False
produced within the cell and control the differentiation proc…
Biochemical agents can be used to initiate cellular different…
Cellular Differentiation
process of converting a stem cell into a more specialized cell
true
All multicellular organisms have stem cells.... True or False
56 terms
Enzymes and Cloning
Enzymes in nature
First report of enzymatic breakdown of…
Nuclease function
The two mechanisms used to break phosp…
Polymerase... Ligase... Nuclease
1903
Break phosphodiester bonds via two mechanisms
Hydrolysis... Lyase
Enzymes in nature
Polymerase... Ligase... Nuclease
First report of enzymatic breakdown of…
1903
Unit 4: DNA Technology (1 of 2)
selective breeding
transgenic
genetically modified organism
hybridization
method in which humans choose individual organisms with desir…
An organism that contains DNA from another species.
GMO
Crossing different individuals to make a genetic cross of bot…
selective breeding
method in which humans choose individual organisms with desir…
transgenic
An organism that contains DNA from another species.
22 terms
VIA Biology Ch. 13 and 14: Genetic Engineering & The Human Genome (Mrs. Robinson)
karyotype
sex chromosome
autosome
pedigree
The number and visual appearance of the chromosomes in the ce…
One of the 23 pairs of chromosomes in the human, contains gen…
A chromosome that is not directly involved in determining sex…
A chart or "family tree" that tracks which members of a famil…
karyotype
The number and visual appearance of the chromosomes in the ce…
sex chromosome
One of the 23 pairs of chromosomes in the human, contains gen…
23 terms
Science Vocab: Biotechnology
Plasmid
Biotechnology
Gene cloning
Gene cloning steps
Deliver: a ring of DNA that carries accessory genes separate…
the use of living organisms in solving a problem or for human…
making a copy of a gene... leads to the production of multiple,…
1. isolate plasmid... 2. identify gene of interest... 3. cut out ge…
Plasmid
Deliver: a ring of DNA that carries accessory genes separate…
Biotechnology
the use of living organisms in solving a problem or for human…
30 terms
Genetic Engineering
selective breeding
hybridization
inbreeding
mutations
method that allows only those individual organisms with desir…
Crossing different individuals to make a genetic cross of bot…
Continued mating of individuals with similar characteristics…
Changes in DNA structure
selective breeding
method that allows only those individual organisms with desir…
hybridization
Crossing different individuals to make a genetic cross of bot…
33 terms
DNA Tech
biotechnology
gel electrophoresis
polymerase
denaturation
...
...
...
...
biotechnology
...
gel electrophoresis
...
27 terms
GENETIC ENGINEERING VOCABULARY
selective breeding
hybridization
inbreeding
mutations
method that allows only those individual organisms with desir…
Crossing different individuals to bring together the best of…
Continued mating of individuals with similar characteristics…
Changes in DNA structure
selective breeding
method that allows only those individual organisms with desir…
hybridization
Crossing different individuals to bring together the best of…
15 terms
Science
nanoscience
vehicle
digital
web
The study of molecules and nanostructures whose size ranges f…
something in which people or goods are carried from place to…
A worldwide gap giving advantage to those with access to tech…
difference thresholds increase in proportion to the size of t…
nanoscience
The study of molecules and nanostructures whose size ranges f…
vehicle
something in which people or goods are carried from place to…
30 terms
Genetics: The Key of Life
Allele
Dominant Allele
Recessive Allele
Homozygous
The different forms of a gene
An allele whose trait always shows up in the organism when th…
An allele that is masked when a dominant allele is present
Having two identical alleles for a trait
Allele
The different forms of a gene
Dominant Allele
An allele whose trait always shows up in the organism when th…
47 terms
INTRO to BIOLOGY PRE-ASSESS, ECOLOGY
Cells
Unicellular
Multicellular
Organization
Basic unit of life
Organism made of only one cell
Organism composed of many cells
arranged in an orderly way; cells, tissues, organs, systems,…
Cells
Basic unit of life
Unicellular
Organism made of only one cell
37 terms
INTRO to BIOLOGY PRE-ASSESS
Cells
Unicellular
Multicellular
Organization
Basic unit of life
Organism made of only one cell
Organism composed of many cells
arranged in an orderly way; cells, tissues, organs, systems,…
Cells
Basic unit of life
Unicellular
Organism made of only one cell
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