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Study sets matching "cloning"

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Study sets matching "cloning"

17 terms
Biology Cloning
Biotechnology
Genetic Engineering
Restriction Enzyme
Polymerase Chain Reaction (PCR)
The modifictation of organisms, cells, and their molecules fo…
The manipulation of an organism's genetic material by adding,…
Enzyme used to cut DNA into small pieces
Lab technique that allows tiny pieces of DNA to be duplicated…
Biotechnology
The modifictation of organisms, cells, and their molecules fo…
Genetic Engineering
The manipulation of an organism's genetic material by adding,…
11 terms
Animal cloning
Cloning is making an exact
Clones are described as
Cloning occurs
Identical twins are
copy of another organism
genetically identical organisms
naturally in plants and animals
clones of each other
Cloning is making an exact
copy of another organism
Clones are described as
genetically identical organisms
13 terms
Human Cloning
identical
unpredictability
ethicist
traits
identisk
uforudsigelig
etiker
træk
identical
identisk
unpredictability
uforudsigelig
8 terms
Animal Cloning
Stage 1
Stage 2
Stage 3
Stage 4
A diploid (normal) body cell is taken (e.g. a liver cell)
The nucleus is taken out of it (full number of chromosomes) a…
An unfertilised egg cell is taken, the nucleus (with half the…
The nucleus of the body cell is injected into the cytoplasm o…
Stage 1
A diploid (normal) body cell is taken (e.g. a liver cell)
Stage 2
The nucleus is taken out of it (full number of chromosomes) a…
44 terms
DNA cloning
Cloning
An underground stem or root sends up n…
Members of a bacterial colony on a pet…
Human identical twins are clones because
Is the production of identical copies of DNA through some sex…
parent plant
they all came from division of the same cell
the original triangle embryo separate to become two individuals
Cloning
Is the production of identical copies of DNA through some sex…
An underground stem or root sends up n…
parent plant
23 terms
Cloning speaking
Этический
Научный
Религиозный
Клонирование
Ethical
Scientific
Religious
Cloning
Этический
Ethical
Научный
Scientific
15 terms
Classic Cloning
Classic Approach
Convert mRNA to ssDNA
Convert ssDNA to dsDNA
Restricting DNA and Cloning Vectors
first move is isolate total RNA from tissue, then purify mRNA…
mRNA with Reverse Transcriptase and deoxynucleotides yield si…
ssDNA+ deoxynucleotides + DNA dependent, DNA polymerase yield…
always insert dsDNA into the cloning vector... opening is by dig…
Classic Approach
first move is isolate total RNA from tissue, then purify mRNA…
Convert mRNA to ssDNA
mRNA with Reverse Transcriptase and deoxynucleotides yield si…
16 terms
Human cloning
Hailed
Impending
Proponent
Infertility
Okrzyknięty
Nieuchronnie zbliżający się
Zwolennik
Bezpłodność
Hailed
Okrzyknięty
Impending
Nieuchronnie zbliżający się
37 terms
Therapeutic cloning
Ailment
Breakthrough
Clinical trial
Condition
Maladie
Découverte capitale
Essai clinique
Maladie
Ailment
Maladie
Breakthrough
Découverte capitale
14 terms
contagious - cloning
contagious
mutagen
vector
Carrier
disease that is spread from one organism to another
anything that can change the DNA of an organism
organism that helps spread disease
an organism that is infected with the disease and can transmi…
contagious
disease that is spread from one organism to another
mutagen
anything that can change the DNA of an organism
18 terms
Biology: Cloning
Clones
Asexual reproduction
Embryo
Nuclear transfer
Genetically identical and that all have the same DNA as the o…
Cloning which involves only one parent
A ball of cells made from fertilisation
Process using the DNA in the nucleus of udder cells
Clones
Genetically identical and that all have the same DNA as the o…
Asexual reproduction
Cloning which involves only one parent
23 terms
ERCW Cloning
iconoclastic
octogenarian
deluged
elude
(adj.) attacking or seeking to overthrow popular or tradition…
(adj.) between the ages of 80 and 90 years ol
flooded or overwhelmed
(v.) to escape, either physically or mentally
iconoclastic
(adj.) attacking or seeking to overthrow popular or tradition…
octogenarian
(adj.) between the ages of 80 and 90 years ol
ERWC Cloning
Iconoclastic
Octogenarian
Deluged
Elude
(adj.) attacking or seeking to overthrow popular or tradition…
adj. A person of between eighty and ninety years.
flooded or overwhelmed
(v.) to escape, either physically or mentally
Iconoclastic
(adj.) attacking or seeking to overthrow popular or tradition…
Octogenarian
adj. A person of between eighty and ninety years.
17 terms
Molecular Cloning
cloning
molecular cloning
Recombinant DNA technology
cloning vectors (plasmids)
production of cells or organisms of a single type isolated an…
selective amplification of a particular DNA or a segment of a…
1. cutting DNA at precise locations... 2.selecting a small molec…
used as a delivery agent for transferring DNA into organisms
cloning
production of cells or organisms of a single type isolated an…
molecular cloning
selective amplification of a particular DNA or a segment of a…
11 terms
Biotechnoloy: Cloning
Three types of cloning
Two types of gene cloning
creating an exact copy of a particular…
Two methods of organismal cloning
gene cloning, reproductive or organismal cloning, therapeutic…
PCR, cloning vectors
reproductive cloning
embryo twinning, somatic cell nuclear transfer
Three types of cloning
gene cloning, reproductive or organismal cloning, therapeutic…
Two types of gene cloning
PCR, cloning vectors
13 terms
DNA cloning
To clone researches need to:
gene cloning
Two purposes of gene cloning
Restriction enzymes
-obtain a plasmid and insert foreign DNA ... -Plasmid turns into…
-the production of multiple copies of the same gene
-amplify a gene ... -Produce particular protein products
-restriction endonuclease (cuts in the middle) ... -recognizes a…
To clone researches need to:
-obtain a plasmid and insert foreign DNA ... -Plasmid turns into…
gene cloning
-the production of multiple copies of the same gene
32 terms
DNA cloning
DNA cloning
process of cloning
dont need the excess
removing excess dna/genes
making copies of a DNA strand; need multiple copies of DNA to…
the gene can be pro or euk; the gene will be a small part of…
you remove the excess dna/ gene around the gene of interest b…
restriction enzymes cut dna at a specific sequence and makes…
DNA cloning
making copies of a DNA strand; need multiple copies of DNA to…
process of cloning
the gene can be pro or euk; the gene will be a small part of…
9 terms
GENE CLONING
items required for gene cloning
restriction endonuclease
describe the natural function of restr…
formation of recombinant DNA molecule
1 restriction enzyme... 2 DNA ligase... 3 cloning vector... 4 gene of…
the enzyme that binds to a double stranded DNA molecule at a…
protective mechanism to help bacteria resist attack from bact…
...
items required for gene cloning
1 restriction enzyme... 2 DNA ligase... 3 cloning vector... 4 gene of…
restriction endonuclease
the enzyme that binds to a double stranded DNA molecule at a…
Model lesson - GM-Cloning
Cloning
Nature
Nurture
Theorist
Cloning
Nature
25 terms
Cancer Cloning
carcinogens
origin of cancer
cancer by loss of specialization
squamous cell carcinoma
substances that cause cancer by daming DNA
-activation of stem cells that produce cancer cells... -dediffer…
-specialized cells lose some features as mutations occur when…
cancer that forms in squamous cells
carcinogens
substances that cause cancer by daming DNA
origin of cancer
-activation of stem cells that produce cancer cells... -dediffer…
11 terms
Animal cloning
Cloning is making an exact
Clones are described as
Cloning occurs
Identical twins are
copy of another organism
genetically identical organisms
naturally in plants and animals
clones of each other
Cloning is making an exact
copy of another organism
Clones are described as
genetically identical organisms
10 terms
Lambda Cloning
Advantages of Lambda Cloning
Lambda Phage Lifecycles
Replication process of lambda phage
What determines the amount of DNA in t…
- Generate millions of clones that can be handled easily... - Ca…
1) Lysogenic: lambda DNA integrates into the host chromosome…
1) DNA injected into E. coli in a linear form... 2) cos sites on…
The cos sites
Advantages of Lambda Cloning
- Generate millions of clones that can be handled easily... - Ca…
Lambda Phage Lifecycles
1) Lysogenic: lambda DNA integrates into the host chromosome…
14 terms
CLONING/BIOETHICS
Identical twins are
Natural clones
Chimera
Embryos that are genetically different…
natural clones
they are identical genetically formed when a single embryo br…
an individual whose cells are genetically different
combine into one another in the womb
Identical twins are
natural clones
Natural clones
they are identical genetically formed when a single embryo br…
133 terms
Gene Cloning
plasmid
restriction enzyme
ligation
transformation
circular self-replicating non-chromosomal prokaryotic DNA
prokaryotic endonuclease specific to nucleotide sequence
joining of two pieces of DNA, reforming the phosphodiester li…
taking up of a plasmid by bacteria, thereby changing the gene…
plasmid
circular self-replicating non-chromosomal prokaryotic DNA
restriction enzyme
prokaryotic endonuclease specific to nucleotide sequence
20 terms
GENE CLONING
Bio technology
DNA technology
What is blood sugar
Recombinant DNA
The manipulation of living things to make useful products... Ma…
The manipulation of genetic material... It means modifying or m…
Glucose
The term for combining genes from two different sources to fo…
Bio technology
The manipulation of living things to make useful products... Ma…
DNA technology
The manipulation of genetic material... It means modifying or m…
12 terms
animal cloning
what are totipotent stem cells
what are animal clones
methods of artificially cloning animals
embryo splitting method
stem cells capable of differentiating into any type of cell f…
genetically identical organisms with the same genotype from t…
embryo splitting... nuclear transfer
sperm and egg fertilise to form a zygote that grows into a ba…
what are totipotent stem cells
stem cells capable of differentiating into any type of cell f…
what are animal clones
genetically identical organisms with the same genotype from t…
16 terms
Genetics Cloning
P1 Buffer
P2 Buffer
N3 Buffer
PB Buffer
(1)RNAse to destroy RNA so that we only obtain DNA... To dissolv…
(2)Lysis buffer, (contains Detergent, SDS, and is highly alka…
(3)High salt content to precipitate DNA; neutralizes P2 Buffe…
(4)A was to remove endonucleases (which would chop DNA)
P1 Buffer
(1)RNAse to destroy RNA so that we only obtain DNA... To dissolv…
P2 Buffer
(2)Lysis buffer, (contains Detergent, SDS, and is highly alka…
Gene Cloning
Restriction Endonucleases
Ligases
Phosphotases
Nucleases
Enzymes that cut the DNA and the vector. Ends produced must b…
Enzymes that form 2 covalent bonds between 3'OH ends of one n…
Removes 5' phosphate groups from vectors to prevent self-liga…
Enzyme capable of cleaving the phosphodiester bonds between n…
Restriction Endonucleases
Enzymes that cut the DNA and the vector. Ends produced must b…
Ligases
Enzymes that form 2 covalent bonds between 3'OH ends of one n…
15 terms
Biology cloning
Enzymes
Restriction enzyme
Ligase enzyme
Recombinant dna
Cut up and join dna pieces together
Recognize specific sequences of dna and cut at these points
Join 2 pieces of dna together
2 different bits of Dan stuck together
Enzymes
Cut up and join dna pieces together
Restriction enzyme
Recognize specific sequences of dna and cut at these points
11 terms
Animal Cloning
Main advantage of cloning from adult c…
Dolly the Sheep was produced from
Requires source of recipient eggs
enucleation
Allows evaluation of phenotype... BUT environment interacts wit…
adult mammary cell
Need to be from species of interest... Slaughter ovaries are a…
removal of oocyte nucleus by gentle aspiration
Main advantage of cloning from adult c…
Allows evaluation of phenotype... BUT environment interacts wit…
Dolly the Sheep was produced from
adult mammary cell
12 terms
Fertilisation and cloning
IVF
AID
AIH
IVF explanation
In Vitro Fertilisation.
Artificial Insemination by Donor.
Artificial Insemination by Husband.
Egg cells are taken from the mother.... The egg is mixed with a…
IVF
In Vitro Fertilisation.
AID
Artificial Insemination by Donor.
11 terms
reproductive cloning
cloning
Hans Driesch
F.C Steward
Robert Briggs and Thomas J. King, nucl…
a process by which genetically identical copies of an organis…
1880, cloned sea urchins by separating two cells and growing…
1964, grew a complete carrot plant from a fully differentiate…
1952. cloned the northern leopard frog using nuclear transfer…
cloning
a process by which genetically identical copies of an organis…
Hans Driesch
1880, cloned sea urchins by separating two cells and growing…
16 terms
Animal Cloning
1st cloning experiment
Early Embryo
Embryo
Human Clones
Hans Driesh 1885
sperate cells into 4 individual cells
each genetical identical
identical twins (monozygotic)
1st cloning experiment
Hans Driesh 1885
Early Embryo
sperate cells into 4 individual cells
8 terms
bio cloning
selective breeding
Hybridization
Inbreeding
Gene spicing
picking the best seeds or animals and allowing them to breed
crossing two dissimilar individuals to bring together traits…
breeding individuals with similar traits
cuts DNA from one organism and puts it in another
selective breeding
picking the best seeds or animals and allowing them to breed
Hybridization
crossing two dissimilar individuals to bring together traits…
20 terms
Cloning Video
Are twins clones?
what year was Dolly the sheep cloned?
making a copy of an animal or person i…
therapeutic cloning tries to make spar…
yes
1997
reproductive
sick
Are twins clones?
yes
what year was Dolly the sheep cloned?
1997
8 terms
Cloning Mammals
What is cloning a type of?
How does cloning happen?
Shortage of organs
Understanding
Asexual reproduction as the cells are genetically identical.
A nucleus is enucleated from an adult body cell and is insert…
Cloning mammals e.g. pigs could help with the shortage of org…
The study of clones gives a greater understanding of the deve…
What is cloning a type of?
Asexual reproduction as the cells are genetically identical.
How does cloning happen?
A nucleus is enucleated from an adult body cell and is insert…
11 terms
Gcse cloning
Define clones
How do plants and bacteria produce clo…
Asexual reproduction:
Advantage of asexual reproduction
Individuals with identical genes
They reproduce asexually
One parent is involved so offspring has identical DNA
Individuals focus their energy on growing and reproducing tha…
Define clones
Individuals with identical genes
How do plants and bacteria produce clo…
They reproduce asexually
10 terms
Molecular Cloning
Molecular Cloning
Gene Cloning Main Steps
Restriction Endonuclease
DNA Ligase
Isolation and incorporation of a piece of DNA into a vector s…
1. Isolation and fragmentation of source DNA.... 2. Insertion of…
Enzyme that cuts DNA. It creates identical sticky ends on DNA…
Makes phosphodiester bonds and ligates the 2 ends of DNA frag…
Molecular Cloning
Isolation and incorporation of a piece of DNA into a vector s…
Gene Cloning Main Steps
1. Isolation and fragmentation of source DNA.... 2. Insertion of…
11 terms
Biotech Cloning
Enzymes important in DNA Cloning
Endonucleases are enzymes that break t…
Most useful type of endonuclease
What sequences do Type II endonuclease…
1. Type II Restriction Endonuclease... 2. DNA ligase... 3. DNA Poly…
Phosphodiester
Type II
Palindromic ( usually 4-6 bp in length)
Enzymes important in DNA Cloning
1. Type II Restriction Endonuclease... 2. DNA ligase... 3. DNA Poly…
Endonucleases are enzymes that break t…
Phosphodiester
10 terms
Cloning and genomics
Blastocyst
Embryonic stem cells
Adult stem cells
Creating induced pluripotent stem cells
The stage when the embryo is a hallow ball of cells ... Cells i…
Totipotent ... Easy to find... Cells from early stage embryos can…
Multi potent ... Difficult to isolate... Have to know how to indu…
Isolate skin or muscle cells (not stem cells)... Treat with rep…
Blastocyst
The stage when the embryo is a hallow ball of cells ... Cells i…
Embryonic stem cells
Totipotent ... Easy to find... Cells from early stage embryos can…
15 terms
Science Cloning
selective breeding
hybrids
another name for cloning
what happens a cloning
The process by which humans breed other animals or plants for…
The cross of animals results in what is called a crossbreed
mitosis
body cell nucleus is entering into egg cell
selective breeding
The process by which humans breed other animals or plants for…
hybrids
The cross of animals results in what is called a crossbreed
12 terms
Molecular Cloning
Ava I
CIP
DH5α
Eco RI
...
nonspecifically catalyzes the dephosphorylation of 5´ and 3´…
a strain of E. coli developed for laboratory cloning use.... clo…
is an endonuclease enzyme isolated from strains of E. coli, a…
Ava I
...
CIP
nonspecifically catalyzes the dephosphorylation of 5´ and 3´…
19 terms
DNA Cloning
Name two proteins that can be produced…
Purpose of DNA cloning?
What DNA ligase is usually used?
Describe the steps of CaCl2 transforma…
-Human insulin... -Human growth hormone
To produce a large number of identical DNA molecules in bacte…
T4 DNA ligase
• Cool it down to 4 degrees... • Add CaCl2 ... • Add plasmid... • Head…
Name two proteins that can be produced…
-Human insulin... -Human growth hormone
Purpose of DNA cloning?
To produce a large number of identical DNA molecules in bacte…
19 terms
Twinning and Cloning
Dizygotic/fraternal twins
Monozygotic/identical twins
Conjointe/Siamese twins
Mirror image twins
Twins that result when two oocytes are ovulated and both are…
Twins that arise from division of a single zygote. Division g…
Twins that are physically joined caused by incomplete separat…
Twins who are a mirror image of each other. External features…
Dizygotic/fraternal twins
Twins that result when two oocytes are ovulated and both are…
Monozygotic/identical twins
Twins that arise from division of a single zygote. Division g…
31 terms
Molecular Cloning
restriction endonucleases
Where were restriction enzymes discove…
Why do bacteria use restriction enzymes?
Restriction site
-cleave DNA at specific sequences... -allow long pieces of DNA t…
bacteria
to protect themselves from bacteria
where the restriction enzyme binds to and cuts a specific DNA…
restriction endonucleases
-cleave DNA at specific sequences... -allow long pieces of DNA t…
Where were restriction enzymes discove…
bacteria
18 terms
le clonage/ cloning
un but/ un objectif
un morceau
un problème éthique
un savant fou
aim
a piece
ethical issue
a mad scientist
un but/ un objectif
aim
un morceau
a piece
PGD and Cloning
Preimplantation genetic diagnosis
PGD procedure
PGD ethical issues
Human reproductive cloning
Identifies genetic defects in in vitro embryos before they ar…
Eggs taken from mother and fertilized in the lab, cells are e…
Murdering embryos if life starts at conception... Cannot guarant…
Creation of a person who has identical nuclear genetic materi…
Preimplantation genetic diagnosis
Identifies genetic defects in in vitro embryos before they ar…
PGD procedure
Eggs taken from mother and fertilized in the lab, cells are e…
6 terms
Therapeutic Cloning
Therapeutic Cloning
Reproductive Cloning
Multipotent Cells
Pluripotent Cells
The production of human embryos for use and research... The goal…
Technology used to generate in animal with the same nuclear D…
- Can develop into more than one cell type... - more limited tha…
- Can give rise to all the cell types that make up the body... -…
Therapeutic Cloning
The production of human embryos for use and research... The goal…
Reproductive Cloning
Technology used to generate in animal with the same nuclear D…
12 terms
Cloning and micropropogation
Transplanting a cell nucleus
Enucleated cell
Diploid nucleus, from a mature udder c…
To start dividing by mitosis as if it…
How is cloning an adult mammal done
The nucleus of a sheep's egg cell was removed
What is inserted into the enucleated cell
Why is the enucleated cell, containing a diploid nucleus, sti…
Transplanting a cell nucleus
How is cloning an adult mammal done
Enucleated cell
The nucleus of a sheep's egg cell was removed
21 terms
Twins and Cloning
Dizygotic twins/Fraternal twins
Monozygotic Twins/ Identical Twins
Conjoined/Siamese Twins
Mirror image twins
Twins that result when two oocytes are ovulated and both are…
Twins that arise from a division of a single zygote... Division…
Twins that are physically joined... Cause: incomplete separation…
Twins that are a mirror image of each other ... External feature…
Dizygotic twins/Fraternal twins
Twins that result when two oocytes are ovulated and both are…
Monozygotic Twins/ Identical Twins
Twins that arise from a division of a single zygote... Division…
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