Study sets matching "immunoglobulins"

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Study sets matching "immunoglobulins"

Immunoglobulins
What are the subtypes of Immunoglobuli…
What is the main immunoglobulin?
IgG
What are the 2 immunoglobulins that ca…
IgM... IgA... IgD... IgE... IgG
IgG- has all the functions
Delayed response, indicates a more chronic infection... =seconda…
IgG and IgM
What are the subtypes of Immunoglobuli…
IgM... IgA... IgD... IgE... IgG
What is the main immunoglobulin?
IgG- has all the functions
23 terms
Immunoglobulins
______ divides Igs into a F(ab')2 and…
Isotypes of Igs are determined by what?
What do allotypic differences in Igs u…
Idiotypic differences in Igs involve w…
Pepsin; Papain
Heavy chains (μ, γ, δ, α, ε).
Allotypic differences usually involve changes in one or more…
They're involved with the variability in the regions of the h…
______ divides Igs into a F(ab')2 and…
Pepsin; Papain
Isotypes of Igs are determined by what?
Heavy chains (μ, γ, δ, α, ε).
53 terms
Immunoglobulins
Roles for antibodies
IgG Subclasses
IgA Subclasses
Immunoglobulin domains
Immune response... Specific antisera... Monoclonal Antibodies... Diagn…
1,2,3,4
1,2,slgA
Constant... Variable
Roles for antibodies
Immune response... Specific antisera... Monoclonal Antibodies... Diagn…
IgG Subclasses
1,2,3,4
54 terms
Immunoglobulins
Roles for antibodies
IgG Subclasses
IgA Subclasses
Immunoglobulin domains
Immune response... Specific antisera... Monoclonal Antibodies... Diagn…
1,2,3,4
1,2,slgA
Constant... Variable
Roles for antibodies
Immune response... Specific antisera... Monoclonal Antibodies... Diagn…
IgG Subclasses
1,2,3,4
60 terms
Immunoglobulins
immunoglobulins is another word for
THEME of antibody role
Four chains of immunoglobulins
What links light and heavy chains
Antibodies
bind to toxins/antigens and enhance immune response which is…
2 Heavy and 2 light
Disulfide bridges; strong covalent bonds
immunoglobulins is another word for
Antibodies
THEME of antibody role
bind to toxins/antigens and enhance immune response which is…
16 terms
Immunoglobulins
Heavy chain
amino
hypervariable or CDR
covalent disulfide
this structural component of an Ig determines its isotype... com…
this terminal end is where the variable region is contained
found within the variable region of an Ig. This portion recog…
the type of bond that holds the four polypeptide chains of an…
Heavy chain
this structural component of an Ig determines its isotype... com…
amino
this terminal end is where the variable region is contained
Immunoglobulins
First expressed on cell surface and fi…
Most abundant on cell surface and seco…
Mononomer on cell surface but pentamer…
Activates complement (>IgG) ... Opsonizat…
IgM
IgM
IgM
IgM
First expressed on cell surface and fi…
IgM
Most abundant on cell surface and seco…
IgM
25 terms
Immunoglobulins
OVERVIEW
What is immunoglobulin
understand the significance of multipl…
9 IMPORTANT Ab PARTS - LIST
immunoglobulin = antibody... Ig = immunoglobulin - usually gener…
One of the initial ways to identify Igs was through gel elect…
OVERVIEW
What is immunoglobulin
immunoglobulin = antibody... Ig = immunoglobulin - usually gener…
8 terms
Immunoglobulins
Major class of circulation antibody
The pair of heavy chains & light chain…
Constant region
Variable region
IgG (gamma globulins)
Disulfide bridges
Region of IgG at C-terminal region of heavy and light chains…
Where binding of antigen occurs. Region of IgG at the N-termi…
Major class of circulation antibody
IgG (gamma globulins)
The pair of heavy chains & light chain…
Disulfide bridges
24 terms
Immunoglobulins
IgG
IgM
IgA
IgD
- MW: 150,000... - 7S... - Valence: 2... - Half life: 23... - Crosses pla…
- MW: 900,000... - "macroglobulins"... - 19S... - Valence: 10 (5 reali…
- MW: serum - 160,000... secretory - 380,000... - serum: 7S... secreto…
- MW: 180,000... - 7S... - Valence: 2... - Half life: 2.8... - Complement…
IgG
- MW: 150,000... - 7S... - Valence: 2... - Half life: 23... - Crosses pla…
IgM
- MW: 900,000... - "macroglobulins"... - 19S... - Valence: 10 (5 reali…
Immunoglobulins
Antibodies
Three Types of Globulins
Antibodies are ___ globulins.
Polyclonal Antibodies
Antigen specific proteins produced by plasma cells that belon…
1.) Alpha ... 2.) Beta... 3.) Gamma
gamma
Secreted by different B-cell lineages within the body. (Heter…
Antibodies
Antigen specific proteins produced by plasma cells that belon…
Three Types of Globulins
1.) Alpha ... 2.) Beta... 3.) Gamma
26 terms
Immunoglobulins
Antibody... IgG most utilized in IHC
Light chain
Heavy chain
gamma, alpha, mu, delta, epsilon
are made by a class of white blood cells, called B lymphocyte…
Makes up 2 identical outermost chains on the antibody... 2 type…
-2 identical innermost chains... - differ in antigenic and stru…
What are the five heavy chains that make up the different iso…
Antibody... IgG most utilized in IHC
are made by a class of white blood cells, called B lymphocyte…
Light chain
Makes up 2 identical outermost chains on the antibody... 2 type…
97 terms
Immunoglobulins
Infliximab
Omalizumab
Primary defense against
Effector mechanisms
Anti-TNF Ab
Anti-IgE Ab... Think: bind to IgE to prevent allergic reaction…
EXTRACELLULAR organisms... Ab bind to microbial Ag, Neutralize,…
promote phagocytosis (opsonin-- IgG C3b)... Inflammatory mediato…
Infliximab
Anti-TNF Ab
Omalizumab
Anti-IgE Ab... Think: bind to IgE to prevent allergic reaction…
Immunoglobulins
IgG
IgG
IgA
IgM
Major line of defense
Slowest produced immunoglobulin
Found in exocrine gland secretions, such as tears, breast mil…
First responder, produced quickest
IgG
Major line of defense
IgG
Slowest produced immunoglobulin
224 terms
Immunoglobulins
Antibody basic structure
Ig domain
Fab region + parts that make it up
Fc region + parts that make it up
-2 identical light chains... -2 identical heavy chains
Type of protein domain (pattern) that consists of a 2-layer s…
Hypervariable region of Ig where antigens bind; VH, VL, CH1, CL
Constant region of Ig that confers the effector function; CH2…
Antibody basic structure
-2 identical light chains... -2 identical heavy chains
Ig domain
Type of protein domain (pattern) that consists of a 2-layer s…
48 terms
Immunoglobulins
What is the common origin of all cells…
What is another name for a surface imm…
What is another name for a (free) immu…
Which cells express surface immunoglob…
Hematopoietic stem cell, common lymphoid progenitor
B-cell receptor
Antibody
B cells
What is the common origin of all cells…
Hematopoietic stem cell, common lymphoid progenitor
What is another name for a surface imm…
B-cell receptor
Immunoglobulins
IgG1
IgG2
IgG3
IgG4
Heavy chain g1, MW = 146 kDa... 9 mg/ml in serum, 21 day half-li…
Heavy chain g2, MW = 146 kDa... 1 mg/ml in serum, 7 day half-lif…
Heavy chain g3, MW = 170 kDa... 3 mg/ml in serum, 20 day half-li…
Heavy chain g4, MW = 146 kDa... 0.5 mg/ml in serum, 21 day half-…
IgG1
Heavy chain g1, MW = 146 kDa... 9 mg/ml in serum, 21 day half-li…
IgG2
Heavy chain g2, MW = 146 kDa... 1 mg/ml in serum, 7 day half-lif…
22 terms
Immunoglobulins
Describe features of innate immune sys…
Describe features of adaptive immune s…
Antibodies are an example of what type…
Immunoglobulins bind to what and how s…
1. non-specific, no memory... 2. first line = physical and chemi…
1. specific and acquire memory... 2. antibody response... 3. T cell…
Adaptive
Antigens... very strongly = dissociation constant Kd 10-7
Describe features of innate immune sys…
1. non-specific, no memory... 2. first line = physical and chemi…
Describe features of adaptive immune s…
1. specific and acquire memory... 2. antibody response... 3. T cell…
5 terms
Immunoglobulins
IGE
IGM
IGA
IGG
In response to an allergy
In response to an active infection
Found in tears, saliva, and colostrum
Shows past infection and immunity
IGE
In response to an allergy
IGM
In response to an active infection
29 terms
Immunoglobulins
Albumin
Antigen
Immunogen
Antigenic determinant, epitope
Most abundant plasma protein. Maintinas osmotic pressure, imp…
Any foreign molecule that is bound selectively by antibodies
The molecule that induces antibody production. May be a prote…
Small region of a larger molecule that elicits the production…
Albumin
Most abundant plasma protein. Maintinas osmotic pressure, imp…
Antigen
Any foreign molecule that is bound selectively by antibodies
64 terms
Immunoglobulins
What are the two types of immunoglobul…
Run me from BCR to secrted Ab
How many antigens can an Ab bind to?
How many kDA is a antibody?
BCR... secreted Ab
1. Expressed on mature B cells in a membrane-bound form that…
Just one
150 kDa
What are the two types of immunoglobul…
BCR... secreted Ab
Run me from BCR to secrted Ab
1. Expressed on mature B cells in a membrane-bound form that…
72 terms
Immunoglobulins
antibody
antigen
epitope
positive and negative effects of antib…
secreted form of the B cell receptor (BCR), also referred to…
specific molecule(s) of a pathogen to which an immune respons…
specific part or feature of an antigen which is recognized by…
positive: ... neutralize toxins or viruses... aid in uptake and cle…
antibody
secreted form of the B cell receptor (BCR), also referred to…
antigen
specific molecule(s) of a pathogen to which an immune respons…
24 terms
Immunoglobulins
What is the immune system?
What is the immune system divided into…
Describe the features of the innate im…
Describe the features of the adaptive…
Is a complex network of cells and soluble molecules which int…
The innate & adaptive system
- Non-specific ... - No memory... - First line: physical & chemical…
- Specific ... - Acquisition of memory ... - Antibody response... - T-…
What is the immune system?
Is a complex network of cells and soluble molecules which int…
What is the immune system divided into…
The innate & adaptive system
20 terms
Immunoglobulins
isotypes
idiotype
multimeric antibodies
Papain
determined by the constant regions of the heavy chains
serves as a clonal marker for an immunoglobulin molecule and…
IgM (pentameric) and IgA (can be monomeric or dimeric)... -J-cha…
cleaves Ig N-terminal side of the hinge region to create 2 id…
isotypes
determined by the constant regions of the heavy chains
idiotype
serves as a clonal marker for an immunoglobulin molecule and…
immunoglobulins
IgG
IgM
IgA
IgD
blood
first to increase immune response
found in secretions such as tears, spit, colostrum
B cell receptor
IgG
blood
IgM
first to increase immune response
5 terms
Immunoglobulins
IgM
IgA
IgD
IgG
Largest amount, appear at first exposure
Usually within secretions
Basophil binding, smallest amount
Second response to an infection
IgM
Largest amount, appear at first exposure
IgA
Usually within secretions
5 terms
Immunoglobulins
IgM
IgG
IgA
IgD
first Ig class, produced after initial exposure to the antige…
monomer most abundant in the blood, tissue fluids, and can cr…
present in secretions in tears, saliva, mucus, and breast milk
present in surface of naive b cells
IgM
first Ig class, produced after initial exposure to the antige…
IgG
monomer most abundant in the blood, tissue fluids, and can cr…
Immunoglobulins
IgG
IgA
IgM
IgD
Appears in serum and tissues... Assumes major role in bloodborne…
Appears in body fluids... Protect against respiratory, GI, and g…
Appears mostly in intravascular serum... Appears as first immung…
Appears in small ammounts in serum... Possibly influences B-lymp…
IgG
Appears in serum and tissues... Assumes major role in bloodborne…
IgA
Appears in body fluids... Protect against respiratory, GI, and g…
5 terms
Immunoglobulins
IgM
IgE
IgG
IgA
Primary immune response
Stimulates release of Histamine from Mast Cells/Basophils
Secondary Immune Response; Half Life=23 days
Body secretions
IgM
Primary immune response
IgE
Stimulates release of Histamine from Mast Cells/Basophils
immunoglobulins
IgG
IgA
IgM
IgD
Most abundant, about 80% of all antibodies in blood; found in…
Found mainly in sweat, tears, saliva, mucus, breast milk, and…
About 5-10% of all antibodies in blood; also found in lymph.…
Mainly found on surfaces of B cells as antigen receptors, whe…
IgG
Most abundant, about 80% of all antibodies in blood; found in…
IgA
Found mainly in sweat, tears, saliva, mucus, breast milk, and…
immunoglobulins
antiviral, antitoxin and antibacterial…
in body secretions like saliva, nasal…
forms natural antibodies for the blood
important in maturation of B lymphocytes
IgG
IgA
IgM
IgD
antiviral, antitoxin and antibacterial…
IgG
in body secretions like saliva, nasal…
IgA
Immunoglobulins
Immunoglobulins: most abundant to leas…
IgG
IgA
IgM
IgG.... IgA.... IgM.... IgD.... IgE.
639 to 1,349 mg/dl. ... (usually about 75% of total)... only imm…
70 to 312 mg/dl. ... (10-15%)... Second most common immunoglobul…
86 to 352 mg/dl.... (7-10%)... Does not cross the placenta. ... IgM…
Immunoglobulins: most abundant to leas…
IgG.... IgA.... IgM.... IgD.... IgE.
IgG
639 to 1,349 mg/dl. ... (usually about 75% of total)... only imm…
5 terms
Immunoglobulins
IgG
IgA
IgM
IgE
major in blood, opsonization, neutralization via complement
...
...
...
IgG
major in blood, opsonization, neutralization via complement
IgA
...
20 terms
immunoglobulins
IgD
IgE
IgG
IgA
No known serum value; 1 of 2 BCR types
Reaginic antibody; Type 1 Hypersensitivity Reactions; Protect…
Immunity for Pathogens; Best immunity antigen; Toxin, Bacteri…
Secretory-Breast milk; J-chain----Serum-Neutralizing and oxid…
IgD
No known serum value; 1 of 2 BCR types
IgE
Reaginic antibody; Type 1 Hypersensitivity Reactions; Protect…
7 terms
Immunoglobulins
Immunoglobulin
Opsonin
IgA
IgG
Antibody
Molecule that enhances phagocytosis by marking antigen for im…
Saliva, tears, blood, protect body surfaces exposed to outsid…
Most common type of antibody, ~75% of serum antibodies, creat…
Immunoglobulin
Antibody
Opsonin
Molecule that enhances phagocytosis by marking antigen for im…
Immunoglobulins
IgG
IgA
IgM
IgD
Activates complement
Found in exocrine secretions
Anti-a, -b, -rh
Found on B cells
IgG
Activates complement
IgA
Found in exocrine secretions
Immunoglobulins
What are immunoglobulins?
B cells
Plasma (effector) cells
3 secondary response characteristics f…
The antigen recognition molecules of B cells
> Differentiate into Plasma cells (effector cells)... > Differen…
Secrete antibodies which flag cells to be destroyed by Macrop…
> Produce antibodies which bind with greater affinity to thei…
What are immunoglobulins?
The antigen recognition molecules of B cells
B cells
> Differentiate into Plasma cells (effector cells)... > Differen…
8 terms
Immunoglobulins
IgG
IgM
IgA
IgE
Opsonization of antigens for phagocytosis by macrophages and…
Activation of the classical pathway of complement... Antigen rec…
Mucosal immunity: secretion of IgA into the lumens of GI and…
IgE Mast cell degranulation (immediate hypersensitivity react…
IgG
Opsonization of antigens for phagocytosis by macrophages and…
IgM
Activation of the classical pathway of complement... Antigen rec…
5 terms
Immunoglobulins
IgA
IgD
IgE
IgM
Present in body secretions, tears, saliva, mucus. 10%
Present in B cells... B cells to plasma cells.
Present in basophils, releases heparin & histamine during inf…
Produced by plasma cells, 1st time encountering pathogen... 1st…
IgA
Present in body secretions, tears, saliva, mucus. 10%
IgD
Present in B cells... B cells to plasma cells.
Immunoglobulins
IgG
IgM
IgA
IgE
Secondary response antibody. Cross the placenta. Most common…
Primary response antibody. First made in response to antigen
Body secretions, secretory component, highest amount in body
Allergies, parasitic worms, binds to mast cells to release hi…
IgG
Secondary response antibody. Cross the placenta. Most common…
IgM
Primary response antibody. First made in response to antigen
Immunoglobulins
IgD
IgE
IgA
IgG
Antigen-r on naive follicular B cells
Activation of mast cells and basophils to promote barrier imm…
Neutralization, protection of mucosa, induction of phagocytos…
Neutralization, complement activation, induction of phagocyto…
IgD
Antigen-r on naive follicular B cells
IgE
Activation of mast cells and basophils to promote barrier imm…
immunoglobulins
IgA
IgD
IgE
IgG
blood plasma, mucus, tears, saliva, interstitial secretions... p…
acts as receptor for B cells... activation of B cells by antigens
associated with basophils and mast cells, eosinophils and par…
most abundant... involved in secondary immune response... can cross…
IgA
blood plasma, mucus, tears, saliva, interstitial secretions... p…
IgD
acts as receptor for B cells... activation of B cells by antigens
5 terms
Immunoglobulins
IgG
IgM
IgA
IgD
They are found in all body fluids, the function is it protect…
This is the first one to reach an infection, B cells activate…
They are found in high concentrations in the mucous membranes…
This is found on the surface of the B cells and are found on…
IgG
They are found in all body fluids, the function is it protect…
IgM
This is the first one to reach an infection, B cells activate…
Immunoglobulins
IgG
IgG
IgA
IgM
only antibody that can be transferred through the placenta
most abundant antibody
predominant antibody in secretions (saliva, tears, milk, mucu…
second most abundant antibody
IgG
only antibody that can be transferred through the placenta
IgG
most abundant antibody
Immunoglobulins
Whole blood... Serum... Plasma... CSF... Serous fl…
10-15% lower
30 mins - 1 hr
2hrs
Samples for glucose determination
Whole blood gives approximately _______ glucose levels than s…
A serum specimen is appropriate for glucose analysis if serun…
Maximum of hours specimen should be kept at room temp
Whole blood... Serum... Plasma... CSF... Serous fl…
Samples for glucose determination
10-15% lower
Whole blood gives approximately _______ glucose levels than s…
10 terms
Immunoglobulins
IgM
IgA
IgD
IgG
Initial attack. Good at clumping microorganisms for elimination
Secretions and mucosal immunity. Stimulated by IN vaccinations
Abundance on B cell membranes. Low levels in serum. Activate…
Most abundant. Can cross placenta
IgM
Initial attack. Good at clumping microorganisms for elimination
IgA
Secretions and mucosal immunity. Stimulated by IN vaccinations
5 terms
Immunoglobulins
appears in serum and tissues (intersti…
appears in body fluids (blood, saliva,…
appears mostly in intravascular system…
appears in small amounts in serum; pos…
IgG
IgA
IgM
IgD
appears in serum and tissues (intersti…
IgG
appears in body fluids (blood, saliva,…
IgA
Immunoglobulins
igA
igA
igA
igA
combines with protein in mucosa to protect body surfaces from…
2nd largest class of immunoglobins
found in tears, saliva, sweat, mucus membreanes and bile
it is secretes by plama cells in the GI and resp. tracts and…
igA
combines with protein in mucosa to protect body surfaces from…
igA
2nd largest class of immunoglobins
Immunoglobulins
IgM
IgG
IgA
IgE
Largest antibody... Produced when an animal is first exposed to…
Smallest and most common... Produced and released by plasma cells
Protects body surface from foreign substances
Bonds to allergens and triggers histamine release from mast c…
IgM
Largest antibody... Produced when an animal is first exposed to…
IgG
Smallest and most common... Produced and released by plasma cells
6 terms
Immunoglobulins
found in nasal secretions, SALIVA, and…
causes degranulation of mast cells whi…
enhances phagocytosis; found in EXUDAT…
largest in size; triggers IgG; activat…
IgA
IgE
IgG
IgM
found in nasal secretions, SALIVA, and…
IgA
causes degranulation of mast cells whi…
IgE
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