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Study sets matching "spore stain"

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Study sets matching "spore stain"

13 terms
Spore stain
Spore cycle
Vegetative cell
Sporulation
Free spore
1. Vegetative cell... 2. Sporulation... 3. Free spore... 4. Germination
Actively living
dna is copied and placed inside indospore
veg cell dies, made of dipicolinic acid
Spore cycle
1. Vegetative cell... 2. Sporulation... 3. Free spore... 4. Germination
Vegetative cell
Actively living
12 terms
Spore Stain
Spore Stain = Differential Stain
Principle Spore Formers
Spore Stain also called?
Genus Bacillus (aerobic)
2 dyes used
Genera Bacillus and Clostridium
Schaeffer-Fulton Method/Dorner
B. anthracis
Spore Stain = Differential Stain
2 dyes used
Principle Spore Formers
Genera Bacillus and Clostridium
11 terms
spore stain
purpose
what does the malachite green stain st…
decolorizer removes the malachite gree…
decolorizer does not remove malachite…
differentiate between cell wall and spore
cell wall AND spore
cell wall
the spore
purpose
differentiate between cell wall and spore
what does the malachite green stain st…
cell wall AND spore
10 terms
spore stain
endosporses formed when...
endospores are...
in order to kill endospores...
organisms used
there is a lack of nutrients
impermeable to a lot of chemicals and disinfectants (great re…
you must steam under pressure --> 121 deg C
Bacillus cereus
endosporses formed when...
there is a lack of nutrients
endospores are...
impermeable to a lot of chemicals and disinfectants (great re…
Spore staining
malachite green
malachite steam
water
safranin
primary stain
mordant
decolorizer
counterstain
malachite green
primary stain
malachite steam
mordant
6 terms
Spore stain
What type of stain is Spore? Function?
Primary stain?
Counterstaining with
Why do we need steam?
Differential; detects endospores formed by Bacillus and Clost…
Malachite green (over steam for 5 min)
Safranin O (20-30s)
Stains more easily penetrate proteins
What type of stain is Spore? Function?
Differential; detects endospores formed by Bacillus and Clost…
Primary stain?
Malachite green (over steam for 5 min)
16 terms
The Spore Stain
Explain the term vegetative as it appl…
Why are spores more difficult to stain…
Why are spores refractile?
Explain the terms sporulation and germ…
active, living metabolized cells
spores have an extremely thick spore coat
Because it has many layers,, light will bounce off of it
sporulation: the production of the endospore within the veget…
Explain the term vegetative as it appl…
active, living metabolized cells
Why are spores more difficult to stain…
spores have an extremely thick spore coat
16 terms
The Spore Stain
Explain the term vegetative as it appl…
Why are spores more difficult to stain…
Why are spores refractile?
Explain the terms sporulation and germ…
active, living metabolized cells
spores have an extremely thick spore coat
Because it has many layers,, light will bounce off of it
sporulation: the production of the endospore within the veget…
Explain the term vegetative as it appl…
active, living metabolized cells
Why are spores more difficult to stain…
spores have an extremely thick spore coat
8 terms
Spore staining
What is the primary stain?
What is the mordant?
What is the decolorizer?
What is the counterstain?
Malachite green
Steam
Water
Safranin
What is the primary stain?
Malachite green
What is the mordant?
Steam
14 terms
spore staining
sporulation
core of endospore
endospores have multiple layers called
primary stain
turning into endospore; takes about 12 hrs; different bacteri…
dehydrated cytoplasm
exospore
malachite green
sporulation
turning into endospore; takes about 12 hrs; different bacteri…
core of endospore
dehydrated cytoplasm
14 terms
Spore Staining
species of bacteria that form spores
functions of endospores
germination
exosporium
Bacillus and Clostridium
-resting stages... -allow bacteria to survive unfavorable growth…
return to vegetative cell
-protein coat surrounding endospore resulting in resistance…
species of bacteria that form spores
Bacillus and Clostridium
functions of endospores
-resting stages... -allow bacteria to survive unfavorable growth…
11 terms
Spore Staining
What is the color of the vegetative ce…
What is the secondary stain in the spo…
What is the color of endospores after…
What is the color of endospores after…
red
Safranin is the counterstain that will give color to the colo…
Spores will not be penetrated by the gram stain and so will a…
If performed properly, the primary stain, malachite green, wi…
What is the color of the vegetative ce…
red
What is the secondary stain in the spo…
Safranin is the counterstain that will give color to the colo…
12 terms
Spore Staining
Of the three genera of bacteria, which…
What is the color of the vegetative ce…
What is the secondary stain in the spo…
What is the color of endospores after…
Mycobacterium is an acid-fast bacterium, not an endospore pro…
Again, the counterstain safranin will color the vegetative ce…
Safranin is the counterstain that will give color to the colo…
Spores will not be penetrated by the gram stain and so will a…
Of the three genera of bacteria, which…
Mycobacterium is an acid-fast bacterium, not an endospore pro…
What is the color of the vegetative ce…
Again, the counterstain safranin will color the vegetative ce…
9 terms
Spore Stain Technique
1. make a smear and allow to air dry
2. gently heat fix
3. Spore stain - Primary dye... - stains…
4. remove from heat, dispose of paper…
start boiling water in a flask
...
Malachite Green... place a piece of paper towel on top of the sl…
...
1. make a smear and allow to air dry
start boiling water in a flask
2. gently heat fix
...
Spore Stain and Acid Fast Stain
spore... one... one life... cell... spore
reproductive... survival
dessication... chemical... nutrient depletio…
keratin
A ____________ is a resistant structure produced by a vegetat…
a spore is NOT a ________________ structure, it is a ________…
Spores are resistant to:... D_______________________ (when the v…
Resistive Function of Spores... ___________________ - surrounds…
spore... one... one life... cell... spore
A ____________ is a resistant structure produced by a vegetat…
reproductive... survival
a spore is NOT a ________________ structure, it is a ________…
Spore Staining, Acid Fast stain
endospore
Bacillus subtiulus,Bacillus megaterium…
Germination
Exosporium
a complex developmental cycle that produces resting stages. I…
bacteria belonging to these genera create endospores
a process that takes place if nutrients become favorable agai…
a protein coat that causes resistance by forming a protective…
endospore
a complex developmental cycle that produces resting stages. I…
Bacillus subtiulus,Bacillus megaterium…
bacteria belonging to these genera create endospores
16 terms
#9 Spore Stain
What genus are capable of existing met…
What is genus clostridium?
What is genus mycobacterium?
What are the two pathogenic endospores?
Genus Clostridium (Anaerobic) ... Genus Bacillus (Aerobic)
Anaerobic
Aerobic
Bacillus anthracis and Clostridium botulinum
What genus are capable of existing met…
Genus Clostridium (Anaerobic) ... Genus Bacillus (Aerobic)
What is genus clostridium?
Anaerobic
8 terms
Spore Stain Procedure
1
2
3
4
Make Smear
Add MALACHITE GREEN to smear and microwave (heat mordant) for…
Cool, rinse with water.
Add SAFRANIN for 30-45 seconds
1
Make Smear
2
Add MALACHITE GREEN to smear and microwave (heat mordant) for…
8 terms
exercise 17- spore stain
what type of stain is spore stain
what are you staining
how to see spores in gram or simple st…
how the spore stain works
a selective stain
the endospore itself
as clear (unstained) areas inside a vegetative cell
•drives a readily water-soluble dye (malachite green) into th…
what type of stain is spore stain
a selective stain
what are you staining
the endospore itself
16 terms
Lab 4 The Spore Stain
Explain the term vegetative as it appl…
Why are spores more difficult to stain…
Why are spores refractile?
Explain the terms sporulation and germ…
active, living metabolized cells
spores have an extremely thick spore coat
Because it has many layers,, light will bounce off of it
sporulation: the production of the endospore within the veget…
Explain the term vegetative as it appl…
active, living metabolized cells
Why are spores more difficult to stain…
spores have an extremely thick spore coat
11 terms
lab 8a spore stain
bacterial species that produce endospore
concept of endospores
what happens to bacteria with endospor…
endospores have ____ structures that a…
bacillus and clostridium
endospore is formed when faces environmental adversity... sporu…
spore may germinate to form a new vegetative cell and growth…
dehydrated... metabolically
bacterial species that produce endospore
bacillus and clostridium
concept of endospores
endospore is formed when faces environmental adversity... sporu…
7 terms
Spore and Acid-Fast Stain
What is the purpose of Spore staining?
Describe the structure of an endospore.
What bacteria can form endospores?
Why are you able to use a single organ…
is used to distinguish organisms capable of forming endospores
spore coat provides is resistant to many forms of damage and…
Gram positive bacteria
bc vegetative cells and endospores come from each other thus…
What is the purpose of Spore staining?
is used to distinguish organisms capable of forming endospores
Describe the structure of an endospore.
spore coat provides is resistant to many forms of damage and…
38 terms
Acid fast and spore staining
cytological basis for acid-fast differ…
mycolic acid
ziehl-neelson method
kinyoun method
prescence of mycolic acids in cell walls of acid fast organisms
waxy substance that gives af organisms higher affinity for pr…
uses heat as part of staining process
does not use heat is "cold" staining
cytological basis for acid-fast differ…
prescence of mycolic acids in cell walls of acid fast organisms
mycolic acid
waxy substance that gives af organisms higher affinity for pr…
7 terms
list 4 steps in spore stain
1 st step in spore stain
2nd step in spore stain
3rd step in spore stain
4th step in spore stain
boil water
malachite green 5 min
water rinse
safranin 2 min
1 st step in spore stain
boil water
2nd step in spore stain
malachite green 5 min
6 terms
Spore staining (exercise 15)
endospores
are endospores hydrated or dehydrated?
what accounts for an endospore's resis…
the higher the water content of an end…
resting stage; non vegetative cell; allow bacteria to survive…
DEhydrated; do not actively metabolize; resistant to heat, ra…
the protein coat, exosporium, that forms a protective barrier…
LESS
endospores
resting stage; non vegetative cell; allow bacteria to survive…
are endospores hydrated or dehydrated?
DEhydrated; do not actively metabolize; resistant to heat, ra…
22 terms
endospore/spore staining procedure
what 2 genus' are really good spore fo…
what 4 medical issues can arise from s…
what are spores resistant to?
what is a vegetative cell?
1. Bacillus ... 2. Clostridium
1. botulism... 2. tetanus... 3. anthrax... 4. gangrene
heat, chemicals, and radiation
metabolically active cell that forms endospores
what 2 genus' are really good spore fo…
1. Bacillus ... 2. Clostridium
what 4 medical issues can arise from s…
1. botulism... 2. tetanus... 3. anthrax... 4. gangrene
6 terms
exercise 8 spore staining
endospores
germination
exosporium
autoclave
allow bacteria to survive conditions that are not favorable f…
forms vegetative cells
protein coat, protective barrier around spore ... makes endospor…
kills endospores
endospores
allow bacteria to survive conditions that are not favorable f…
germination
forms vegetative cells
10 terms
Lab 16: Spore Staining
What are the functions of endospores i…
What external structure on the endospo…
Compared to a vegetative cell, how muc…
What is the mordant in the spore stain?
Endospores allow bacteria to live in a dormant state in order…
The exosporium acts as a barrier. It has heat resistance that…
10-30%
Heat
What are the functions of endospores i…
Endospores allow bacteria to live in a dormant state in order…
What external structure on the endospo…
The exosporium acts as a barrier. It has heat resistance that…
20 terms
Microbiology Spore Staining
Why are thick or dense smears less lik…
Why is it essential that smears be air…
Why should you be careful not to overh…
Why do you think the presence of greas…
It will diminish the amount o flight that can pass through ma…
1. Overheat will denature and rupture the cell wall. 2. exces…
Overheating will distort the morphology through plasmolysis o…
1. Bacteria will attach to the grease or dirt and wash away w…
Why are thick or dense smears less lik…
It will diminish the amount o flight that can pass through ma…
Why is it essential that smears be air…
1. Overheat will denature and rupture the cell wall. 2. exces…
14 terms
Exercise 15- Spore Staining
When species of bacteria belong to the…
What do endospores do?
What are four things endospores are re…
The endospores resistance is due to a…
Endospores
Allow bacteria to survive in unfavorable environmental condit…
-Heat... -Radiation... -Acids... -Disinfectants
Exosporium
When species of bacteria belong to the…
Endospores
What do endospores do?
Allow bacteria to survive in unfavorable environmental condit…
14 terms
micro lab 12 spore stain
Bacillus and Clostridium
endospore
central spore
terminal spore
Which two bacteria produce spores?
Spore structure is made inside the cell and called _____
spore in the "middle" of the rod
spore at the end of the rod
Bacillus and Clostridium
Which two bacteria produce spores?
endospore
Spore structure is made inside the cell and called _____
28 terms
Micro Lab 4 Spore Stain
Bacillus subtilus
Bacillus sterothermophilus
Bacillus cereus
Bacillus anthracis
common lab contaminent... lives in dust and soil... grows as fros…
high heat loving bug... used in the medical field to quality co…
causes vomiting and diarrhea... associated with food contaminat…
causes anthrax... Black Plague... black as coal
Bacillus subtilus
common lab contaminent... lives in dust and soil... grows as fros…
Bacillus sterothermophilus
high heat loving bug... used in the medical field to quality co…
18 terms
spore stain capsule stain and motility (4)
what is the endospore stain used for?
why is steam used in an endospore stain?
what is used at the primary stain in a…
what stains are used in a capsule stain
to distinguish between inclusions such as starch, sulfur, and…
because the spores are so resistant
steam
negatively charged stains or a specialized positive stain
what is the endospore stain used for?
to distinguish between inclusions such as starch, sulfur, and…
why is steam used in an endospore stain?
because the spores are so resistant
14 terms
Lab 3: Gram Stain and Spore Stain
To prepare a Gram Stain
Steps for gram stain
Steps for endospore stain
Which structural difference between gr…
1. Make a smear from a broth or agar then heat fix... 2. Flood s…
1. Primary stain- crystal violet (Stains both +- purple)... 2.Mo…
1. Primary stain- Malachite green-enters cells not spores... 2.H…
Gram neg are more clear with pink in the middle (rods) struct…
To prepare a Gram Stain
1. Make a smear from a broth or agar then heat fix... 2. Flood s…
Steps for gram stain
1. Primary stain- crystal violet (Stains both +- purple)... 2.Mo…
9 terms
spore stain (Schaeffer-Fulton Method)
Which gram postive bacteria can tranfo…
What does the application of high heat…
What is the primary stain in the spore…
Is malachite green water-soluble?
Primarily, genera Bacillus and Clostridium
The high heat can force the primary stain, malachite green in…
malachite green
Yes, and therefore, washes out of vegetative cells in the sme…
Which gram postive bacteria can tranfo…
Primarily, genera Bacillus and Clostridium
What does the application of high heat…
The high heat can force the primary stain, malachite green in…
42 terms
141L - Gram+ Bacillus / Spore Stain
What are some general characteristics…
What is the advantage of having spores?
What is Bacillus often confused with?…
What is the most medically relevant Ba…
Found in water/soil, Gram+ or Gram variable, Catalase+, form…
Resistant to conditions to which vegetative cells are intoler…
They are confused with Clostridium because of the endospores.…
Bacillus cereus is the most medically relevant Bacillus group…
What are some general characteristics…
Found in water/soil, Gram+ or Gram variable, Catalase+, form…
What is the advantage of having spores?
Resistant to conditions to which vegetative cells are intoler…
10 terms
Lab 16 Spore Staining Questions
What are the functions of endospores i…
a. What external structure on the endo…
Compared to a vegetative cell, how muc…
What can be considered the mordant in…
protection
a. exosporium... b. protein coat
10-30%
heat
What are the functions of endospores i…
protection
a. What external structure on the endo…
a. exosporium... b. protein coat
9 terms
Differential Staining Techniques Acid-fast and Spore Stains
Name two acid-fast species
List the reagents utilized in the Kiny…
Contrast the advantages and disadvanta…
Explain why acid-fast bacteria are gra…
In Lab: Mycobacterium Phlei and Mycobacterium tuberculosis... In…
Carbolfuchsin is used as primary stains because it's lipid... so…
--Kinyoun is useful for organisms that stain weakly or are pa…
The spirochetes (treponema, borrelia, leptospira, spirillum)…
Name two acid-fast species
In Lab: Mycobacterium Phlei and Mycobacterium tuberculosis... In…
List the reagents utilized in the Kiny…
Carbolfuchsin is used as primary stains because it's lipid... so…
35 terms
Micro Lab Spore Stain and Bacteria Motility
What genera of bacteria are capable of…
What is the primary stain in a spore s…
What is the mordant in a spore stain?
What is the decolorizer in a spore sta…
6 genera of spore forming organisms - most are gram+ rod-shap…
malachite green
heat
water
What genera of bacteria are capable of…
6 genera of spore forming organisms - most are gram+ rod-shap…
What is the primary stain in a spore s…
malachite green
6 terms
MIC 103L Lab Practical Microscopes and spore stain
fine and coarse focus knobs
field diaphragm control
condenser focus knob
condenser centering screws
move stage and condenser
open or closes field diaphragm
moves just condenser
physically move condenser lens assembly
fine and coarse focus knobs
move stage and condenser
field diaphragm control
open or closes field diaphragm
13 terms
Microbio Lab Exercise 17: Spore Stain
What type of stain is spore staining?
What is hard to stain?
Endospore can be seen in gram and simp…
What dye is derived into the spore by…
a selective stain
endospore
clear (unstained)
malachite green
What type of stain is spore staining?
a selective stain
What is hard to stain?
endospore
Lab 4-Acid Fast and Spore Stain
Fastness
Acid-Fast Staining( Ziehl-Neelsen meth…
Acid-Fast aka Acid-Fast Positive Cells
Acid-Fast Negative Cells
-Ability to retain dye when challenged by a decolorizer
Procedure:... -CARBOL FUSHIN Primary dye used with steam... -Acid a…
-Retain carbol fushin dye when challenged with Acid Alcohol d…
-Is readily decolorized and counter-stained with methylene dy…
Fastness
-Ability to retain dye when challenged by a decolorizer
Acid-Fast Staining( Ziehl-Neelsen meth…
Procedure:... -CARBOL FUSHIN Primary dye used with steam... -Acid a…
26 terms
BIOL 240L Experiment #4-8: gram stain, acid-fast stain, spore stain, negative stain, capsule stain
Differential staining techniuqe
Primary stain in gram stain
Mordant in general/mordant used in Gra…
Counterstain in Gram stain
Used to differentiate bacteria depending on their abilities t…
Crystal violet
Mordant = a substance that fixes the primary stain in the bac…
Safranin
Differential staining techniuqe
Used to differentiate bacteria depending on their abilities t…
Primary stain in gram stain
Crystal violet
15 terms
Microbiology Lab 5 (Acid Fast and Spore Stain)
What kind of stain is an acid stain?
What kind of bacteria does this descri…
What important pathogens are acid-fast?
Why are acid fast bacteria stained red?
A differential stain
Acid fast
Mycobacterium tuberculosis and Mycobacterium leprae
Acid fast bacteria retain the red color due to carbolfuchsin…
What kind of stain is an acid stain?
A differential stain
What kind of bacteria does this descri…
Acid fast
15: Spore Staining: Schaeffer-Fulton Method
Endospores
Exosporium
Heat resistance
Destroying endospore
-allow bacteria to survive unfavorable conditions ... -dehydrate…
-protein coat around endospore that forms protective barrier
higher water content = less heat resistant
-15-20 minutes at 121 degrees C
Endospores
-allow bacteria to survive unfavorable conditions ... -dehydrate…
Exosporium
-protein coat around endospore that forms protective barrier
22 terms
Micro Lab Experiment #11 Spore and Capsule stain
Schaeffer-Fulton Method
Clostridium and Desulfotomaculum, and…
Vegetative Cells
Spores
Spore stain
Examples of organisms that have the capacity to exist either…
Metabolically active cells
Highly resistant, metabolically inactive cells.
Schaeffer-Fulton Method
Spore stain
Clostridium and Desulfotomaculum, and…
Examples of organisms that have the capacity to exist either…
54 terms
Micro Lab 4 Spore Stain and Motility Stabs
Differentiates between organisms that…
The spore stain uses what as a mordant…
Bergey's Manual lists how many genera…
Most of the genera of spore forming or…
Differential Stain
Heat as a mordant
6
Gram positive rod shaped
Differentiates between organisms that…
Differential Stain
The spore stain uses what as a mordant…
Heat as a mordant
23 terms
Lab Gram staining, spore staining, and acid fast staining
Gram staining procedure
Spore staining procedure
acid fast procedure
Stains used in gram staining
Gram Staining Procedure ( AGA DG CGB)... 1.Add crystal violet fo…
Spore Staining Procedure ( CS2 DAC GB)... 1.Cover the smear prep…
Acid- Fast Procedure C SAD GCGB... 1.Cover smear with small piec…
*Crystal Violet primary stain 20 seconds... * Mordant- Gram's Io…
Gram staining procedure
Gram Staining Procedure ( AGA DG CGB)... 1.Add crystal violet fo…
Spore staining procedure
Spore Staining Procedure ( CS2 DAC GB)... 1.Cover the smear prep…
31 terms
experiment #3 spore and acid fast stain
what are the objectives for spore stain?
what are the objectives for acid fast…
the spore stain does what?
spores are what?
-to learn how to do the spore stain and to determine if the b…
- to learn how to do an acid fast stain and to determine if t…
demonstrates that the clear area in a gram stained or simple…
refractive ( don't absorb) to stains, therefore heat is neces…
what are the objectives for spore stain?
-to learn how to do the spore stain and to determine if the b…
what are the objectives for acid fast…
- to learn how to do an acid fast stain and to determine if t…
17 terms
Micro LAB: Chapter 15, Spore Staining
Endospores
Exosporium
Heat resistance
Sporulation
Allow bacteria such as Bacillus & Clostridia to survive envir…
A protein coat that adds resistance as a protective barrier a…
Associated with the water content of endospores... The higher th…
Water content is reduced 10-30% of the vegetative cell.... Calci…
Endospores
Allow bacteria such as Bacillus & Clostridia to survive envir…
Exosporium
A protein coat that adds resistance as a protective barrier a…
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