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Study sets matching "microbiology lab practical"

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Study sets matching "microbiology lab practical"

42 terms
Microbiology Practical
simple stain
negative stain
Gram stain
acid-fast stain
cells are stained with colored dye to make them more visible…
Uses dye solution where chromogen is acidic and carries a neg…
differential stain; purple is positive because has thicker pe…
differential stain to detect the presence of mycolic acid (a…
simple stain
cells are stained with colored dye to make them more visible…
negative stain
Uses dye solution where chromogen is acidic and carries a neg…
152 terms
Microbiology Practical
gram postitive
gram negative
safranin
Crystal violet
cell wall contain large layer of peptidoglycan and no lipopol…
Cell walls containing a thin layer of peptidoglycan and an ou…
Name the dye color
Name the dye color
gram postitive
cell wall contain large layer of peptidoglycan and no lipopol…
gram negative
Cell walls containing a thin layer of peptidoglycan and an ou…
181 terms
Microbiology Practical
ocular lens
body tube
objective lens
stage
remagnifies the image formed by the objective lens
transmits the image from objectives lens to the ocular lens
primary lenses that magnify the specimen
holds the microscope in position
ocular lens
remagnifies the image formed by the objective lens
body tube
transmits the image from objectives lens to the ocular lens
230 terms
microbiology practical
simple stain
capsule stain
gram stain
endospore stain
simple stain
capsule stain
34 terms
Microbiology Practical
Interpret an antibiotic sensitivity test
Interpret an enterotube
Mannitol-Salt agar
Blood agar
detected using antibiotic disks and quantified by zones of in…
compare the rapid test tube with the chart provided and deter…
b) positive - yellow... negative - red... c) tests for the ability…
b) alpha hemolytic - greenish... beta hemolytic - colorless, com…
Interpret an antibiotic sensitivity test
detected using antibiotic disks and quantified by zones of in…
Interpret an enterotube
compare the rapid test tube with the chart provided and deter…
71 terms
Microbiology Practical
What does a gram stain test for?
Is the gram stain a selective or diffe…
List the steps of the gram stain?
What color is a gram positive bacteria…
Gram (-) vs Gram (+)
Differential
1. Apply bacteria from slant or broth to a slide... 2. If from a…
Purple
What does a gram stain test for?
Gram (-) vs Gram (+)
Is the gram stain a selective or diffe…
Differential
21 terms
Microbiology Practical
gram positive
gram negative
rod shaped, single cells
Rod shaped in clusters
gram positive
gram negative
Microbiology Practical
gram postitive
gram negative
safranin
Crystal violet
cell wall contain large layer of peptidoglycan and no lipopol…
Cell walls containing a thin layer of peptidoglycan and an ou…
Name the dye color
Name the dye color
gram postitive
cell wall contain large layer of peptidoglycan and no lipopol…
gram negative
Cell walls containing a thin layer of peptidoglycan and an ou…
117 terms
Microbiology Practical
gram postitive
gram negative
safranin
Crystal violet
cell wall contain large layer of peptidoglycan and no lipopol…
Cell walls containing a thin layer of peptidoglycan and an ou…
Name the dye color
Name the dye color
gram postitive
cell wall contain large layer of peptidoglycan and no lipopol…
gram negative
Cell walls containing a thin layer of peptidoglycan and an ou…
12 terms
Microbiology Practical
Fungi
Fungi
Bacteria
Bacteria
Fungi
Fungi
8 terms
Microbiology Practical
enterobius vermicularis
Taenia pisiformis
Clonorchis sinesis
Plasmodium
enterobius vermicularis
Taenia pisiformis
20 terms
Microbiology Practical
Saccaharomyces Cerevisiae
Candida Albicans
Rhizopus Nigricans
Penicillium
what is this?
What is this?
what is this?
What is this?
Saccaharomyces Cerevisiae
what is this?
Candida Albicans
What is this?
Microbiology Practical
Amoeba Proteus
Spirillum Volutans
E.coli
staphylococcus aureus
protozoa, HARMLESS
spirillum
gram negative, bacilli
gram positive cocci
Amoeba Proteus
protozoa, HARMLESS
Spirillum Volutans
spirillum
85 terms
Microbiology Practical
Magnification
Contrast
Resolution
Wavelength
How much larger a specimen's image appears compared to it's a…
How well the specimen stands out from the bright background
Ability to see details and distinguish structures, clarity of…
distance between 2 adjacent peaks
Magnification
How much larger a specimen's image appears compared to it's a…
Contrast
How well the specimen stands out from the bright background
13 terms
Microbiology Practical
gram positive
gram negative
rod shaped, single cells
Rod shaped in clusters
gram positive
gram negative
20 terms
Microbiology Practical
simple stain
negative stain
Gram stain
acid-fast stain
cells are stained with colored dye to make them more visible…
Uses dye solution where chromogen is acidic and carries a neg…
differential stain; purple is positive because has thicker pe…
differential stain to detect the presence of mycolic acid (a…
simple stain
cells are stained with colored dye to make them more visible…
negative stain
Uses dye solution where chromogen is acidic and carries a neg…
42 terms
Microbiology Practical
simple stain
negative stain
Gram stain
acid-fast stain
cells are stained with colored dye to make them more visible…
Uses dye solution where chromogen is acidic and carries a neg…
differential stain; purple is positive because has thicker pe…
differential stain to detect the presence of mycolic acid (a…
simple stain
cells are stained with colored dye to make them more visible…
negative stain
Uses dye solution where chromogen is acidic and carries a neg…
55 terms
microbiology practical
compound light
x100
stage, base, ocular lens, body, arm, i…
bacillus, coccus, spiral
1)What type of microscope is this?
2)The microscope is set up on which magnification?
3-9) Name they parts of the microscope
10-12) name the shapes of bacteria
compound light
1)What type of microscope is this?
x100
2)The microscope is set up on which magnification?
128 terms
Microbiology Practical
gram postitive
gram negative
safranin
Crystal violet
cell wall contain large layer of peptidoglycan and no lipopol…
Cell walls containing a thin layer of peptidoglycan and an ou…
Name the dye color
Name the dye color
gram postitive
cell wall contain large layer of peptidoglycan and no lipopol…
gram negative
Cell walls containing a thin layer of peptidoglycan and an ou…
108 terms
Microbiology lab practical I- review
Disposal of petri plate
Disposal of test tubes
Transient bacteria
resident bacteria
bio-hazardous waste
return to designated cart with NO LABELS
bacteria transferred from other people or things
bacteria that you carry all the time (normal flora)
Disposal of petri plate
bio-hazardous waste
Disposal of test tubes
return to designated cart with NO LABELS
6 terms
Microbiology Practical
simple stain
Gram stain
mannitol salts agar
eosin methylene blue agar (EMB)
cells are stained with colored dye to make them more visible…
differential stain; purple is positive because has thicker pe…
mannitol provides substrate for fermentation and makes medium…
undefined, selective, differential medium; twofold purpose: 1…
simple stain
cells are stained with colored dye to make them more visible…
Gram stain
differential stain; purple is positive because has thicker pe…
51 terms
Microbiology Practical
Wet Mount
Simple streak
Quadrant streak
What information needs to be marked on…
Normal preparation of slides
regular streak on an agar plate
Streak 4 times to isolate colonies
Name, Date, Inoculate organism
Wet Mount
Normal preparation of slides
Simple streak
regular streak on an agar plate
152 terms
Microbiology Practical
gram postitive
gram negative
safranin
Crystal violet
cell wall contain large layer of peptidoglycan and no lipopol…
Cell walls containing a thin layer of peptidoglycan and an ou…
Name the dye color
Name the dye color
gram postitive
cell wall contain large layer of peptidoglycan and no lipopol…
gram negative
Cell walls containing a thin layer of peptidoglycan and an ou…
152 terms
Microbiology Practical
gram postitive
gram negative
safranin
Crystal violet
cell wall contain large layer of peptidoglycan and no lipopol…
Cell walls containing a thin layer of peptidoglycan and an ou…
Name the dye color
Name the dye color
gram postitive
cell wall contain large layer of peptidoglycan and no lipopol…
gram negative
Cell walls containing a thin layer of peptidoglycan and an ou…
17 terms
microbiology practical
oscillatoria
spirogyra
volvox
diatoms
oscillatoria
spirogyra
37 terms
Microbiology Practical
BSA original color
BSA
What color is salmonella on BSA
BGA
dull green
Bismuth sulfide Agar, contains ferrous sulfite to indicate H2…
dark brown, black
Brilliant Green Agar, not green, red. Contains phenol red to…
BSA original color
dull green
BSA
Bismuth sulfide Agar, contains ferrous sulfite to indicate H2…
105 terms
Microbiology Practical
What are the two types of lenses on a…
Labeled Microscope
scanning
low power
eyepiece, or ocular... &... Objective
4X
10X
What are the two types of lenses on a…
eyepiece, or ocular... &... Objective
Labeled Microscope
108 terms
Microbiology Lab Practical
Bacillus
Bacillus
Staphylococcus
Staphylococcus
Bacillus
Bacillus
19 terms
Microbiology Practical
condenser
iris diaphragm
ocular lenses
objective lenses
several lenses that concentrate light on the slide by focusin…
controls the angle and size of the cone of light
eyepiece... 10x magnification
revolving nose-piece consists of the 4 different lens
condenser
several lenses that concentrate light on the slide by focusin…
iris diaphragm
controls the angle and size of the cone of light
158 terms
Microbiology Practical
Prokaryote
Eukaryote
Domain Eukarya
Fungi
-One circular chrmosome, not in a membrane... - No histones... - No…
-Paired chromosome in a nuclear membrane... - Histones... - Organel…
Fungi... Anamalia... Protista... Plantae
Unicellular (yeasts) or Multicellular (molds and mushrooms)... C…
Prokaryote
-One circular chrmosome, not in a membrane... - No histones... - No…
Eukaryote
-Paired chromosome in a nuclear membrane... - Histones... - Organel…
208 terms
Lab Practical: Microbiology
hyphae
mycelium
eosinophil
neutrophil function
filaments of an ascospore
mat of hyphae in an ascospore
part of innate immunity
phagocytosis
hyphae
filaments of an ascospore
mycelium
mat of hyphae in an ascospore
34 terms
Medical Microbiology Practical 3
Balantidium coli
Plasmodium malariae
Amoeba proteus
Leischmania donovani
Balantidium coli
Plasmodium malariae
102 terms
Microbiology Practical
What are the two types of lenses on a…
Labeled Microscope
scanning
low power
eyepiece, or ocular... &... Objective
4X
10X
What are the two types of lenses on a…
eyepiece, or ocular... &... Objective
Labeled Microscope
58 terms
Microbiology Practical
pure culture
quadrant streak
pour plate method
how must you store plates?
pure colonies that arise from one bacteria and represent one…
take a loop full of bacteria and drag through four quadrants…
take a small amount of culture and add it to liquid agar, poo…
upside down to prevent condensation, always label on the base
pure culture
pure colonies that arise from one bacteria and represent one…
quadrant streak
take a loop full of bacteria and drag through four quadrants…
29 terms
Microbiology Practical
Negative Stain
Gram Stain
Acid Fast Stain
Spore Stain
• Primary Stain: Nigrosin
HEAT FIXED... 1 Color ... (+) Purple... (-) Pink... Primary Stain: Crys…
2 Colors... thick fast, waxy cell wall... Primary: Carbol Fuschin…
HEAT FIXED... (+) Green... (-) Red... Primary: Malachite Green ... Count…
Negative Stain
• Primary Stain: Nigrosin
Gram Stain
HEAT FIXED... 1 Color ... (+) Purple... (-) Pink... Primary Stain: Crys…
55 terms
Microbiology Lab Practical - Final
Inoculating Loop
Inoculating Needle
Bacillus and Clostridium
Flame the loop
What is this tool we used in lab?
What is this tool we used in lab with the pointed end?
Which two types of Bacteria produce endospores?
What do you for aseptic technique whenever you use the inocul…
Inoculating Loop
What is this tool we used in lab?
Inoculating Needle
What is this tool we used in lab with the pointed end?
97 terms
Microbiology Lab Practical #2
Sugar Fermentation
Lactose broth contains
Phenol Red Indicator in Sugar Fermenta…
Results of Sugar Fermentation
- Test for bacteria's ability to ferment the sugars lactose,…
- Beef extract... - Gelatin peptone... - Sugar
- Added to indicate acid production from fermentation
- Positive results are yellow after the indicator has been ad…
Sugar Fermentation
- Test for bacteria's ability to ferment the sugars lactose,…
Lactose broth contains
- Beef extract... - Gelatin peptone... - Sugar
38 terms
Microbiology practical
simple stain
negative stain
acid-fast stain
How do you dry slides?
-Cells are negative and basic (pH is high) ... -simple stains ha…
- you use a stain that is negative and will be repelled by th…
- done to organisms with mycolic acid (waxy-substance in cell…
-with bibulous paper
simple stain
-Cells are negative and basic (pH is high) ... -simple stains ha…
negative stain
- you use a stain that is negative and will be repelled by th…
48 terms
Microbiology practical
Primary media
What is the purpose of primary media
Enriched media
Sheep Blood Agar (SBA)
first media used to culture a specimen
1) cultural all bacterial species present to determine which…
contain nutrients (blood, or amino acids) to support the grow…
distinguishes certain bacteria
Primary media
first media used to culture a specimen
What is the purpose of primary media
1) cultural all bacterial species present to determine which…
60 terms
Microbiology practical 2
micro titer plate
transfer pipette
glass beaker
microcentrifuge tube
micro titer plate
transfer pipette
16 terms
Spelling practice for Microbiology practical
Vibrio cholerae
Candida albicans
Trichomonas vaginalis
Streptococcus mutans
Cholera
Candidiasis
Parasitic STD
Oral flora
Vibrio cholerae
Cholera
Candida albicans
Candidiasis
150 terms
Microbiology Practical
Safety and Lab Practice... If you spill…
Safety and Lab Practice... immediately…
Safety and Lab Practice... report cuts…
Safety and Lab Practice... report broke…
stop and yell "spill" and get the instructor, the instructor…
warm water and soap
to the instructor immediately
disposed of properly and not in regular trash cans
Safety and Lab Practice... If you spill…
stop and yell "spill" and get the instructor, the instructor…
Safety and Lab Practice... immediately…
warm water and soap
26 terms
Microbiology Practical
MacConkey Agar
Eosin Methylene Blue Agar
Phenyl Ethlyn Alcohol Agar
Mannitol Salt
Selects for gram-negative bacteria
gram-negative bacteria
Most gram-positive cocci
staphylococci and bacteria that can withstand high salt conce…
MacConkey Agar
Selects for gram-negative bacteria
Eosin Methylene Blue Agar
gram-negative bacteria
48 terms
Microbiology Lab Practical
How do you find total magnification?
What is this piece called on the micro…
What is the function of the ocular?
What is this piece called on the micro…
Multiply the power of the ocular (10x) by the power of the sc…
ocular (eye piece)
magnifies the image tenfold----binocular (two lenses)
objective lens
How do you find total magnification?
Multiply the power of the ocular (10x) by the power of the sc…
What is this piece called on the micro…
ocular (eye piece)
101 terms
Microbiology Practical
Eyepiece
Diopter Ring
Objective Lenses
Nosepiece
where your eyes look through
this is what you adjust on one of your eyepieces to account f…
series of lenses located on the nosepiece that further increa…
holds the objective lenses, rotates to allow you to change be…
Eyepiece
where your eyes look through
Diopter Ring
this is what you adjust on one of your eyepieces to account f…
91 terms
Microbiology Lab Final Practical
pure culture
1. prevent slide/lens from breaking... 2.…
colonial morphology
cell morphology
where you have successfully isolated one species of bacteria…
Why is using focal length important when using oil immersion…
ex. circular, irregular, filamentous, punctiform, elevation
ex. bacilla, cocci, spirilla/spirochette, pleomorphic
pure culture
where you have successfully isolated one species of bacteria…
1. prevent slide/lens from breaking... 2.…
Why is using focal length important when using oil immersion…
77 terms
Microbiology Practical
Condenser lens
Refraction
Objective lens
Real image
Concentrates the light and makes illumination of the specimen…
Bending of light as it passes from a medium with one refracti…
The microscope lens that first produces magnification of the…
Magnified image of a specimen produced by the objective lens…
Condenser lens
Concentrates the light and makes illumination of the specimen…
Refraction
Bending of light as it passes from a medium with one refracti…
82 terms
Microbiology Lab - Final Practical
Plasmodium; Ring Stage
Plasmodium Schizont Stage; Malaria
Entamoeba; Trophozoite
Entamoeba; Cyst
What are the arrows pointing to?
What is "A" pointing to? What disease is this organism respon…
Name this organism; What stage is it in?
What is this organism? What phase is it in?
Plasmodium; Ring Stage
What are the arrows pointing to?
Plasmodium Schizont Stage; Malaria
What is "A" pointing to? What disease is this organism respon…
21 terms
Microbiology Practical
Turns green metallic on Eosin Methylen…
Gram positive cocci, ferments mannitol…
Gram positive cocci, does not ferment…
Gram negative bacillus, non-lactose fe…
Escherichia coli
Staphylococcus aureus
Staphylococcus epidermidis
Proteus vulgaris
Turns green metallic on Eosin Methylen…
Escherichia coli
Gram positive cocci, ferments mannitol…
Staphylococcus aureus
44 terms
Microbiology Practical
Aseptic technique
Simple stain
Gram stain
Catalase test
Transferring bacteria without contamination
Discerning shape and arrangement of bacteria
Identifying the presence or absence of a thick layer of pepti…
Distinguishing between the genus staphylococcus and streptoco…
Aseptic technique
Transferring bacteria without contamination
Simple stain
Discerning shape and arrangement of bacteria
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