Study sets matching "microbiology 3 microscopy"

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Study sets matching "microbiology 3 microscopy"

Microbiology Ch 3 (Microscopy)
Micrometer
Nanometer
Simple Microscope
Compound Microscope
10^-6m
10^-9m
One lens
Image from objective lens is magnified again by the ocular lens
Micrometer
10^-6m
Nanometer
10^-9m
24 terms
Microbiology Ch. 3: Microscopy
simple microscope
compound microscope
total magnification
maximum resolution and magnification i…
uses one lens
has multiple lens
magnification of objective x magnification of ocular
0.2 um ... 2000x
simple microscope
uses one lens
compound microscope
has multiple lens
50 terms
Chapter 3 Microbiology- Microscopy
Historical Roots of Microbiology
What did Anthony Van Leuwenhoek's micr…
Robert Hook credited with the fist boo…
Refraction
Although the existence of microbes too small to be seen with…
Discovering microorganisms
Microscope observations and structures in plants and animals.
Light bends when it passes from one medium slowly into a dens…
Historical Roots of Microbiology
Although the existence of microbes too small to be seen with…
What did Anthony Van Leuwenhoek's micr…
Discovering microorganisms
15 terms
Microbiology Chapter 3: Microscopy
light microscope
electron microscope
atomic force microscope
x-ray crystallography
can magnify 1000x, common, important tool in microbiology
can magnify more than 100,000x
can produce images of individual atoms on a surface (ex. plas…
not a microscope, can resolve protein structures at the atomi…
light microscope
can magnify 1000x, common, important tool in microbiology
electron microscope
can magnify more than 100,000x
12 terms
Microbiology Ch 3 - Microscopy
Mention the light microscopes
Mention the electron microscopes
Which microscopes are useful to observ…
Which microscopes provide 3D images?
Brightfield, darkfield, DIC, Fluorescence, phase contrast
Transmission (TEM) and scanning (SEM)
brightfield, darkfield, phase-contrast
confocal, DIC, SEM, phase-contrast
Mention the light microscopes
Brightfield, darkfield, DIC, Fluorescence, phase contrast
Mention the electron microscopes
Transmission (TEM) and scanning (SEM)
30 terms
Microbiology: Chapter 3: Microscopy
Nanometers
Micrometers
Millimeters
how to convert
10^-9, nm
10^-6, um
10^-3, mm
o If you are converting to a SMALLER unit, move decimal to th…
Nanometers
10^-9, nm
Micrometers
10^-6, um
22 terms
Microbiology Chapter 3 Microscopy
Parts of Compound Microscope & Function
Magnification
How to Calculate magnification of a sp…
Resolution
image from the objective lens is magnified by the ocular lens…
An increase in the apparent size of a viewed object as an opt…
total magnification = objective lens x ocular lens
the ability of the lenses to distinguish two points... The smal…
Parts of Compound Microscope & Function
image from the objective lens is magnified by the ocular lens…
Magnification
An increase in the apparent size of a viewed object as an opt…
Microbiology packet 3 (microscopy)
Resolving power
Bright field microscopy
Dark field microscopy
Fluorescence Microscope
Ability to distinguish separate adjacent objects. resolution…
- simplest, cheap ... - usually requires fixed, dry, and dead sp…
- special condenser... - background black, specimen lights up ... -…
...
Resolving power
Ability to distinguish separate adjacent objects. resolution…
Bright field microscopy
- simplest, cheap ... - usually requires fixed, dry, and dead sp…
90 terms
Microbiology Chapter 3 Microscopy
1 um=
Resolution
Shorter wavelengths provide
Refractive index
1000 nm
Is the ability of the lenses to distinguish between two points
Greater resolution
Is a measure of the light bending ability of a medium
1 um=
1000 nm
Resolution
Is the ability of the lenses to distinguish between two points
24 terms
Microbiology: Lecture 3 (Microscopy)
What is the size range of eukaryotic m…
What is the size range of prokaryotes?
Resolution
Detection
10-100 micrometers
0.4-10 micrometers
The smallest distance by which two objects can be separated a…
The ability to determine the presence of an object
What is the size range of eukaryotic m…
10-100 micrometers
What is the size range of prokaryotes?
0.4-10 micrometers
15 terms
Microbiology Chapter 3:Microscopy
compound light microscope
illumination
condenser
ocular lenses
uses visible light to illuminate samples
a light bulb located at the base of the microscope
concentrate the light rays passing through the specimen... locat…
magnify the sample about 10 times
compound light microscope
uses visible light to illuminate samples
illumination
a light bulb located at the base of the microscope
18 terms
Microbiology Chapter 3 Microscopy
Total Magnification
Resolution
dark objects are visible against a bri…
Uses UV light and makes the structure…
objective lens*ocular lens
the ability of the lenses to distinguish two points
Bright-Field Microscope
Fluorescence Microscope
Total Magnification
objective lens*ocular lens
Resolution
the ability of the lenses to distinguish two points
Chapter 3 Microbiology/ Microscopy
TOPIC #1 PRINCIPLES OF MICROSCOPY
1. WHAT IS ANTON LEEUWENHOEK'S CONTRIB…
2. EXPLAIN THE RELATIONSHIP OF (METER,…
TOPIC #2 PROPERTIES OF LIGHT-- WAVELEN…
...
-IMPROVED THE DEVELOPMENT OF MICROSCOPES... -DISCOVERED SINGLE C…
METER-... MILLIMETER-... MICROMETER- equal to 0.000001m or 10⁻⁶ a…
...
TOPIC #1 PRINCIPLES OF MICROSCOPY
...
1. WHAT IS ANTON LEEUWENHOEK'S CONTRIB…
-IMPROVED THE DEVELOPMENT OF MICROSCOPES... -DISCOVERED SINGLE C…
MICROBIOLOGY CH3 MICROSCOPY
MAGNIFICATION
PARFOCAL
RESOLUTION (POWER)
REFRACTIVE INDEX
The enlargement of a picture not the size of the microbe.
Stay in focus when changing magnification.
Ability to distinguish fine detail and structure.
Velocity in which light passes through a substance.
MAGNIFICATION
The enlargement of a picture not the size of the microbe.
PARFOCAL
Stay in focus when changing magnification.
24 terms
Microbiology Lecture 3 (microscopy)
What units are used to measure microor…
What parts does a compound light micro…
Define total magnification
Define resolution
Micrometers and nanometers
STUDY DIAGRAM OF THE MICROSCOPE IN POWERPOINT
objective lens x ocular lens
Ability of the lenses to distinguish two points that are a sp…
What units are used to measure microor…
Micrometers and nanometers
What parts does a compound light micro…
STUDY DIAGRAM OF THE MICROSCOPE IN POWERPOINT
Microbiology Chapter 3 (Microscopy &staining)
Bight-field illumination
Dark-field illumination
Phase-contrast
Confocal Microscopy
when the condenser uses ordinary light, to be concentrated an…
(used for organism sensitive to light) a special condenser th…
(used to observe alive and unstained organism)
Uses beams of ultraviolet laser light to excite fluorescent c…
Bight-field illumination
when the condenser uses ordinary light, to be concentrated an…
Dark-field illumination
(used for organism sensitive to light) a special condenser th…
13 terms
Microbiology Lecture 3 Microscopy and Classification
What two factors are important in micr…
List the 3 types of light microscopy.…
What type of light does fluorescent mi…
What are the 2 major types of electron…
Resolution- clearer image... Magnification- Larger image
Bright field... Dark field... Phase contrast... - Bright light is most…
-UV light... - stains are used
-Transmission and Scanning... -Transmission allows you to see in…
What two factors are important in micr…
Resolution- clearer image... Magnification- Larger image
List the 3 types of light microscopy.…
Bright field... Dark field... Phase contrast... - Bright light is most…
41 terms
Microbiology Chapter 3: Microscopy and Staining
kilometer
meter
decimeter
centimeter
1000
standard unit of metric length
.10
.01
kilometer
1000
meter
standard unit of metric length
66 terms
Microbiology Chapter 3 - Microscopy & Staining
Who was the first person to see indivi…
Microscopy
Wavelength
Resolution
van Leeuwenhoek - constructed simple microscopes capable of m…
The technology of making very small things visible to the hum…
The length of a light ray. The distance between two adjacent…
Refers to the ability to see two items as separate and discre…
Who was the first person to see indivi…
van Leeuwenhoek - constructed simple microscopes capable of m…
Microscopy
The technology of making very small things visible to the hum…
61 terms
Microbiology Chapter 3: Microscopy and Staining
Leeuwenhock
microscopy
wavelength
white light
- first person to see individual organisms... - built a new micr…
the technology of making very small things visible to the eye
- the length of a light ray... - one of the most important prope…
the combination of all colors of visible light
Leeuwenhock
- first person to see individual organisms... - built a new micr…
microscopy
the technology of making very small things visible to the eye
62 terms
Microbiology Chapter 3 - Microscopy & Metric Units
What is Resolving Power?
What are the major parts of a Microsco…
What is the Coarse Focusing Knob?
What is a Illuminator?
Ability to see fine details of a specimen, or the maximum dis…
Base, Fine Focusing Knob, Coarse focusing knob, Illuminator,…
Moves the stage of the Microscope up and down to focus the im…
The light source for the Microscope.
What is Resolving Power?
Ability to see fine details of a specimen, or the maximum dis…
What are the major parts of a Microsco…
Base, Fine Focusing Knob, Coarse focusing knob, Illuminator,…
Microbiology - Microscopy
Resolution
Numerical aperature
Parfocal
Compound microscope
The ability of the microscope to separate small detail (resol…
The light gathering ability of the microscope
Sets of objectives or eyepieces that may be interchanged with…
Microscope made up of a series of lenses and an energy source…
Resolution
The ability of the microscope to separate small detail (resol…
Numerical aperature
The light gathering ability of the microscope
18 terms
Microbiology Lecture 3 - Microscopy and Staining
General Principles of Microscopy
Contrast
Light Microscopy... - Bright-field micros…
Light Microscopy... - Dark-field microsco…
- Wavelength of radiation... - Magnification... - Resolution... - Cont…
Differences in intensity between two objects, or... between an o…
• Simple... - Contain a single magnifying lens ... - Leeuwenhoek…
• Best for observing pale objects... • Specimen appears light ag…
General Principles of Microscopy
- Wavelength of radiation... - Magnification... - Resolution... - Cont…
Contrast
Differences in intensity between two objects, or... between an o…
18 terms
Microbiology - Microscopy
magnification
resolution
reflection
transmission
An increase in the apparent size of an object
-the clarity of a magnified image... -the ability to see two poi…
The amount of light being bounced back
The passing of light through matter
magnification
An increase in the apparent size of an object
resolution
-the clarity of a magnified image... -the ability to see two poi…
22 terms
Microbiology Chapter 1(people)&3(Microscopy and staining)
Robert Hooke
Louis Pasteur
Robert Koch
The Cell theory
coined the term "cell"
- cholera, rabies
-used techniques to isolate the bacterium that caused tubercu…
all cells are the fundamental units of life and carry out all…
Robert Hooke
coined the term "cell"
Louis Pasteur
- cholera, rabies
18 terms
Microbiology Ch 3 Microscopy Methods
What is microbial investigation?
Ok time for some vocab.. ... Colony
Culture
Subculture
Most environmental samples do not contain an isolated microor…
A macroscopic cluster of cells appearing on a solid medium, e…
The visible accumulation of microorganisms in or on a nutrien…
A pure colony used to grow a new batch of pure culture.
What is microbial investigation?
Most environmental samples do not contain an isolated microor…
Ok time for some vocab.. ... Colony
A macroscopic cluster of cells appearing on a solid medium, e…
Microbiology: Microscopy
What is light microscopy?
What are the four types of light micro…
What is the ocular lens?
What is the body tube of a microscope?
Any kind of microscope that uses visible light to observe spe…
-Compound light microscopy (should be able to see a really br…
Remagnifies the... image formed by... the objective lens
Transmits... the image from the... objective lens to the... ocular lens
What is light microscopy?
Any kind of microscope that uses visible light to observe spe…
What are the four types of light micro…
-Compound light microscopy (should be able to see a really br…
15 terms
Microbiology - Microscopy
Units of lenght
Different types of microscope used
Types of Light Microssopes
Brightfield Microscopy (problems)
1mm=1000um, 1um=1000nm, 1nm=10A... Virus=10-400nm... Bacteria 1-2um…
1. Light or optical microscope... 2. Electron microscope... 3. Scan…
1. Brightfield Microscopy... 2. Darkfield Microscopy... 3. Phase Co…
Contrast - water filled cells with water background... Magnifica…
Units of lenght
1mm=1000um, 1um=1000nm, 1nm=10A... Virus=10-400nm... Bacteria 1-2um…
Different types of microscope used
1. Light or optical microscope... 2. Electron microscope... 3. Scan…
18 terms
Microbiology Ch 3 Microscopy tools
What is the size of the average bacter…
What are the key characteristics of a…
Magnification occurs in two phases
Total Magnification
Bacteria are .5- 2 microns in size. Archaea are .5 microns to…
1. Magnification- ability to enlarge objects. 2. Resolution-…
First the objective lens forms the real image, then the real…
is a product of the separate magnifying powers of the two len…
What is the size of the average bacter…
Bacteria are .5- 2 microns in size. Archaea are .5 microns to…
What are the key characteristics of a…
1. Magnification- ability to enlarge objects. 2. Resolution-…
53 terms
Microbiology Chapter 3: Microscopy and Cell Structure
3 parameters of microscopy
Light Microscopy
Light Microscopy microscopes
Bright-field Microscope
-Magnification... -Resolution... -Contrast
Visible light passes through a series of lenses to produce a…
-Bright-field... -Dark-field... -Phase-contrast
Most common, illuminates field evenly... -stain to increase cont…
3 parameters of microscopy
-Magnification... -Resolution... -Contrast
Light Microscopy
Visible light passes through a series of lenses to produce a…
29 terms
Microscopy (Microbiology)
first to describe microorganisms visua…
naked eye, light microscope, electron…
3 types of light microscopy
3 lenses on light microscope
Anton van Leeuwenhoek
mm, um, nm
Brightfield, phase contrast, darkfield
condenser, objective lens, ocular lens
first to describe microorganisms visua…
Anton van Leeuwenhoek
naked eye, light microscope, electron…
mm, um, nm
Microbiology. Chapter 3. Microscopy & Staining
The compound light microscope can be u…
What is resolution, and why is it impo…
(a) : when light strikes an object and…
In light microscopes, what function do…
(e) Bacteria, cell organelles, and red blood cells
The ability to see two items as separate and discrete units r…
(a) reflection: when light strikes an object and bounces back…
(c) focuses light rays on the sample
The compound light microscope can be u…
(e) Bacteria, cell organelles, and red blood cells
What is resolution, and why is it impo…
The ability to see two items as separate and discrete units r…
51 terms
Microbiology - Microscopy & Cell Structure (Ch.3)
Differential Stains and Agars
Consists of LPS
Contains a thick layer of peptidoglycan
Mitochondria and Chloroplasts..
Acid-fast... Gram... Mannitol salt agar (MSA)... Eosin-methylene blue…
Endotoxin
Cell wall of Gram-positive bacteria
are capable of performing protein synthese... contain DNA and 70…
Differential Stains and Agars
Acid-fast... Gram... Mannitol salt agar (MSA)... Eosin-methylene blue…
Consists of LPS
Endotoxin
24 terms
Microbiology-- Chapter 3-- Microscopy & Staining
Bacteria with coccus morphology
Bacteria will bacillus morphology
Bacteria with coccobacillus morphology
Bacteria with vibrio morphology
Staphylococcus aureus -- MRSA... Streptococcus pyogenes -- cause…
Escherichia coli... Bacillus subtilis... Bacillus anthracis -- caus…
Bordetella pertussis -- causes whooping cough... Haemophilus inf…
Vibrio cholerae -- causes cholera
Bacteria with coccus morphology
Staphylococcus aureus -- MRSA... Streptococcus pyogenes -- cause…
Bacteria will bacillus morphology
Escherichia coli... Bacillus subtilis... Bacillus anthracis -- caus…
Microbiology Ch. 3 - Cell Structure and Microscopy
_______ do NOT have a membrane bound n…
What cell type have antibiotics to tar…
Are eukaryotes or Prokaryotes more com…
Bacteria is a ________ and is _________.
Prokaryotes
Eukaryotic cells
Eukaryotes
Prokaryote and is unicellular
_______ do NOT have a membrane bound n…
Prokaryotes
What cell type have antibiotics to tar…
Eukaryotic cells
110 terms
Microbiology; Microscopy
Which of the following is NOT a macron…
What micronutrient is essential for ba…
Algae are photosynthetic and get Carbo…
Obligative anaerobes can not use?
D. Silver
Iron
Photoautotroph
molecular Oxygen
Which of the following is NOT a macron…
D. Silver
What micronutrient is essential for ba…
Iron
62 terms
Microbiology Chapter 3 - Microscopy & Metric Units
What is Resolving Power?
What are the major parts of a Microsco…
What is the Coarse Focusing Knob?
What is a Illuminator?
Ability to see fine details of a specimen, or the maximum dis…
Base, Fine Focusing Knob, Coarse focusing knob, Illuminator,…
Moves the stage of the Microscope up and down to focus the im…
The light source for the Microscope.
What is Resolving Power?
Ability to see fine details of a specimen, or the maximum dis…
What are the major parts of a Microsco…
Base, Fine Focusing Knob, Coarse focusing knob, Illuminator,…
Microbiology Chapter 3 - Microscopy and Cell Structure
The Scanning Probe microscope
Magnification
Resolution
Refractive Index
Discovered in 1961
equals "Enlargement"
Distinguishes fine detail
Measure of a relative velocity of light as it travels through…
The Scanning Probe microscope
Discovered in 1961
Magnification
equals "Enlargement"
Microbiology Chapter 3 - Microscopy & Metric Units
What is Resolving Power?
What are the major parts of a Microsco…
What is the Coarse Focusing Knob?
What is a Illuminator?
Ability to see fine details of a specimen, or the maximum dis…
Base, Fine Focusing Knob, Coarse focusing knob, Illuminator,…
Moves the stage of the Microscope up and down to focus the im…
The light source for the Microscope.
What is Resolving Power?
Ability to see fine details of a specimen, or the maximum dis…
What are the major parts of a Microsco…
Base, Fine Focusing Knob, Coarse focusing knob, Illuminator,…
14 terms
Microbiology: CH 3: Microscopy and cell structure
Capsule
Chemotaxis
cytoplasmic membrane
Endospore
a distinct thick gelatinous material that surrounds some micr…
movement of a cell toward or away from a certain chemical in…
a ph0spholipid bi layer embedded with proteins that surround…
an extraordinarily resistant dormant cell produced by some ba…
Capsule
a distinct thick gelatinous material that surrounds some micr…
Chemotaxis
movement of a cell toward or away from a certain chemical in…
Microbiology - Microscopy
Simple microscope
Compound microscope
Magnification
Resolution
a microscope that has one lens
a microscope that has more than one lens
In compound microscopes, determined by the ocular lens and th…
the ability to distinguish 2 images as separate. e.g. our mic…
Simple microscope
a microscope that has one lens
Compound microscope
a microscope that has more than one lens
62 terms
Microbiology Chapter 3 - Microscopy & Metric Units
What is Resolving Power?
What are the major parts of a Microsco…
What is the Coarse Focusing Knob?
What is a Illuminator?
Ability to see fine details of a specimen, or the maximum dis…
Base, Fine Focusing Knob, Coarse focusing knob, Illuminator,…
Moves the stage of the Microscope up and down to focus the im…
The light source for the Microscope.
What is Resolving Power?
Ability to see fine details of a specimen, or the maximum dis…
What are the major parts of a Microsco…
Base, Fine Focusing Knob, Coarse focusing knob, Illuminator,…
51 terms
Microbiology-Microscopy
How is light refracted (bent)?
What is the refractive index?
What does refraction do?
What does reflection do?
When light passes from one medium to another.
A measure of how greatly a substance slows the speed of light.
It changes the direction of light.
It makes light bounce off of it.
How is light refracted (bent)?
When light passes from one medium to another.
What is the refractive index?
A measure of how greatly a substance slows the speed of light.
Microbiology: Microscopy
Refractive index
Light is refracted when...
Focal point
Focal length
measure of how greatly a substance slows the velocity of light
it passes through one medium to another
Location where the light is focused
distance between center of lens and the focal point
Refractive index
measure of how greatly a substance slows the velocity of light
Light is refracted when...
it passes through one medium to another
17 terms
Microbiology Microscopy
Types of light microscopes?
Types of electron microscopes?
What does an atomic force microscope d…
What does a bright-field microscope do…
Bright-field, dark-field, phase-contrast, fluorescence, scann…
transmission (TEM), scanning (SEM)
A probe moves across the surface of a sample, producing a map…
It illuminates the field background. Most common type of micr…
Types of light microscopes?
Bright-field, dark-field, phase-contrast, fluorescence, scann…
Types of electron microscopes?
transmission (TEM), scanning (SEM)
49 terms
Microbiology Chapter 3 - Microscopy & Metric Units
resolution
Zaccharias Jenseen
Illuminator
Condenser
Ability to see fine details of a specimen, or the maximum dis…
is credited with making the first compound microscope in 17th…
The light source for the Microscope.
Focuses light through the specimen.
resolution
Ability to see fine details of a specimen, or the maximum dis…
Zaccharias Jenseen
is credited with making the first compound microscope in 17th…
Microbiology Microscopy
Types of light microscopes?
Types of electron microscopes?
What does an atomic force microscope d…
What does a bright-field microscope do…
Bright-field, dark-field, phase-contrast, fluorescence, scann…
transmission (TEM), scanning (SEM)
A probe moves across the surface of a sample, producing a map…
It illuminates the field background. Most common type of micr…
Types of light microscopes?
Bright-field, dark-field, phase-contrast, fluorescence, scann…
Types of electron microscopes?
transmission (TEM), scanning (SEM)
7 terms
Microbiology - Microscopy
Light Microscopy
Brightfield
Phase contrast
Darkfield
Compound light microscope uses visible light to illuminate sp…
Dark objects are visible against a bright background.... Light r…
Increases contrast without stain.... Light reaches specimen from…
Light objects are visible against a dark background.... Light re…
Light Microscopy
Compound light microscope uses visible light to illuminate sp…
Brightfield
Dark objects are visible against a bright background.... Light r…
6 terms
Microbiology - Microscopy
Bright Field
Dark Field
Phase Contrast
Confocal
Aluminting cells
Silhouettes of cells
Looking into detail in a cell
3D imaging
Bright Field
Aluminting cells
Dark Field
Silhouettes of cells
55 terms
Microbiology - Ch 3 Microscopy & Structure and Function
magnification
resolution
resolving power
immersion oil
lenses combine to enlarge objects. is equal to the factor of…
usefulness of microscope depends on its ability to do this wi…
this is defined as the minimum distance existing between two…
this reduces light refraction. light bends as it moves from g…
magnification
lenses combine to enlarge objects. is equal to the factor of…
resolution
usefulness of microscope depends on its ability to do this wi…
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