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Study sets matching "microbiology questions"

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Study sets matching "microbiology questions"

19 termscreated on December 4, 2016
Big mixed culture unknown
Media used in big unknown
fermentation of a sugar vs. oxidation…
Why were different sugars used>
Can you predict the fermentation of a…
1. Blood Agar Plate (BAP) typically allows both organisms to…
-Fermentation: fermentation is the anaerobic metabolism of a…
-because different microbes have different genes to allow for…
No, the bacteria needs to have genes to ferment different sug…
Media used in big unknown
1. Blood Agar Plate (BAP) typically allows both organisms to…
fermentation of a sugar vs. oxidation…
-Fermentation: fermentation is the anaerobic metabolism of a…
16 termscreated on December 3, 2016
Microbiology Lab Quiz 8: Protozoans & Fungi
Aspergillus
Penicillium
Rhizopus
Saccharomyces
Fungi: lung infection
Fungi
Fungi: lung infection
Fungi:
Aspergillus
Fungi: lung infection
Penicillium
Fungi
22 termscreated on December 3, 2016
Microbiology 2164 Neubauer Lab Quiz Dental caries & throat swab
What is Snyder Agar used for?
Why is Snyder Agar used for testing su…
What is pH of S.Agar?
What fermentable carb is added to S.Ag…
Measures susceptibility to Dental caries
The medium is formulated to favor growth of oral bacteria and…
4.8
Glucose
What is Snyder Agar used for?
Measures susceptibility to Dental caries
Why is Snyder Agar used for testing su…
The medium is formulated to favor growth of oral bacteria and…
63 termscreated on December 3, 2016
Microbiology 2164 Neubauer Lab on Parisitology
Subphylum of Plasmondium falcipatum
Sporozoa are motile or non-motile?
Plasmondium falcipatum
What disease does Plasmondium falcipat…
Sporozoa
non-motile
malaria
Subphylum of Plasmondium falcipatum
Sporozoa
Sporozoa are motile or non-motile?
non-motile
39 termscreated on December 3, 2016
Microbiology Exam 3: chapter 14
symbiont
Parasitism
Commensalism
Mutualism
has developed a long-standing relationship w/ another organis…
one species benefits, the other is harmed
One species benefits the other is unaffected
Both species benefit
symbiont
has developed a long-standing relationship w/ another organis…
Parasitism
one species benefits, the other is harmed
145 termscreated on December 3, 2016
Microbiology Lect - FINAL
Microbiology
Microorganisms
Eukaryotic
Prokaryotic
The study of organisms too small to be seen without a microsc…
Bacteria, Fungi, Viruses, Prions, Viroids
Multicellular organisms that have membrane-enclosed organelle…
Unicellular organisms that do not have membrane-bound organel…
Microbiology
The study of organisms too small to be seen without a microsc…
Microorganisms
Bacteria, Fungi, Viruses, Prions, Viroids
20 termscreated on December 3, 2016
Microbiology
Coccus
Bacillus
What is Gram staining used for?
Why do we heat fix our sample to the s…
spherical cells
shaped like rods or cylinders
To categorize bacteria as gram positive or gram negative
To prevent the sample from washing off, preserve cell morphol…
Coccus
spherical cells
Bacillus
shaped like rods or cylinders
84 termscreated on December 3, 2016
Final: Unique Bacteria
Bacteria taxonomy based on sequencing…
Most info about bacteria that we know…
What 3 bacteria lineages are the oldes…
Hyperthermophilic bacteria
16S rRNA
Proteobacteria
Aquifex... Thermodesulfobacterium Thermotoga
Aquifex... Thermodesulfobacterium... Thermotoga
Bacteria taxonomy based on sequencing…
16S rRNA
Most info about bacteria that we know…
Proteobacteria
78 termscreated on December 3, 2016
Microbiology Final Exam Review (Chapters 10, 11, and 12)
Mutualism
Commensalism
Neutralism
Parasitism
A symbiotic relationship is one that is beneficial to both pa…
A symbiotic relationship is one that is beneficial to one mem…
A symbiotic relationship is one where neither member benefits…
A symbiotic relationship in which one member benefits to the…
Mutualism
A symbiotic relationship is one that is beneficial to both pa…
Commensalism
A symbiotic relationship is one that is beneficial to one mem…
141 termscreated on December 3, 2016
Final: Part I review: History, microscopy, cell walls, cytoplasmic components, morphology and envelopes
Objective lens
Focal length
Relationship between focal length and…
Power/magnification
most important part of a microscope; used to focus light from…
difference between optic lens and focal point; thicker lens i…
P = 1/f... P=power/magnification... f=focal point... Shorter focal le…
affected by focal length. affected by thickness of lens. thin…
Objective lens
most important part of a microscope; used to focus light from…
Focal length
difference between optic lens and focal point; thicker lens i…
277 termscreated on December 3, 2016
Mid-Term Reveiw
anatomy
anthropology
bacteriology
biochemistry
study of body structures
study of the history of man
study of bateria
study of chemical compounds and reactions present in loving t…
anatomy
study of body structures
anthropology
study of the history of man
87 termscreated on December 3, 2016
Biology Lab Test
Opposite (leaf arrangement)
Alternate (leaf arrangement)
Simple (leaf blade)
Once pinnately compound (leaf blade)
Opposite (leaf arrangement)
Alternate (leaf arrangement)
11 termscreated on December 3, 2016
Cutaneous Microbiology and Immunology
Normal skin flora
Microbial inhabitants of the skin
Keratinocytes as innate immune players
S. epidermidis educates CD8 T-cells
Gram (+) in dry areas ... Gram (-) in wet areas... Most organisms…
Staph epidermidis (main aerobic)... S. aureus in nose, perineum…
Toll-like receptors (TLRs)... Cytokines and chemokines... - TNF-al…
Dendritic cells sample S. epidermidis antigens... Presentation…
Normal skin flora
Gram (+) in dry areas ... Gram (-) in wet areas... Most organisms…
Microbial inhabitants of the skin
Staph epidermidis (main aerobic)... S. aureus in nose, perineum…
46 termscreated on December 3, 2016
Microbiology Lab Final
1000
.001
gram positive
gram negative
1 mL = _____ µl
1 µl = _____ mL
bacteria turn purple due to thick peptidoglycan layer in cell…
bacteria are pink - lack peptidoglycan wall + don't retain co…
1000
1 mL = _____ µl
.001
1 µl = _____ mL
9 termscreated on December 3, 2016
Lab 23
1. Used to specifically grow gram posi…
1. Contain mayn polycationic regions t…
Inhibits DNA gyrase topoisonmerase ... -…
This is Columbia CNA with 5% sheeps bl…
1. What is Columbia CNA with 5% sheep blood agar used for?... 2…
How does the Colistin antibiotic work?
How does Nalidixic antibiotic work
1. Used to specifically grow gram posi…
1. What is Columbia CNA with 5% sheep blood agar used for?... 2…
1. Contain mayn polycationic regions t…
How does the Colistin antibiotic work?
43 termscreated on December 3, 2016
microbiology lab final - slides
aspergilus conidiophores, found in soi…
trichinella spirals, trichonosis, dise…
giardia cyst. transmission is by conta…
giardia sps;trophotoite. transmission…
aspergilus conidiophores, found in soi…
trichinella spirals, trichonosis, dise…
24 termscreated on December 3, 2016
Cutaneous Microbiology & Immunology
A
B
C
D
_______________ is the main aerobic bacterial resident of the…
________________ colonizes normal skin in the nose, perineum…
________________ (a group of bacteria including Propionibacte…
__________________ (Group A: S. pyogenes and Group B: S. agal…
A
_______________ is the main aerobic bacterial resident of the…
B
________________ colonizes normal skin in the nose, perineum…
46 termscreated on December 3, 2016
Medical Microbiology Ch. 17
Innate Immunity
Immunity
Antigen (Ag)
Antibody (Ab)
Nonspecific* Defenses against any pathogen
Specific antibody & lymphocyte response to an antigen
A substance that causes the body to produce specific antibodi…
Proteins made in response to an antigen and that can combine…
Innate Immunity
Nonspecific* Defenses against any pathogen
Immunity
Specific antibody & lymphocyte response to an antigen
28 termscreated on December 3, 2016
Lab 22
- Contains one or more specific compou…
1. Contains one or more specific compo…
1. A simple way to screen out certain…
1. Bacteria that reside in the gut of…
1. What is a selective media?... 2. What does it contain?... 3. Wha…
1. What is a differential media?... 2. What are the components?
1. Why are selective and differential media an extremely usef…
What are Coliforms
- Contains one or more specific compou…
1. What is a selective media?... 2. What does it contain?... 3. Wha…
1. Contains one or more specific compo…
1. What is a differential media?... 2. What are the components?
94 terms
created on December 3, 2016
Oral Microbial Flora
200,000 years
6 million years
sepsis
65 million years ago
how long have homo sapiens been around?
how long have human ancestors been around?
dinosars had very bad halitosis which would lead to what afte…
dinosaurs were around how long ago?
200,000 years
how long have homo sapiens been around?
6 million years
how long have human ancestors been around?
48 termscreated on December 3, 2016
Microbiology 3
Capsid
Capsomere
Enveloped
Helical Virus
(2)... Outer protein coat of a virus
A subunit of the protein capsid of a virus.
Enclosed completely with a membrane
Nucleic acid is helical, capsid is helical around it
Capsid
(2)... Outer protein coat of a virus
Capsomere
A subunit of the protein capsid of a virus.
49 termscreated on December 3, 2016
Micro Lab Final BIO 210
Sugar fermentation test: Name the medi…
Mixed acid fermentation test: What is…
Butanediol fermentation: AKA? Medium?…
Catalase production: Medium? Enzyme? C…
Medium- Sugar fermentose broth ... Control- Escherichia coli... Pos…
AKA- methyl red. Medium- MR-VP media. Enzyme- Formic hydrogen…
AKA- vages proskauer. Medium- MR-VP. Control- Enterobacter ae…
Medium- hyrdogen peroxide. Enzyme- catalase. M/O- Staphylococ…
Sugar fermentation test: Name the medi…
Medium- Sugar fermentose broth ... Control- Escherichia coli... Pos…
Mixed acid fermentation test: What is…
AKA- methyl red. Medium- MR-VP media. Enzyme- Formic hydrogen…
26 termscreated on December 3, 2016
Toxic Syndromes
superantigen
postpartum & surgical wound infections…
Staphylococcal toxic shock syndrome
CA-MRSA
this class of molecule binds directly to MHC II of antigen-pr…
What are some non-menstrual related causes of Toxic Shock Syn…
This condition is associated with an excessive INF-g producti…
what staphylococcal spp. is most likely to cause TSS?
superantigen
this class of molecule binds directly to MHC II of antigen-pr…
postpartum & surgical wound infections…
What are some non-menstrual related causes of Toxic Shock Syn…
30 termscreated on December 3, 2016
Chapter 8
gyrase or topoisomerase
error rate (fidelity) of DNAp
exons
introns
relaxes supercoiling ahead of rep. fork.
1/billion
code for protein.
don't code for protein.
gyrase or topoisomerase
relaxes supercoiling ahead of rep. fork.
error rate (fidelity) of DNAp
1/billion
37 termscreated on December 3, 2016
Chapter 5 Terms
Holoenzyme
B1
B2
Niacin
protein (apoenzyme) + cofactor (vitamins)
Thiamine, part of coenzyme cocarboxylase. Many functions, lik…
Riboflavin, coenzyme in flavoproteins, active in electron tra…
Part of NAD, active in e- transfers.
Holoenzyme
protein (apoenzyme) + cofactor (vitamins)
B1
Thiamine, part of coenzyme cocarboxylase. Many functions, lik…
11 termscreated on December 3, 2016
Chapter 2 Terms
Sucrose
Lactose
Maltose
Dextran
glucose + fructose
galactose + glucose
glucose + glucose
polyS "slime coat" of some bac., in saline for easier infusion.
Sucrose
glucose + fructose
Lactose
galactose + glucose
108 termscreated on December 3, 2016
Microbiology Chapter 17
compare/contrast antibody mediated imm…
Epitope
Antigens
Antibodies are the immune facilitators
Antibody Mediated : extra cellular (blood and lymph), MHC II,…
region on an antigen to where an antibody binds
foreign substances that evoke immune response (aka immunogens…
What are the immune facilitators for adaptive immunity?
compare/contrast antibody mediated imm…
Antibody Mediated : extra cellular (blood and lymph), MHC II,…
Epitope
region on an antigen to where an antibody binds
96 termscreated on December 3, 2016
Microbiology Lab Practicuum
isolation technique
Streak plate
Colony-forming unit (CFU)
Steps for Streaking Plate with Loop
method to obtain a pure culture (single species) from a mixed…
isolation technique in which a bacterial sample is streaked o…
colony origin (e.g. individual cells, pairs, chains, clusters…
1. Use sterile loop to obtain a mixed culture... 2. Open agar pl…
isolation technique
method to obtain a pure culture (single species) from a mixed…
Streak plate
isolation technique in which a bacterial sample is streaked o…
93 termscreated on December 3, 2016
Microbiology Chapter 17: Adaptive Immunity
adaptive immunity
how was adaptive immunity first observ…
immunization
when does adaptive immunity come into…
range of microbial defenses that target specific pathogens, w…
when a person recovered from small pox or measles that person…
mimicking adaptive immunity by deliberately exposing people t…
when innate defenses fail to stop a microbe
adaptive immunity
range of microbial defenses that target specific pathogens, w…
how was adaptive immunity first observ…
when a person recovered from small pox or measles that person…
62 termscreated on December 3, 2016
Micro Bell Ringer Prep
Occupational Safety and Health Adminis…
- Gloves... - Goggles... - Mask
Magnification of an object is obtained…
Oculars or Eyepieces
What does OSHA stand for?
List 3 pieces of proper PPE.
How does magnification occur with a Light Microscope?
What are these called?
Occupational Safety and Health Adminis…
What does OSHA stand for?
- Gloves... - Goggles... - Mask
List 3 pieces of proper PPE.
24 termscreated on December 3, 2016
Y1S1-Block 6-Microbiology-Sporozoans (unfinished)
How do sporozoans move?
How do Sporozoans reproduce?
What is the term for the asexual perio…
What is the term for the sexually repr…
have characteristics of amoeba and flagellates so:... -pseudopo…
-asexually... and... -sexually
Schizogony
Sporogony/Gametogony
How do sporozoans move?
have characteristics of amoeba and flagellates so:... -pseudopo…
How do Sporozoans reproduce?
-asexually... and... -sexually
48 termscreated on December 3, 2016
Microbiology Lab Practical Test 2
Helminths
Ascaris
double pic of Ascaris
Enterobius (pinworm)
multicellular worms: roundworms and flatworms... ascaris, entero…
intestinal roundworm parasite... disease: ascaris lumbricoides... t…
Another picture...... female: wider, straight tail ... male: thinne…
pinworm; intestinal roundworm parasite... treated with Mebendazo…
Helminths
multicellular worms: roundworms and flatworms... ascaris, entero…
Ascaris
intestinal roundworm parasite... disease: ascaris lumbricoides... t…
12 termscreated on December 3, 2016
Microbiology Biochemical Tests and Media
Indole Test
VP Test
Methyl Red Test
Fermentation Broths
Tests the ability of bacteria to degrade the amino acid, tryp…
Tests for fermentation of glucose and the production of aceto…
Tests for mixed acid fermentation of glucose which produced l…
Tests the bacteria's ability to ferment different sugars (glu…
Indole Test
Tests the ability of bacteria to degrade the amino acid, tryp…
VP Test
Tests for fermentation of glucose and the production of aceto…
31 termscreated on December 3, 2016
Exam 3 Lec 16
Human Microbiome
The human body
Hypothesis: We are part of a
Molecular Phylogenetic
The microbial cells, their genes, and the sites they inhabit.…
is a combination of 10^13-10^14 eukaryotic cells (our cells)…
supraorganism ... = microorganism cells and genes provide toxins…
We commonly use the polymerase chain reaction to amplify rRNA…
Human Microbiome
The microbial cells, their genes, and the sites they inhabit.…
The human body
is a combination of 10^13-10^14 eukaryotic cells (our cells)…
53 termscreated on December 3, 2016
Y1S1-Block 6-Microbiology-Protozoans (Exlcuding Sporozoans)
Protozoans are _____________ celled eu…
How are protozoans classified?
How do amoebae move and how do they re…
How do ciliates move and how do they r…
single
based on how they move
-pseudopodia... -asexually
-cilia... -asexually
Protozoans are _____________ celled eu…
single
How are protozoans classified?
based on how they move
136 termscreated on December 3, 2016
Gram - Bacteria
all aerobic gram negative nonenteric b…
aka lipid A... this is released upon the…
What does endotoxin release trigger? H…
Pseudomonas and Burkholderia are both…
endotoxin
endotoxin
release of cytokines by macrophages ... with the addition of abx…
opportunistic
all aerobic gram negative nonenteric b…
endotoxin
aka lipid A... this is released upon the…
endotoxin
25 termscreated on December 3, 2016
Bacteria, Fungi, Viruses and more
microbiology
Viruses, bacteria, fungi and parasites
Viruses
How do Viruses spread
study of microscopic organisms including viruses, bacteria, p…
infect the human body and interfere with normal body functions.
non-living particles composed of a nucleic acid (DNA or RNA)…
transmitted through contact, insect bites, and water.
microbiology
study of microscopic organisms including viruses, bacteria, p…
Viruses, bacteria, fungi and parasites
infect the human body and interfere with normal body functions.
98 termscreated on December 3, 2016
Micro Final
What is the study of microbiology?
What are the 6 major groups of organis…
What are the 6 criteria of science
What is an Independent variable?
The study of organisms too small to be seen on a microscope
Bacteria, virus, helminths, protozoa, fungi, algea
Consistent, Observable, Natural, Predictable, Testable, Tenta…
researcher controls; changed by scientist
What is the study of microbiology?
The study of organisms too small to be seen on a microscope
What are the 6 major groups of organis…
Bacteria, virus, helminths, protozoa, fungi, algea
22 termscreated on December 3, 2016
Microbiology
Coccus
Bacillus
What color do gram positive bacteria s…
What color do gram negative bacteria s…
spherical cells
shaped like rods or cylinders
Purple
Red
Coccus
spherical cells
Bacillus
shaped like rods or cylinders
70 termscreated on December 3, 2016
Micro. Lab Final Review
1. Demonstrate correct aseptic techniq…
2. Use the streak plate method to sepa…
3.Use a compound light microscope.
4. Know the names and functions of the…
Procedures used in handing cultures, media, and equipment so…
1. make sure to mark grid on plate A, B, C, D on back of agar…
always start with the 4x or 10x.... then use oil immersion.
4x: red, scanning obj. ... 10x: yellow, lower power obj.... 40x: bl…
1. Demonstrate correct aseptic techniq…
Procedures used in handing cultures, media, and equipment so…
2. Use the streak plate method to sepa…
1. make sure to mark grid on plate A, B, C, D on back of agar…
34 termscreated on December 3, 2016
Final exam terminology
mRNA
rRNA
tRNA
UAA, UAG, UGA
Type of RNA- Encodes protein. Around 1000 bp in length
Type of RNA- Synthesizes protein as part of ribosome
Type of RNA- Shuttles amino acids to ribosome
Three stop codons are?
mRNA
Type of RNA- Encodes protein. Around 1000 bp in length
rRNA
Type of RNA- Synthesizes protein as part of ribosome
25 termscreated on December 3, 2016
Microbiology Lab Practical
Gram Stain
Gram Positive
Gram Negative
Sterile Technique
Inoculate slide... Let plate dry... Pass plate over flame... 2 drops o…
Purple color... Thick cell wall
Red color... Thin membrane
Heat loop till red hot... Cool loop for 10 seconds... Dip loop into…
Gram Stain
Inoculate slide... Let plate dry... Pass plate over flame... 2 drops o…
Gram Positive
Purple color... Thick cell wall
22 termscreated on December 3, 2016
Microbiology lab
Entamoeba histolytica trophozoites... (1…
Mature Entamoeba histolytica cyst... (4 n…
Giardia lamblia trophozoite (cause gia…
Giardia lamblia cyst (4 nuclei)
cause liver abscess
cause intestinal abscess
Entamoeba histolytica trophozoites... (1…
cause liver abscess
Mature Entamoeba histolytica cyst... (4 n…
cause intestinal abscess
75 termscreated on December 3, 2016
Microbiology Lab Final
simple microscopes
simple microscope components
brightfield compound microscope
basic components of a microscope
early microscopes other name
biconvex lens (essentially magnifying glasses)
microscope used in lab and in most labs, dark objects in a br…
base, stage, body tube, and an arm
simple microscopes
early microscopes other name
simple microscope components
biconvex lens (essentially magnifying glasses)
25 termscreated on December 2, 2016
Microbiology Lab Final
Of what value is a simple stain?
smear
fix
chemically fix
used to determine cell morphology, size, and arrangement
a thin film of bacterial cells
- a process that kills bacteria; coagulated proteins from the…
cover the smear with 95% methanol for 1 minute
Of what value is a simple stain?
used to determine cell morphology, size, and arrangement
smear
a thin film of bacterial cells
52 termscreated on December 2, 2016
Lab Practical
Compound Microscope
Light Compound Image
SEM Image
TEM Image
...
Compound Microscope
...
Light Compound Image
20 termscreated on December 2, 2016
Chapter 19 - Allergy & other immune disorders
Immune disorders
Allergy
Histamine
Leukotrienes
most involve adaptive immunity, Humoral (B-cells, antibody) o…
They are caused by inappropriate humoral response to foreign…
"mediator" compound release in response to an allergy. it inc…
"mediator" compound released in response to an allergy. It ca…
Immune disorders
most involve adaptive immunity, Humoral (B-cells, antibody) o…
Allergy
They are caused by inappropriate humoral response to foreign…
56 termscreated on December 2, 2016
Microbiology 101 Lab Final WSU
Microscope
What is a microorganism?
What are the objectives on the microsc…
Which objective requires the use of im…
know... 1. Stage ... 2. Light source ... 3. Arm ... 4.Base ... 5.Condense
Living organism that you need magnification in order to see i…
10X. 40X, 100X
100X because ... 1.Helps with the resolving power... 2.Offsets refr…
Microscope
know... 1. Stage ... 2. Light source ... 3. Arm ... 4.Base ... 5.Condense
What is a microorganism?
Living organism that you need magnification in order to see i…
8 termscreated on December 2, 2016
microbiology lab practical final
Bacillus anthracis
Escherichia coli
Staphylococcus aureus
Bacterial capsule
Bacillus anthracis
Escherichia coli
28 termscreated on December 2, 2016
Microbiology Test #3 (Final)
Exotoxins
Endotoxins
mucinase
hyaluronidase
- Most are proteins... - Gram neg and Gram pos... - Made and releas…
- Only Gram neg... - Released upon bacterial lysis... - Heat resist…
Enzyme that digests the protective coating on mucous membrane…
Enzyme that digests hyaluronic acid
Exotoxins
- Most are proteins... - Gram neg and Gram pos... - Made and releas…
Endotoxins
- Only Gram neg... - Released upon bacterial lysis... - Heat resist…
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