Study sets matching "cloning analysis"

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Study sets matching "cloning analysis"

Analysis of the Genome (Cloning)
A group of cells that are all descenda…
What are the three approaches to cloni…
A long-lived collection of cellular cl…
__________include an origin for DNA re…
Cellular Clone... DNA Clone
1) Genomic library-Collection of cloned genomic DNA fragments…
Genomic Library
Vectors
A group of cells that are all descenda…
Cellular Clone... DNA Clone
What are the three approaches to cloni…
1) Genomic library-Collection of cloned genomic DNA fragments…
36 terms
Cloning and Molecular Analysis of Genes
DNA can be undergo replication. DNA tr…
DNA polymerase, DNA ligase, Reverse tr…
Restriction enzyme
Methylase
Central dogma of Molecular Genetics
Enzymes for DNA analysis and molecular cloning
cleave DNA in a sequence specific manner.
DNA methylation involved the addition of a methyl group to DNA.
DNA can be undergo replication. DNA tr…
Central dogma of Molecular Genetics
DNA polymerase, DNA ligase, Reverse tr…
Enzymes for DNA analysis and molecular cloning
DNA cloning/analysis
A gene
Coding regions
Gene components
Simple eukaryotic transcription unit
Entire DNA sequence required for synthesis of a functional pr…
Exon
Exons, control regions, introns
Single monocistronic mRNA
A gene
Entire DNA sequence required for synthesis of a functional pr…
Coding regions
Exon
16 terms
Clone Isolation/Analysis & Cancer
DNA Probes
Southern Blot Analysis
Polymerase Chain Reaction
Sanger Sequencing
Short single-stranded stretches of DNA of known composition.…
Used to show the location of a gene within a larger fragment…
PCR - A fast inexpensive method of replicating a DNA sequence…
Reveals the order of base pairs in an isolated DNA molecule.…
DNA Probes
Short single-stranded stretches of DNA of known composition.…
Southern Blot Analysis
Used to show the location of a gene within a larger fragment…
Chapter 7: Cloning Genes for Analysis
Chimera
Cloning vecotr
Vector
ColE1 plasmid
Hybrid molecule of DNA that has DNA form more than one source…
Any molecule of DNA that can replicate itself inside a cell a…
In molecular biology, a vector is a molecule of DNA which can…
Small multicopy plasmid of Escherichia coli that forms the ba…
Chimera
Hybrid molecule of DNA that has DNA form more than one source…
Cloning vecotr
Any molecule of DNA that can replicate itself inside a cell a…
9 terms
PCR, Gene Cloning, DNA/Protein Purification and Analysis
What do you use as a cloning vector?
plasmids
What does a plasmid contain?
origin of replication
plasmids
self-replicating, circular DNA molecules found in bacteria
A typical plasmid includes an origin of replication site, mul…
where DNA replication begins (effective vs. ineffective sites
What do you use as a cloning vector?
plasmids
plasmids
self-replicating, circular DNA molecules found in bacteria
12 terms
Lecture 7 - Cloning actin gene in chicken - northern blot analysis
Protocol when cloning eukaryotic gene?
What is the next step following obtain…
Assessing tissue specific gene express…
Assessing developmental gene expression?
Perform the standard cloning steps with parallel gel enrichme…
Performing restriction enzyme mapping of positive clones and…
Isolate RNA from various organs / tissue types -> perform nor…
Take various time points of embryo & adult development RNA ti…
Protocol when cloning eukaryotic gene?
Perform the standard cloning steps with parallel gel enrichme…
What is the next step following obtain…
Performing restriction enzyme mapping of positive clones and…
Genetics and Biotechnology: Cloning Section 5 and Human DNA Analysis Section 6
Clone
DNA Fingerprinting
Gene Therapy
D. all of the above
member of a population of genetically identical organisms pro…
analysis of sections of DNA that have little or no known func…
an absent or faulty gene is replaced by a normal, working gene
Genetic engineering involves... A. reading a DNA sequence... B. edi…
Clone
member of a population of genetically identical organisms pro…
DNA Fingerprinting
analysis of sections of DNA that have little or no known func…
8 terms
BIO 326 - Genetics: Gene Cloning; DNA Sequencing; Genomics and Sequence Analysis
Bioinformatics
Contigs
Dideoxynucleotide triphosphate
DNA sequencing
application of computer-bases information technology to study…
overlapping, continguous DNA sequences
chain terminating nucleotide
create recombinant DNA
Bioinformatics
application of computer-bases information technology to study…
Contigs
overlapping, continguous DNA sequences
7 terms
Lecture 6: (16S PCR, Phylogeny analysis, Gene clone library, massive parallel DNA sequencing, etc.)
16S RNA
Phylogeny analysis
16S rRNA gene clone library
Massively parallel DNA sequencing
- A "mosaic" of regions that are highly conserved among all b…
- Inferred evolutionary relationships between bacterial seque…
Advantages:... - 16S genes are large enough (~1.5 kb) to contain…
- aka "NextGen" or "high-throughput" sequencing... - Can be quan…
16S RNA
- A "mosaic" of regions that are highly conserved among all b…
Phylogeny analysis
- Inferred evolutionary relationships between bacterial seque…
Cloning
cloning
reproductive cloning
therapeutic cloning
recombinant DNA technology
making copies of genes, animals, cells
whole animal cloning
cloning stem cells
A series of procedures that are used to join together (recomb…
cloning
making copies of genes, animals, cells
reproductive cloning
whole animal cloning
Science Biotech & Cloning
Applications of biotechnology
Gel Electrophoresis
Cloning
Restriction enzymes
Basic biological research (Cloning)... Medical diagnostics *... Med…
Restriction enzymes cleave DNA into smaller segments of vario…
Begin with egg with its nucleus removed... Select DNA from the c…
Cut DNA at restriction site (palindromic) ... Bacterial defense…
Applications of biotechnology
Basic biological research (Cloning)... Medical diagnostics *... Med…
Gel Electrophoresis
Restriction enzymes cleave DNA into smaller segments of vario…
21 terms
Gene Mapping and Cloning
Linkage analysis
Halotypes
Syntenic
Mapping by recombination
locates genes on regions of chromosomes and determines which…
combo of alleles on a chromosome... more likely to inherit A1B1…
genes on same chromosome
chromosomes reorganize at meiosis... multiple crossovers can occ…
Linkage analysis
locates genes on regions of chromosomes and determines which…
Halotypes
combo of alleles on a chromosome... more likely to inherit A1B1…
59 terms
Genetic Analysis (Exam 1)
Gastrulation
Patterns of Gastrulation (5)
Acrosomal Reaction
Fast & Instantaneous Block of Polyspermy
-Hallmark: CELL MOVEMENT** characterizes gastrulation.... -Cell…
-Invagination: in folding sheet of cells (like poked rubber b…
-The release of enzyme from the sperm to digest the egg's ext…
Includes the methods of the fertilization envelope and a memb…
Gastrulation
-Hallmark: CELL MOVEMENT** characterizes gastrulation.... -Cell…
Patterns of Gastrulation (5)
-Invagination: in folding sheet of cells (like poked rubber b…
Chapter 11- Biotech | Part 2: Gene Cloning
PLASMIDS AS CLONING VECTORS
Plasmids
Vector Transfer
pUC19
.......
natural vectors with useful properties.... -small size, easy to…
through chemical transformation or electroporation
common cloning vector. ... A modified ColE1 plasmid.... -contains…
PLASMIDS AS CLONING VECTORS
.......
Plasmids
natural vectors with useful properties.... -small size, easy to…
DNA cloning
biopharmaceuticals (proteins), gene th…
restriction enzymes
DNA ligase
recombinant DNA
DNA cloning can be used to produce___ , attempt____ and ____
___ recognizes and cuts a specific dna sequence
___ joins the phosphate of 5' to hydroxyl of 3' end
___ is DNA assembled from multiple sources
biopharmaceuticals (proteins), gene th…
DNA cloning can be used to produce___ , attempt____ and ____
restriction enzymes
___ recognizes and cuts a specific dna sequence
7 terms
Gene Cloning
Plasmid
Restriction Enzyme
Sticky Ends
Ligase
Circular piece of DNA found in bacteria
Enzyme used to cut DNA at specific sites
The ends of DNA after being cut by restriction enzymes
Used to glue the human DNA into the plasmid
Plasmid
Circular piece of DNA found in bacteria
Restriction Enzyme
Enzyme used to cut DNA at specific sites
Cloning
why clone genes
two ways to make gene copies
things you need to clone in bacteria
restriction enzymes
it's easier to manipulate cloned genes: to study genes/gene p…
1. insert a gene into a vector and express it in bacteria... 2.…
plasmid vector, gene of interest, DNA ligase, and restriction…
cut DNA at a recognition sequence
why clone genes
it's easier to manipulate cloned genes: to study genes/gene p…
two ways to make gene copies
1. insert a gene into a vector and express it in bacteria... 2.…
Chapter 14: DNA Cloning
cloning
gene cloning
gene therapy
transgenic organisms
production of genetically identical copies of DNA, cells, or…
where DNA is cloned so that there are many identical copies o…
when cloned genes are used to modify a human
product that is cloned; ... - Contains genes from other species…
cloning
production of genetically identical copies of DNA, cells, or…
gene cloning
where DNA is cloned so that there are many identical copies o…
Techniques of Recombinant DNA II- PCR based cloning & more
PCR figure
PCR-based cloning basics
PCR-based cloning limitations
PCR primers
- differs from traditional cloning in that the DNA fragment o…
- Can't amplify big DNA fragments (2-3 kb) in complex mixture…
- target area of interest... - 18-22 nucleotides in length with…
PCR figure
PCR-based cloning basics
- differs from traditional cloning in that the DNA fragment o…
52 terms
Genetics Chapter 19: Molecular Genetic Analysis
Gel Electrophoresis
Size Standards
Recombinant DNA
Biotechnology
-Used to separate nucleic acids based in size ... -Nucleic acids…
Markers of known molecular weight used to create a "ladder" a…
A DNA molecule made in vitro with segments from different sou…
The industry that uses molecular techniques to develop new pr…
Gel Electrophoresis
-Used to separate nucleic acids based in size ... -Nucleic acids…
Size Standards
Markers of known molecular weight used to create a "ladder" a…
Science Cloning
selective breeding
hybrids
another name for cloning
what happens a cloning
The process by which humans breed other animals or plants for…
The cross of animals results in what is called a crossbreed
mitosis
body cell nucleus is entering into egg cell
selective breeding
The process by which humans breed other animals or plants for…
hybrids
The cross of animals results in what is called a crossbreed
30 terms
Cloning and GMOs
Selective breeding
hybridization
Inbreeding
Biotechnology
Allowing only those animals with wanted characteristics to pr…
Crossing dissimilar individuals to bring together the best of…
Continue breathing of the individuals similar characteristics
The application of the technological process innovation or me…
Selective breeding
Allowing only those animals with wanted characteristics to pr…
hybridization
Crossing dissimilar individuals to bring together the best of…
21 terms
Final - Cloning Lec 1-3
**Define the 3 types of restriction en…
** Explain why Type II restriction enz…
** Describe what the arrows positioned…
** Explain how 5' overhangs and 3' ove…
• Type I & III - contain endonuclease & methylase; bind to re…
• does not require ATP ... • recognizes & cleaves at specific re…
• tells you cleavage site & the type of ends you will generat…
• 5' - can be filled in w/ DNA Polymerase & dNTP... • 3' - needs…
**Define the 3 types of restriction en…
• Type I & III - contain endonuclease & methylase; bind to re…
** Explain why Type II restriction enz…
• does not require ATP ... • recognizes & cleaves at specific re…
CH 17 Recombinant DNA and Gene Cloning
Recombinant DNA
Glo Fish and Glo Pigs
Steps in Cloning a Gene
Each bacterial colony is a clone, carr…
Joining of DNA from different organisms in the test tube... -is…
engineered to express the gene for green fluorescent protein…
1. Isolate DNA from the organism ... 2. Cut DNA with restriction…
the whole thing plated on the medium is a colony, and each ar…
Recombinant DNA
Joining of DNA from different organisms in the test tube... -is…
Glo Fish and Glo Pigs
engineered to express the gene for green fluorescent protein…
19 terms
Biology Section 8.1: Genetic Analysis & 8.2: Recombinant Technology
Chromosomal analysis
Karyotype
Amniocentesis
Down syndrome
a technique used to determine chromosomal abnormalities
a method of organizing the chromosomes of a cell in relation…
a procedure in which amniotic fluid containing fetal tissue i…
a human genetic disorder caused by having an extra chromosome…
Chromosomal analysis
a technique used to determine chromosomal abnormalities
Karyotype
a method of organizing the chromosomes of a cell in relation…
DNA Cloning; lecture 21
Recombinant DNA (Rekombinante DNS)
Genetic engineering (Gentechnologie)
Genes are minute, and there are thousa…
Restriction enzyme (Restriktionsenzym)
Set of molecular tools used for locating, isolating, altering…
application of recombinant DNA technology to solve practical…
selective amplification of your favorite gene (often abbrevia…
Restriction enzymes (restriction endonucleases) are DNAses ex…
Recombinant DNA (Rekombinante DNS)
Set of molecular tools used for locating, isolating, altering…
Genetic engineering (Gentechnologie)
application of recombinant DNA technology to solve practical…
65 terms
DNA Analysis and Technology
Restriction endonucleases
Sticky ends
EcoRI
DNA Cloning
Recognize specific palindromic DNA seqs and cut in a specific…
The two overhanging sequences are antiparallel and complement…
GAATTC-- cut into G AATTC. Function as immune system for E. c…
Cloning vector is used to create a space for a foreign target…
Restriction endonucleases
Recognize specific palindromic DNA seqs and cut in a specific…
Sticky ends
The two overhanging sequences are antiparallel and complement…
33 terms
Cloning and DNA Technology
clone
regeneration
nuclear transplantation
reproductive cloning
an organism produced through asexual reproduction from a sing…
the regrowth of lost body parts
method of animal cloning; involves replacing the nucleus of a…
cloning that results in the birth of a new living individual
clone
an organism produced through asexual reproduction from a sing…
regeneration
the regrowth of lost body parts
29 terms
Gene Technology and Cloning
The 4 basic steps of genetic engineering
restriction enzymes
vector
plasmids
1. Cutting DNA: the DNA from the organism containing the gene…
bacterial enzymes that recognize and bind to specific short s…
an agent that is used to carry the gene of interest into anot…
circular DNA molecules that can replicate independently of th…
The 4 basic steps of genetic engineering
1. Cutting DNA: the DNA from the organism containing the gene…
restriction enzymes
bacterial enzymes that recognize and bind to specific short s…
31 terms
COMD 5070 - Module 3 - Acoustic Analysis
Get Good Data
Sound and Movement
Benefits of Acoustic Analysis
Limitations of Acoustic Analysis
1. quiet recording environment... 2. quality microphone... 3. good…
1. all sounds originate w/ movement (string on instrument, os…
1. noninvasive... 2. can draw inferences from changes in acousti…
1. acoustic patterns reflect vocal tract movements w/ some am…
Get Good Data
1. quiet recording environment... 2. quality microphone... 3. good…
Sound and Movement
1. all sounds originate w/ movement (string on instrument, os…
62 terms
Biotechnology, Human Genome/Cloning, DNA Tools and Biotechnology, Genetic Engineering, biotechnology biology
Dna Fingerprinting
Human Genome Project
Recombinant DNA
Genetic Engineering
the analysis of dna from samples of body tissues or fluids in…
scientific research to figure out and mapall of human genes a…
dna that has been formed artifically by combining constituent…
the deliberate modification of the characteristics of an orga…
Dna Fingerprinting
the analysis of dna from samples of body tissues or fluids in…
Human Genome Project
scientific research to figure out and mapall of human genes a…
Molecular Cloning
restriction endonucleases
Where were restriction enzymes discove…
Why do bacteria use restriction enzymes?
Restriction site
-cleave DNA at specific sequences... -allow long pieces of DNA t…
bacteria
to protect themselves from bacteria
where the restriction enzyme binds to and cuts a specific DNA…
restriction endonucleases
-cleave DNA at specific sequences... -allow long pieces of DNA t…
Where were restriction enzymes discove…
bacteria
Ch. 9 Digital analysis of genomes
Two ways to break down DNA in order to…
Classical method
Shotgun Method
Digesting DNA
-Classical Method... -Shotgun Method
:cut DNA at specific sequences (digestion at restriction site…
:shear DNA using psychical methods (Sonication, physical stre…
restriction enzymes cut viral or foreign Dna into fragments,…
Two ways to break down DNA in order to…
-Classical Method... -Shotgun Method
Classical method
:cut DNA at specific sequences (digestion at restriction site…
Biology Cloning 11-3-16
Stem cells
Human genome project
Cloning
Biotechnology
An undifferentiated cell that is capable of dividing and givi…
an international effort to sequence and map all the DNA on th…
a method of asexual reproduction that produces identical orga…
the study and manipulation of living things or their componen…
Stem cells
An undifferentiated cell that is capable of dividing and givi…
Human genome project
an international effort to sequence and map all the DNA on th…
13 terms
Final - Genomic vs cDNA cloning module L1-3
**Compare genomic cloning, cDNA clonin…
**Outline the general process involved…
**Explain why recombinant bacteriophag…
**Outline the basic screening process…
...
...
...
...
**Compare genomic cloning, cDNA clonin…
...
**Outline the general process involved…
...
52 terms
Genetics Chapter 19: Molecular Genetic Analysis
Gel Electrophoresis
Size Standards
Recombinant DNA
Biotechnology
-Used to separate nucleic acids based in size ... -Nucleic acids…
Markers of known molecular weight used to create a "ladder" a…
A DNA molecule made in vitro with segments from different sou…
The industry that uses molecular techniques to develop new pr…
Gel Electrophoresis
-Used to separate nucleic acids based in size ... -Nucleic acids…
Size Standards
Markers of known molecular weight used to create a "ladder" a…
31 terms
HR Block 2: Analysis of Genetic Variation
structure of metaphase chromosomes
Banding of metaphase chromosomes
G-banding (Giemsa stain)
Q-banding
reproducible staining pattern of bands numbered from centrome…
-A-T rich... -hterochromatic regions of chromosomes ... -stain dark…
-fluorescent stain... -produces essentially the same banding pat…
structure of metaphase chromosomes
Banding of metaphase chromosomes
reproducible staining pattern of bands numbered from centrome…
13 terms
ck-12 9.2: Gene Cloning - Advanced
What is a clone?
What is gene cloning or molecular clon…
What is engineered through the combina…
What is the process of genetic alterat…
a genetically exact copy
the process of isolating a DNA sequence of interest for the p…
Recombinant DNA, an form of artificial DNA
transformation
What is a clone?
a genetically exact copy
What is gene cloning or molecular clon…
the process of isolating a DNA sequence of interest for the p…
Chapter 20: Biotechnology *see notes for cloning!*
Restriction Enzyme
Restriction Site
Sticky End
Restriction Fragment
protects bacteria from phages by cutting up foreign DNA
short nucleotide sequences that are cut by enzymes; usually s…
sugar phosphate backbone cut in staggered way
cut up piece of DNA
Restriction Enzyme
protects bacteria from phages by cutting up foreign DNA
Restriction Site
short nucleotide sequences that are cut by enzymes; usually s…
Molecular Genetic Methods: Cloning, PCR and Sequencing
recombinant DNA
DNA cloning using plasmids
restriction enzyme
palindromic
any DNA molecule composed of sequences derived from different…
recombinant DNA technique in which specific cDNAs or fragment…
endonuclease that cleaves phosphodiester bond on both strands…
sequences of DNA are the same on each strand when read 5' to 3'
recombinant DNA
any DNA molecule composed of sequences derived from different…
DNA cloning using plasmids
recombinant DNA technique in which specific cDNAs or fragment…
Honors Biology: Cloning & Chapter 12 Vocab Quiz
clone
regeneration
nuclear transportation
reproductive cloning
an organism produced through asexual reproduction from a sing…
regrowth of lost body parts; indication we don't need differe…
used in animal cloning; involves replacing the nucleus of a g…
uses nuclear transportation and results in the birth of a new…
clone
an organism produced through asexual reproduction from a sing…
regeneration
regrowth of lost body parts; indication we don't need differe…
19 terms
Quiz #3: Cloning and Separation
Alkaline phosphatase
Prevents religation
Phage vectors
Charon 4
removes terminal phosphates in a cleaved plasmid DNA strand t…
the use of alkaline phosphatase or two endonucleases (as oppo…
linear, double-stranded DNA derivatives of viral genomes that…
a lambda phage replacement vector that allows DNA inserts to…
Alkaline phosphatase
removes terminal phosphates in a cleaved plasmid DNA strand t…
Prevents religation
the use of alkaline phosphatase or two endonucleases (as oppo…
72 terms
Intro to Genetic Analysis Chapter 1 Key words
Active site
Allele
Bioformatics
Codon
The part of a protein that must be maintained in a specific s…
One of the different forms of a gene that can exist at a sing…
Computational information systems and analytical methods appl…
A section of DNA (three nucleotide pairs in length) that enco…
Active site
The part of a protein that must be maintained in a specific s…
Allele
One of the different forms of a gene that can exist at a sing…
39 terms
Cellular Differentiation and Genetic Analysis
Cellular Differentiation
true
proteins
true
process of converting a stem cell into a more specialized cell
All multicellular organisms have stem cells.... True or False
produced within the cell and control the differentiation proc…
Biochemical agents can be used to initiate cellular different…
Cellular Differentiation
process of converting a stem cell into a more specialized cell
true
All multicellular organisms have stem cells.... True or False
Biology Chapter 15
selective breeding
hybridization
inbreeding
biotechnology
The process of selecting a few organisms with desired traits…
(genetics) the act of mixing different species or varieties o…
Continued breeding of individuals with similar characteristic…
A form of technology that uses living organisms, usually gene…
selective breeding
The process of selecting a few organisms with desired traits…
hybridization
(genetics) the act of mixing different species or varieties o…
Digital Analysis of Genomes
restriction enzymes
blunt ends
sticky ends
4^n
fragment the DNA at certain sites by cutting the sugar-phosph…
cuts made straight through the double strand
cuts that are equally displaced and leave a 3' or 5' overhang
average bp between recognition sites, n = length of site and…
restriction enzymes
fragment the DNA at certain sites by cutting the sugar-phosph…
blunt ends
cuts made straight through the double strand
38 terms
Analysis of Genes
Allele
Homie Gregor Mendel
Archibald Garrod
Independent Assortment
Variant form of a gene
-pioneer in the experimental analysis of inheritance... -determi…
-proposed that a specific disease could be the result of a he…
-Originated by Mendel... -when two or more characteristics are i…
Allele
Variant form of a gene
Homie Gregor Mendel
-pioneer in the experimental analysis of inheritance... -determi…
5 terms
8L21 Biotechnology
biotechnology
genetic modification
genome
cloning
A form of technology that uses living organisms, usually gene…
Branch of biotechnology that alters the genes in biological o…
All the genetic information in an organism; all of an organis…
Making a genetically identical copy of DNA or of an organism.
biotechnology
A form of technology that uses living organisms, usually gene…
genetic modification
Branch of biotechnology that alters the genes in biological o…
17 terms
Chapter 12 Digital Analysis of DNA
The general ideas behind genome sequen…
Restriction enzyme
Recognition sites for restriction enzy…
Restriction enzymes produce restrictio…
fragmenting the genome, cloning DNA fragments, sequencing DNA…
recognizes a specific sequence of bases anywhere within the g…
often palindromic- base sequences of each strand are identica…
Blunt Ends- cuts are straight through both DNA strands at the…
The general ideas behind genome sequen…
fragmenting the genome, cloning DNA fragments, sequencing DNA…
Restriction enzyme
recognizes a specific sequence of bases anywhere within the g…
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