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Study sets matching "term:msa = mannitol salt agar"

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Study sets matching "term:msa = mannitol salt agar"

MSA mannitol salt agar
What does MSA contain?
Why is Phenol red added?
Is MSA medium selective or differential?
Why is mannitol added?
-carbohydrate mannitol... -7.5% sodium chloride (NaCl)... -Phenol red
It's a pH indicator of fermentation... -red turns yellow below…
Both
Provides the substrate for fermentation and make medium diffe…
What does MSA contain?
-carbohydrate mannitol... -7.5% sodium chloride (NaCl)... -Phenol red
Why is Phenol red added?
It's a pH indicator of fermentation... -red turns yellow below…
Mannitol Salt Agar (MSA)
What is the ONLY bacteria that will gr…
What is the Mannitol Salt Agar (MSA) c…
Mannitol Salt Agar (MSA) inhibits the…
Mannitol Salt Agar (MSA) is differenti…
Staphylococcus G+
7.5% NaCl & Mennitol fermenter
True
Mannitol Fermentation
What is the ONLY bacteria that will gr…
Staphylococcus G+
What is the Mannitol Salt Agar (MSA) c…
7.5% NaCl & Mennitol fermenter
MSA Mannitol Salt Agar
Does Vibrio anguillarum exhibit no/poo…
Does Bacillus cereus exhibit no/poor g…
Does Klebsiella pneumoniae exhibit no/…
Does Micrococcus luteus exhibit no/poo…
Poor growth. Inhibited by NaCl. Not Staphylococcus.
Poor growth. Inhibited by NaCl. Not Staphylococcus.
Poor growth. Inhibited by NaCl. Not Staphylococcus.
Poor growth. Inhibited by NaCl. Not Staphylococcus.
Does Vibrio anguillarum exhibit no/poo…
Poor growth. Inhibited by NaCl. Not Staphylococcus.
Does Bacillus cereus exhibit no/poor g…
Poor growth. Inhibited by NaCl. Not Staphylococcus.
7 terms
Mannitol salt agar (MSA)
Differential for what bacteria
carbon source
selective agent
Why is it considered selective
staphlococci
carbohydrate mannitol
7.5% NaCL
because of such high salt concentration
Differential for what bacteria
staphlococci
carbon source
carbohydrate mannitol
Mannitol Salt Agar (MSA)
What kind of plate?
Used for which genus:
What are the agents included? why?
pH indicator:
Selective and Differential
to ID species of staphylococci
7.5% NaCl-selects for halotolerent bacteria... Mannitol (differe…
phenol red
What kind of plate?
Selective and Differential
Used for which genus:
to ID species of staphylococci
8 terms
Mannitol Salt Agar (MSA)
Selective? Enriched? Differential?
Selective agent
Differential for?
Indicator of fermentation
Selective and differential
7.5% NaCl: inhibits colt intolerant bacteria
Mannitol fermentation: bacteria ferment mannitol to an acid a…
Media has phenol red ... low pH (>7) (acid)- bright yellow ... Neut…
Selective? Enriched? Differential?
Selective and differential
Selective agent
7.5% NaCl: inhibits colt intolerant bacteria
9 terms
Mannitol Salt Agar (MSA)
What is it used for?
7.5% sodium chloride
pH indicator is what color and what is…
when is it yellow?
isolation and differentiation of Staphylococcus aures and fro…
makes the medium selective because its concentration is high…
red at pH 7.4-8.4
when the pH is below pH 6.8
What is it used for?
isolation and differentiation of Staphylococcus aures and fro…
7.5% sodium chloride
makes the medium selective because its concentration is high…
9 terms
MSA (Mannitol Salt Agar) Plate
Mannitol Salt Agar
Red
True
For comparison between Negative (-) Re…
What does MSA stand for?
What is the color of the agar plate, for MSA?
T or F: We inoculated half of the MSA plate.
Why do we inoculate half of an agar plate?
Mannitol Salt Agar
What does MSA stand for?
Red
What is the color of the agar plate, for MSA?
10 terms
Mannitol Salt Agar plate (MSA)
7.5%
Halophiles
mannitol fermentation
pH ↓
what percentage of salt is found on the MSA?
what is the MSA selective for?
what is the MSA differential for?
if the bacteria does ferment a sugar what happens to pH?
7.5%
what percentage of salt is found on the MSA?
Halophiles
what is the MSA selective for?
11 terms
Lab Exam 2 - Mannitol Salt Agar (MSA)
What does the Mannitol Salt Agar conta…
What is the purpose of Carbohydrate Ma…
What is the purpose of NaCl ?
Why does staphylococcus thrive in this…
- Carbohydrate Mannitol... - Sodium Chloride (NaCl)... - Phenol Red
- it is a substrate for fermentation ... - makes the medium diff…
- kills most bacteria that aren't tolerate to NaCl (salt)
because staphylococcus like salty habitats (like that of the…
What does the Mannitol Salt Agar conta…
- Carbohydrate Mannitol... - Sodium Chloride (NaCl)... - Phenol Red
What is the purpose of Carbohydrate Ma…
- it is a substrate for fermentation ... - makes the medium diff…
13 terms
Lab #27 (Mannitol Salt Agar) MSA
Mannitol salt agar plates
Example of Pathogenic Staphylococcus
Example of non-pathogenic Staphylococcus
Is Mannitol salt selective or differen…
used to help identify pathogenic Staphylococcus species from…
S. aureus
S. epidermis
BOTH
Mannitol salt agar plates
used to help identify pathogenic Staphylococcus species from…
Example of Pathogenic Staphylococcus
S. aureus
10 terms
Exercise 4-4: Mannitol Salts Agar (MSA)
Mannitol Salts Agar: Used to help iden…
Important components and functions in…
Mannitol (carbohydrate)
Phenol Red (pH indicator)
Staphylococcus
- 7.6% Salt - Selective ... - Mannitol (carbohydrate) ... - Phenol…
differential ... - only pathogens can ferment Mannitol- acid pro…
differential
Mannitol Salts Agar: Used to help iden…
Staphylococcus
Important components and functions in…
- 7.6% Salt - Selective ... - Mannitol (carbohydrate) ... - Phenol…
5 terms
Mannitol Salt Agar
mannitol salt agar... selective for staph…
selective and differential... 7.5% NaCI a…
phenol red
pathogenic staphylococci FM/non-pathog…
What is the name of this test ?... What is the purpose?
What is the media used?... What is the substrate?
What is the pH indicator?
Why is mannitol sugar important?
mannitol salt agar... selective for staph…
What is the name of this test ?... What is the purpose?
selective and differential... 7.5% NaCI a…
What is the media used?... What is the substrate?
10 terms
Micro Lab Mannitol Salt Agar (MSA) Quiz 2
Purpose of Mannitol Salt Agar
Staphylococci
Staphylococcus epidermidis
Selective component
This medium is a selective and differential medium used to gr…
Is used to grow on media containing high concentration (7.5%)…
bacteria that grows on our skin and have adapted to a salty e…
High Salt concentration
Purpose of Mannitol Salt Agar
This medium is a selective and differential medium used to gr…
Staphylococci
Is used to grow on media containing high concentration (7.5%)…
8 terms
Blood Agar & Mannitol Salt Agar
mannitol salt agar... selective for staph…
selective and differential... 7.5% NaCI a…
phenol red
pathogenic staphylococci FM/non-pathog…
What is the name of this test ?... What is the purpose?
What is the media used?... What is the substrate?
What is the pH indicator?
Why is mannitol sugar important?
mannitol salt agar... selective for staph…
What is the name of this test ?... What is the purpose?
selective and differential... 7.5% NaCI a…
What is the media used?... What is the substrate?
8 terms
Mannitol Salt Agar
What does MSA stand for?
Is MSA selective or differential?
What percentage of NaCl does the mediu…
What organism does the medium inhibit?…
Mannitol Salt Agar
Both
7.5% NaCl
Gram-negative organisms
What does MSA stand for?
Mannitol Salt Agar
Is MSA selective or differential?
Both
11 terms
Mannitol Salt Agar
Define Selective Media
Define Differential Media
What is the purpose of Mannitol Salt A…
Is Mannitol Salt Agar a selective or d…
This media suppresses the growth of unwanted organisms and en…
This media allows you to differentiate between 2 colonies of…
It inhibits the growth of all gram - organisms and nonstaphyl…
It is both selective and differential
Define Selective Media
This media suppresses the growth of unwanted organisms and en…
Define Differential Media
This media allows you to differentiate between 2 colonies of…
11 terms
Week6 4-4 Mannitol Salt Agar (MSA)
Phenol Red Colors at pHs (MSA)
Purpose of Mannitol (MSA)
Purpose of Sodium Chloride (MSA)
What thrives in the MSA medium?
Below 6.8 - Yellow... Between 7.4 to 8.4 - Red... Above 8.4 - Pink
Provides the substrate for fermentation and makes the medium…
Makes the medium selective because it's concentration is high…
Staphylococci because of their adaptations to salty environme…
Phenol Red Colors at pHs (MSA)
Below 6.8 - Yellow... Between 7.4 to 8.4 - Red... Above 8.4 - Pink
Purpose of Mannitol (MSA)
Provides the substrate for fermentation and makes the medium…
8 terms
Mannitol Salt agar
Mannitol salt is both...
Selective media....
How is mannitol salt agar selective?
The concentration of mannitol salt is...
Selective and differential
Allows for the growth of specific bacteria, but inhibits others
Due to high salt concentration, it allows halophiles to grow,…
7.5% NaCl
Mannitol salt is both...
Selective and differential
Selective media....
Allows for the growth of specific bacteria, but inhibits others
17 terms
Mannitol Salt Agar
Mannitol Salt Agar
Osmotolerant
Sodium Chloride
D-Mannitol
Presumptively identifies Staphylococcus aureus and selects fo…
Salt tolerant (Halophile)
7.5%... Selective for osmotolerant organisms
Differential ... Sugar that looks for fermenters
Mannitol Salt Agar
Presumptively identifies Staphylococcus aureus and selects fo…
Osmotolerant
Salt tolerant (Halophile)
11 terms
Mannitol Salt Agar
what is a selective medium?
Explain how mannitol salt agar is sele…
What is a differential medium?
explain how mannitol salt agar is diff…
growth medium that favors growth of one group of microorganis…
the sodium chloride in mannitol salt agar makes the medium se…
growth medium that contains an indicator to detect the presen…
mannitol provides the substrate for fermentation making it di…
what is a selective medium?
growth medium that favors growth of one group of microorganis…
Explain how mannitol salt agar is sele…
the sodium chloride in mannitol salt agar makes the medium se…
8 terms
Mannitol Salt Agar
Medium Recipes
Indicator
pH indicator
Purpose
7.5% Sodium chloride, Mannitol, Phenol red
Phenol red
yellow bellow 6.8... red between 7.4-8.4... pink at 8.4... occurs thro…
isolation of Staphylococcus aureus
Medium Recipes
7.5% Sodium chloride, Mannitol, Phenol red
Indicator
Phenol red
12 terms
MANNITOL SALT AGAR TEST
What kind of medium?
Ingredients:
Purpose of NaCl:
Purpose of mannitol:
Selective and differential.
NaCl, mannitol, and phenol red.
Selects staphylococci, because they adapt well to salty envir…
Provides substrate for fermentation.
What kind of medium?
Selective and differential.
Ingredients:
NaCl, mannitol, and phenol red.
9 terms
Mannitol Salt Agar
What is makes up mannitol salt agar?
What compound makes MSA differential?
What compound makes MSA selective?
What color will phenol red turn if man…
beef extract, D-mannitol, 7.5 percent sodium chloride, pepton…
the mannitol precent differentiates between mannitol fermento…
The sodium chloride (a salt) only allows Staphylococcus to gr…
The media will turn yellow due to acid byproducts present as…
What is makes up mannitol salt agar?
beef extract, D-mannitol, 7.5 percent sodium chloride, pepton…
What compound makes MSA differential?
the mannitol precent differentiates between mannitol fermento…
Mannitol Salt Agar
Purpose
Recipe
what is the salt concentration of the…
pH indicator
to select for the growth of salt-tolerant microbes and presum…
beef extract and peptones... sodium chloride @ 7.5%... Mannitol Sug…
7.5%
phenol red
Purpose
to select for the growth of salt-tolerant microbes and presum…
Recipe
beef extract and peptones... sodium chloride @ 7.5%... Mannitol Sug…
9 terms
mannitol salt agar
What does no growth indicate
what does good growth indicate
what does yellow growth or halo indicate
what does red growth indicate
organism is inhibited by NaCl
organism is not inhibited by NaCl
organism produces acid from mannitol fermentation
organism does not ferment mannitol, no rxn
What does no growth indicate
organism is inhibited by NaCl
what does good growth indicate
organism is not inhibited by NaCl
10 terms
mannitol salt agar
how does it look originally?
straight or slant?
solid or liquid?
what do you expect to see?
...
...
...
...
how does it look originally?
...
straight or slant?
...
10 terms
Mannitol Salt Agar
Mannitol Salt Agar
Phenol Red
Mannitol
NaCl in Mannitol Salt Test
Selective and differenital... Contains:... - carb mannitol (what m…
Yellow = below 6.8... Pink = Above 8.4... Red = inbetween
provides substrate for fermentation ... makes substrate differe…
concentration high enough to kill and dehydrate most bacteria…
Mannitol Salt Agar
Selective and differenital... Contains:... - carb mannitol (what m…
Phenol Red
Yellow = below 6.8... Pink = Above 8.4... Red = inbetween
5 terms
Mannitol Salt Agar Quiz
What sugar is in MSA?
"Who" ferments it? As in whom are we l…
What is the differential agent (chemic…
What color is a positive reaction?
Mannitol
Staphylococcus aureus; gram positive staph
Phenol red
Yellow
What sugar is in MSA?
Mannitol
"Who" ferments it? As in whom are we l…
Staphylococcus aureus; gram positive staph
10 terms
Mannitol Salt Agar
What type of organism is the media tes…
Is the medium selective or differential?
What makes the test selective?
What makes the test differential?
Isolating Staphylococcus aureus from other staphylococci
Both
Sodium chloride is the selective agent, high salt concentrati…
Mannitol (also substrate)- test whether or not an organism ca…
What type of organism is the media tes…
Isolating Staphylococcus aureus from other staphylococci
Is the medium selective or differential?
Both
6 terms
Mannitol Salt Agar
What makes the medium differential?
What makes the medium selective?
What is the pH indicator?
What color does the pH change to and a…
Mannitol and phenol red
7.5% NaCl
phenol red
yellow with pH of 6.8 or lower indicates the the organism was…
What makes the medium differential?
Mannitol and phenol red
What makes the medium selective?
7.5% NaCl
6 terms
Mannitol Salt Agar
mannitol agar
what purpose does the nutrient agar pl…
what would be the likely consequences…
would omitting the NaCl alter the medi…
used to isolate and differentiate staphylococcus aureus
nutrient agar plate serves as control; it shows that gram neg…
all the organisms would grow equally because salt is the sele…
it would alter the specificity because all bacteria would gro…
mannitol agar
used to isolate and differentiate staphylococcus aureus
what purpose does the nutrient agar pl…
nutrient agar plate serves as control; it shows that gram neg…
10 terms
Mannitol Salt Agar
What are the main constituents in Mann…
What is Mannitol Salt Agar (MSA) used…
What does the Mannitol contained in MS…
What does the NaCl contained in MSA do?
-The carbohydrate Mannitol... -7.5% NaCl... -Phenol Red (pH indicat…
Isolation and differentiation of Staphylococcus aureus.
Provides the substrate for fermentation and makes the medium…
Makes the medium selective because its concentration is high…
What are the main constituents in Mann…
-The carbohydrate Mannitol... -7.5% NaCl... -Phenol Red (pH indicat…
What is Mannitol Salt Agar (MSA) used…
Isolation and differentiation of Staphylococcus aureus.
10 terms
Mannitol Salts Agar
What is the purpose of this test?
Is this test selective or differential?
Why is the test considered selective?
Why is the test considered differential?
Used for isolation and differentiation of Staphyloccus auerus
Selective and differential
Because of the high salt content that discourages the growth…
Because the mannitol and the phenol red make the medium diffe…
What is the purpose of this test?
Used for isolation and differentiation of Staphyloccus auerus
Is this test selective or differential?
Selective and differential
14 terms
Mannitol Salts Agar
1 Mannitol- carbohydrate 2 Sodium chlo…
1 Yellow below pH6.8, 2 Red at pH 7.4-…
Mannitol (carbohydrate) provides the s…
its concentration is high enough to de…
What is in the media Mannitol Salts Agar & its functions
What are the colors of the pH indicator phenol red in Mannito…
What makes the medium differential in Mannitol salts agar?
Sodium chloride make the medium selective because
1 Mannitol- carbohydrate 2 Sodium chlo…
What is in the media Mannitol Salts Agar & its functions
1 Yellow below pH6.8, 2 Red at pH 7.4-…
What are the colors of the pH indicator phenol red in Mannito…
22 terms
Micro Lab: Mannitol Salt Agar
Staphylococcus epidermidis ... Streptococ…
Staphylococcus
Streptococcus
Gram positive
Common skin bacteria of clinical importance include
What organism is this?
What organism is this?
Staphylococcus and Streptococcus both have what gram stain?
Staphylococcus epidermidis ... Streptococ…
Common skin bacteria of clinical importance include
Staphylococcus
What organism is this?
9 terms
Mannitol salt agar (lab 22)
medium
substrate
reagents
positive end products ?
MSA Plate
mannitol (alcohol sugar), 7.5% and up high salt concentration
n/a
acidic waste
medium
MSA Plate
substrate
mannitol (alcohol sugar), 7.5% and up high salt concentration
6 terms
4-3: Mannitol Salt Agar
Mannitol Salt Agar (MSA)
Yellow
Red
Pink
This agar contains the carbohydrate mannitol, 7.5% sodium chl…
Phenol red is this color below pH 6.8
Phenol red is this color at pH 7.4 and 8.4
Phenol red is this color at pH 8.4 and above.
Mannitol Salt Agar (MSA)
This agar contains the carbohydrate mannitol, 7.5% sodium chl…
Yellow
Phenol red is this color below pH 6.8
Quiz 4 Mannitol salt agar
Mannitol salt agar color
Selective or not? MSaltA
Contains what? Salt agar
What kind of bacteria can grow? Salt a…
Pink
Selective
7.5% salt
Halophilic, haloduric, staph epidermis
Mannitol salt agar color
Pink
Selective or not? MSaltA
Selective
22 terms
Micro Lab mannitol salt agar
For clinical purposes, correct identif…
The Human Genome Project is designed t…
Functional media enable the growth of…
General purpose media restrict most or…
True
false
True
False
For clinical purposes, correct identif…
True
The Human Genome Project is designed t…
false
12 terms
Microbiology Lab Mannitol Salt Agar
MSA contains what 3 things
below what pH is phenol red yellow?
pink at what pH?
red btw what pH's
carbohydrate manitol, sodium chloride (NaCl), and pH indicato…
below pH 6.8
pH 8.4 and above
7.4-8.4
MSA contains what 3 things
carbohydrate manitol, sodium chloride (NaCl), and pH indicato…
below what pH is phenol red yellow?
below pH 6.8
9 terms
4.3 Mannitol Salt Agar
Can be used to select for
Selective
Differential
Refer to Microbio MSA Pic - ID the thr…
Gram-Positive Cocci... e.g. E. coli
Uses high salt concentrations... E. coli = -
Differentiates bacteria on the basis of their ability to ferm…
A: Staph aureus... B: Staph epidermidis... C. E. coli
Can be used to select for
Gram-Positive Cocci... e.g. E. coli
Selective
Uses high salt concentrations... E. coli = -
3 GML - Mannitol Salt Agar
mannitol salt agar contains what 3 thi…
color of pH below 6.8 (MSA)
color of pH 7.4-8.4 (MSA)
color of pH above 8.4 (MSA)
1. carbohydrate mannitol 2. 7.5% sodium chloride 3. pH indica…
yellow
red
pink
mannitol salt agar contains what 3 thi…
1. carbohydrate mannitol 2. 7.5% sodium chloride 3. pH indica…
color of pH below 6.8 (MSA)
yellow
5 terms
Micro Lab - Mannitol Salt Agar
Mannitol Salt Agar - enriched, selecti…
Selective Mannitol Salt Agar % of salt…
Differential Mannitol Salt Agar colors
Gram + organism growth with no mannito…
Selective and differential
7.5% Salt and prevents all bacteria from growing except staph…
Sugar and phenol are red
Staphylococcus epidermidis
Mannitol Salt Agar - enriched, selecti…
Selective and differential
Selective Mannitol Salt Agar % of salt…
7.5% Salt and prevents all bacteria from growing except staph…
8 terms
Mannitol Salt Agar (ticklemeelmo1351)
Mannitol Salt Agar's selective agents
Mannitol Salt Agar's indicator
phenol red turns yellow with a possibl…
Mannitol Salt Agar is pink when pH =
7.5% NaCl
phenol red
acid
7.4-8.4
Mannitol Salt Agar's selective agents
7.5% NaCl
Mannitol Salt Agar's indicator
phenol red
12 terms
Microbiology Lab Mannitol Salt Agar
MSA contains what 3 things
below what pH is phenol red yellow?
pink at what pH?
red btw what pH's
carbohydrate manitol, sodium chloride (NaCl), and pH indicato…
below pH 6.8
pH 8.4 and above
7.4-8.4
MSA contains what 3 things
carbohydrate manitol, sodium chloride (NaCl), and pH indicato…
below what pH is phenol red yellow?
below pH 6.8
9 terms
LB6-4-3 Mannitol Salt Agar
3 Broth Cultures
Mannitol salt is both:
Selective Media
Differential Media
Escherichia Coli... Staphylococcus Epidermidis... Staphylococcus Au…
Selective media & Differential media
Favors growth of Staphylococcus species... Inhibits growth of Gr…
Isolate Staphylococcus aureus from other staph species
3 Broth Cultures
Escherichia Coli... Staphylococcus Epidermidis... Staphylococcus Au…
Mannitol salt is both:
Selective media & Differential media
11 terms
Exercise 4-3 Mannitol Salt Agar
Define selective media
Define differential media
What is the purpose of Mannitol Salt A…
Is Mannitol Salt Agar a selective or d…
designed to enhance the isolation procedure by inhibiting gro…
selective media contaning indicators to expose differences be…
favors growth of gram positive especially stap & inhibits gro…
both
Define selective media
designed to enhance the isolation procedure by inhibiting gro…
Define differential media
selective media contaning indicators to expose differences be…
8 terms
Lab Quiz 2 Mannitol Salt Agar
Mannitol Salt Agar contains the carboh…
Phenol red is yellow below pH ____, re…
Mannitol provides the substrate for __…
____ ____ makes the medium selective b…
MANNITOL, SODIUM CHLORIDE, PHENOL RED
6.8, 7.4, 8.4, 8.4
FERMENTATION, DIFFERENTIAL
SODIUM CHLORIDE
Mannitol Salt Agar contains the carboh…
MANNITOL, SODIUM CHLORIDE, PHENOL RED
Phenol red is yellow below pH ____, re…
6.8, 7.4, 8.4, 8.4
11 terms
micro lab quiz 2: mannitol salt agar
support the growth of
what is the selective agent?
what is the basis for differentiation?
what is the pH indicator?
g+ (staphlycoccus)
7.5% NaCL
the ability to ferment mannital
phenol red
support the growth of
g+ (staphlycoccus)
what is the selective agent?
7.5% NaCL
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